FastQCFastQC Report
Thu 26 May 2016
SRR1545759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545759_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences109420
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.2065435934929629No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.19649058673003109No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1590.1453116432096509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT207.005301E-445.0000048
GGATCCA207.005301E-445.0000048
ACGGGGC207.005301E-445.0000045
ACGGGCT207.005301E-445.0000045
ATATCAC302.1474752E-645.00000430
GTAGCAA207.005301E-445.0000041
ACAGGCT207.005301E-445.0000045
ATATAAT302.1474752E-645.00000437
CTCATGC351.1984957E-745.00000423
GCGATTG207.005301E-445.0000049
TATAGGC207.005301E-445.0000044
AGTTAAT302.1474752E-645.00000439
TGAGGCG207.005301E-445.0000045
ACGTAAG207.005301E-445.0000042
GGCGATT406.717528E-945.0000048
CGCAAGG207.005301E-445.0000043
AAGGCCT207.005301E-445.0000046
ACATAGT302.1474752E-645.00000435
TCGAAGG406.717528E-945.0000043
CGAAGGC302.1474752E-645.0000044