##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545759_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	109420
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.051498811917384	31.0	31.0	33.0	30.0	33.0
2	31.198336684335587	31.0	31.0	33.0	30.0	34.0
3	31.151160665326266	31.0	31.0	34.0	28.0	34.0
4	35.1387863279108	35.0	35.0	37.0	33.0	37.0
5	32.95409431548163	35.0	35.0	37.0	28.0	37.0
6	34.08994699323707	35.0	35.0	37.0	29.0	37.0
7	15.131411076585634	0.0	0.0	35.0	0.0	37.0
8	24.83692195211113	17.0	17.0	35.0	17.0	37.0
9	32.663909705721075	32.0	32.0	37.0	27.0	39.0
10	35.53982818497533	37.0	35.0	37.0	32.0	39.0
11	36.528184975324436	37.0	35.0	39.0	33.0	39.0
12	36.358764394077866	37.0	35.0	39.0	32.0	39.0
13	36.04298117346006	37.0	35.0	39.0	31.0	39.0
14	37.09065984280753	39.0	35.0	40.0	32.0	41.0
15	37.42172363370499	39.0	36.0	41.0	32.0	41.0
16	37.45704624383111	39.0	35.0	41.0	32.0	41.0
17	37.442176932919025	39.0	35.0	41.0	32.0	41.0
18	37.34496435752148	39.0	36.0	41.0	32.0	41.0
19	37.2879546700786	39.0	36.0	41.0	32.0	41.0
20	37.37856881740084	39.0	35.0	41.0	32.0	41.0
21	37.256031804057756	39.0	35.0	41.0	32.0	41.0
22	37.31104002924511	39.0	35.0	41.0	32.0	41.0
23	37.29528422591848	39.0	35.0	41.0	32.0	41.0
24	37.10775909340157	39.0	35.0	41.0	32.0	41.0
25	36.861158837506856	38.0	35.0	40.0	31.0	41.0
26	36.7641381831475	38.0	35.0	40.0	31.0	41.0
27	36.6629866569183	38.0	35.0	40.0	31.0	41.0
28	36.5457503198684	38.0	35.0	40.0	31.0	41.0
29	36.55632425516359	38.0	35.0	40.0	31.0	41.0
30	36.31776640467922	38.0	35.0	40.0	30.0	41.0
31	36.22852312191556	38.0	35.0	40.0	30.0	41.0
32	36.09772436483276	38.0	35.0	40.0	30.0	41.0
33	35.93864924145495	38.0	35.0	40.0	30.0	41.0
34	35.78112776457686	38.0	35.0	40.0	29.0	41.0
35	35.63064339243283	38.0	34.0	40.0	28.0	41.0
36	35.44400475233047	38.0	34.0	40.0	27.0	41.0
37	35.25580332663133	38.0	34.0	40.0	27.0	41.0
38	35.1505757631146	38.0	34.0	40.0	26.0	41.0
39	35.0572198866752	38.0	34.0	40.0	26.0	41.0
40	34.9022847742643	38.0	34.0	40.0	25.0	41.0
41	34.809522939133615	38.0	34.0	40.0	24.0	41.0
42	34.761195393895086	37.0	34.0	40.0	24.0	41.0
43	34.68679400475233	37.0	33.0	40.0	24.0	41.0
44	34.37637543410711	37.0	33.0	40.0	23.0	41.0
45	34.31891793090843	37.0	33.0	40.0	23.0	41.0
46	34.314101626759275	37.0	33.0	40.0	23.0	41.0
47	34.30977883385122	37.0	33.0	40.0	23.0	41.0
48	34.265061231950284	36.0	33.0	40.0	23.0	41.0
49	34.137872418205085	36.0	33.0	40.0	24.0	41.0
50	34.000932187899835	36.0	33.0	40.0	24.0	41.0
51	33.859906781210015	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	5.0
16	7.0
17	26.0
18	56.0
19	109.0
20	206.0
21	327.0
22	497.0
23	749.0
24	1073.0
25	1448.0
26	1826.0
27	1964.0
28	2157.0
29	2557.0
30	3121.0
31	3948.0
32	5398.0
33	8295.0
34	10670.0
35	11258.0
36	12921.0
37	19838.0
38	17470.0
39	3490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.477609212209835	24.819045878267225	28.375068543227925	14.32827636629501
2	33.0095046609395	23.52220800584902	30.149881191738253	13.318406141473222
3	30.67994882105648	24.068726009870225	31.988667519649063	13.262657649424236
4	28.206909157375254	27.06360811551819	29.383110948638276	15.346371778468287
5	21.942058124657287	35.90568451836958	28.157558033266312	13.994699323706818
6	21.06104916834217	41.69530250411259	27.33412538841162	9.909522939133614
7	37.793821970389324	57.43191372692378	4.013891427526961	0.7603728751599342
8	87.98665691829648	3.1639554012063607	7.029793456406506	1.81959422409066
9	83.22518735148967	5.237616523487479	8.623651983184061	2.9135441418387864
10	50.76859806251143	22.86693474684701	13.786327910802413	12.578139279839151
11	45.33631877170535	20.342716139645404	19.344726740997988	14.976238347651252
12	39.62346920124292	21.45403034180223	24.835496252970206	14.087004203984646
13	22.070005483458235	37.969292633887775	25.232133065253155	14.728568817400841
14	16.72546152440139	40.26137817583623	28.37689636263937	14.636263937123012
15	15.847194297203437	22.511423871321515	45.95229391336136	15.689087918113689
16	17.15042953756169	17.86510692743557	44.64174739535734	20.342716139645404
17	18.0789617985743	17.85048437214403	29.683787241820507	34.38676658746116
18	22.36428440870042	23.082617437397186	33.39151891793091	21.161579235971487
19	30.18186803143849	22.583622738073476	26.01078413452751	21.223725095960518
20	31.054651800402123	22.441966733686712	24.971668799122646	21.531712666788522
21	23.437214403216963	27.312191555474318	28.16395540120636	21.086638640102358
22	23.184975324437946	24.213123743374155	25.40394808992871	27.19795284225918
23	21.041857064522027	29.340157192469384	25.360994333759823	24.256991409248766
24	20.9193931639554	23.95265947724365	37.14677389873881	17.98117346006215
25	20.11058307439225	23.574300859075123	34.49826357155913	21.816852494973496
26	17.9921403765308	32.948272710656184	27.205264119904953	21.85432279290806
27	19.869310912081886	31.123195028331203	29.341071102175103	19.666422957411807
28	17.58362273807348	27.007859623469198	37.5150795101444	17.893438128312923
29	17.91628587095595	25.434107110217514	35.65801498811918	20.991592030707366
30	20.887406324255164	29.071467738987387	31.444891244745023	18.596234692012427
31	30.818863096326083	25.248583439956136	24.13087186985926	19.80168159385853
32	30.798757082800222	25.370133430817038	26.111314202156827	17.719795284225917
33	28.785414001096694	25.059404130871872	25.024675562054473	21.130506305976972
34	20.60318040577591	26.53719612502285	28.22335953207823	24.63626393712301
35	21.897276549076953	24.690184609760554	30.545604094315483	22.86693474684701
36	31.307804788886855	25.05666240175471	24.860171815024675	18.77536099433376
37	21.56369950648876	32.819411442149516	26.276731858892344	19.340157192469384
38	22.208919758727838	33.07073661122281	22.70243099981722	22.017912630232132
39	22.306708097239994	29.84554925973314	25.415828916103088	22.43191372692378
40	24.44799853774447	24.983549625297023	25.92853226101261	24.639919575945896
41	19.14915006397368	24.20946810455127	26.81045512703345	29.8309267044416
42	22.257356973131053	26.897276549076953	23.251690732955584	27.59367574483641
43	22.871504295375615	25.682690550173643	25.432279290806072	26.01352586364467
44	20.541034545786875	29.701151526229207	28.352220800584902	21.405593127399015
45	19.43063425333577	34.97715225735698	23.56242003290075	22.02979345640651
46	22.42186072016085	30.89654542131238	26.23195028331201	20.44964357521477
47	23.034180222993967	26.67611040029245	27.191555474319134	23.098153902394444
48	23.099067812100166	24.734966185340888	29.96618534088832	22.199780661670626
49	21.491500639736792	24.584171083896912	31.60756717236337	22.316761104002925
50	20.470663498446353	31.27216231036374	27.025223907877905	21.23195028331201
51	21.150612319502834	31.836958508499357	24.927801133248035	22.08462803874977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	97.0
2	140.0
3	524.0
4	908.0
5	726.5
6	545.0
7	534.0
8	523.0
9	529.0
10	535.0
11	566.0
12	597.0
13	542.0
14	487.0
15	451.5
16	416.0
17	384.5
18	353.0
19	374.0
20	395.0
21	427.0
22	459.0
23	486.0
24	513.0
25	589.5
26	818.0
27	970.0
28	1158.5
29	1347.0
30	1523.0
31	1699.0
32	1898.5
33	2098.0
34	2283.0
35	2468.0
36	2760.5
37	3053.0
38	4413.5
39	5774.0
40	7091.5
41	8409.0
42	9179.5
43	9950.0
44	9855.0
45	9760.0
46	9282.0
47	8804.0
48	8533.0
49	8262.0
50	7914.0
51	7566.0
52	7005.0
53	6444.0
54	6073.5
55	5703.0
56	5333.5
57	4964.0
58	4486.0
59	4008.0
60	3639.0
61	3270.0
62	2925.5
63	2581.0
64	2206.5
65	1832.0
66	1535.0
67	1238.0
68	1086.0
69	934.0
70	841.5
71	749.0
72	592.5
73	436.0
74	344.0
75	188.0
76	124.0
77	98.5
78	73.0
79	52.0
80	31.0
81	23.0
82	15.0
83	10.0
84	5.0
85	5.5
86	6.0
87	5.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	109420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.18387863279108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.59297248081461	31.769329190276004
2	13.339651039634317	13.121915554743191
3	7.499489009049186	11.065618716870773
4	4.842336064812234	9.526594772436484
5	3.2870654254231932	8.083531347102907
6	2.2390694390248433	6.607567172363371
7	1.4586468959622425	5.0219338329373056
8	0.8844788821376145	3.4801681593858524
9	0.5964658007692737	2.640285139828185
>10	1.254250515636323	8.134710290623287
>50	0.0	0.0
>100	0.005574446736161436	0.5483458234326449
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.2065435934929629	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.19649058673003109	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	159	0.1453116432096509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0027417291171632245	0.0
2	0.0	0.0	0.0	0.021933832937305796	0.0
3	0.0	0.0	0.0	0.03015902028879547	0.0
4	0.0	0.0	0.0	0.046609394991774813	0.0
5	0.0	0.0	0.0	0.0795101443977335	0.0
6	0.0	0.0	0.0	0.11880826174373972	0.0
7	0.0	0.0	0.0	0.15079510144397734	0.0
8	0.0	0.0	0.0	0.3555108755254981	0.0
9	0.0	0.0	0.0	0.45147139462621094	0.0
10	0.0	0.0	0.0	0.6114055931273991	0.0
11	0.0	0.0	0.0	0.7402668616340705	0.0
12	0.0	0.0	0.0	0.8755254980807896	0.0
13	0.0	0.0	0.0	0.9221348930725645	0.0
14	0.0	0.0	0.0	0.9340157192469384	0.0
15	0.0	0.0	0.0	0.9577773715956863	0.0
16	0.0	0.0	0.0	1.026320599524767	0.0
17	0.0	0.0	0.0	1.1058307439225004	0.0
18	0.0	0.0	0.0	1.2127581794918663	0.0
19	0.0	0.0	0.0	1.2520562968378723	0.0
20	0.0	0.0	0.0	1.3032352403582526	0.0
21	0.0	0.0	0.0	1.3589837324072382	0.0
22	0.0	0.0	0.0	1.4211295923962712	0.0
23	0.0	0.0	0.0	1.4878450009139097	0.0
24	0.0	0.0	0.0	1.540851763845732	0.0
25	0.0	0.0	0.0	1.5966002558947177	0.0
26	0.0	0.0	0.0	1.6532626576494243	0.0
27	0.0	0.0	0.0	1.699872052641199	0.0
28	0.0	0.0	0.0	1.7537927252787424	0.0
29	0.0	0.0	0.0	1.8113690367391702	0.0
30	0.0	0.0	0.0	1.8726009870224822	0.0
31	0.0	0.0	0.0	1.9365746664229575	0.0
32	0.0	0.0	0.0	1.9840979711204534	0.0
33	0.0	0.0	0.0	2.051727289343813	0.0
34	0.0	0.0	0.0	2.129409614330104	0.0
35	0.0	0.0	0.0	2.223542314019375	0.0
36	0.0	0.0	0.0	2.288429903125571	0.0
37	0.0	0.0	0.0	2.3661122281118625	0.0
38	0.0	0.0	0.0	2.433741546335222	0.0
39	0.0	0.0	0.0	2.5114238713215133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	20	7.005301E-4	45.000004	8
GGATCCA	20	7.005301E-4	45.000004	8
ACGGGGC	20	7.005301E-4	45.000004	5
ACGGGCT	20	7.005301E-4	45.000004	5
ATATCAC	30	2.1474752E-6	45.000004	30
GTAGCAA	20	7.005301E-4	45.000004	1
ACAGGCT	20	7.005301E-4	45.000004	5
ATATAAT	30	2.1474752E-6	45.000004	37
CTCATGC	35	1.1984957E-7	45.000004	23
GCGATTG	20	7.005301E-4	45.000004	9
TATAGGC	20	7.005301E-4	45.000004	4
AGTTAAT	30	2.1474752E-6	45.000004	39
TGAGGCG	20	7.005301E-4	45.000004	5
ACGTAAG	20	7.005301E-4	45.000004	2
GGCGATT	40	6.717528E-9	45.000004	8
CGCAAGG	20	7.005301E-4	45.000004	3
AAGGCCT	20	7.005301E-4	45.000004	6
ACATAGT	30	2.1474752E-6	45.000004	35
TCGAAGG	40	6.717528E-9	45.000004	3
CGAAGGC	30	2.1474752E-6	45.000004	4
>>END_MODULE