##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545757_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	428342
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01426196824033	31.0	31.0	33.0	30.0	33.0
2	31.17798394740651	31.0	31.0	33.0	30.0	34.0
3	31.12074463862988	31.0	31.0	34.0	28.0	34.0
4	35.08698890139188	35.0	35.0	37.0	32.0	37.0
5	32.77458199289353	35.0	35.0	37.0	28.0	37.0
6	33.96639134149815	35.0	35.0	37.0	28.0	37.0
7	15.445113951001769	0.0	0.0	35.0	0.0	37.0
8	24.997912882696536	17.0	17.0	35.0	17.0	37.0
9	32.77384659921278	32.0	32.0	37.0	27.0	39.0
10	35.55487904524889	37.0	35.0	37.0	32.0	39.0
11	36.49329741188116	37.0	35.0	39.0	32.0	39.0
12	36.22539933044156	37.0	35.0	39.0	32.0	39.0
13	35.76911439924173	37.0	35.0	39.0	31.0	39.0
14	36.78009861279072	39.0	35.0	40.0	31.0	41.0
15	37.194337702116535	39.0	35.0	41.0	32.0	41.0
16	37.29496290347433	39.0	35.0	41.0	32.0	41.0
17	37.27168477524968	39.0	35.0	41.0	32.0	41.0
18	37.19521550536721	39.0	35.0	40.0	32.0	41.0
19	37.099278146901305	39.0	35.0	40.0	32.0	41.0
20	37.206206722665534	39.0	35.0	40.0	32.0	41.0
21	37.02620336086585	39.0	35.0	40.0	32.0	41.0
22	37.094578631093846	39.0	35.0	40.0	32.0	41.0
23	37.09022930275341	39.0	35.0	40.0	32.0	41.0
24	36.91244846407777	38.0	35.0	40.0	32.0	41.0
25	36.66157416270177	38.0	35.0	40.0	31.0	41.0
26	36.55210789509317	38.0	35.0	40.0	31.0	41.0
27	36.439277493218036	38.0	35.0	40.0	31.0	41.0
28	36.390304943246285	38.0	35.0	40.0	31.0	41.0
29	36.39704488469494	38.0	35.0	40.0	31.0	41.0
30	36.15921389917403	38.0	35.0	40.0	30.0	41.0
31	36.00623800607926	38.0	34.0	40.0	30.0	41.0
32	35.85024583160185	38.0	34.0	40.0	30.0	41.0
33	35.64995494254591	38.0	34.0	40.0	29.0	41.0
34	35.514145239084655	38.0	34.0	40.0	28.0	41.0
35	35.31919587619239	38.0	34.0	40.0	27.0	41.0
36	35.1315794388596	38.0	34.0	40.0	26.0	41.0
37	34.9723001713584	38.0	34.0	40.0	25.0	41.0
38	34.89045435656555	38.0	34.0	40.0	25.0	41.0
39	34.82510704063576	38.0	34.0	40.0	25.0	41.0
40	34.66044655905795	37.0	33.0	40.0	24.0	41.0
41	34.547856619243504	37.0	33.0	40.0	24.0	41.0
42	34.50619131441699	37.0	33.0	40.0	23.0	41.0
43	34.41667405951319	37.0	33.0	40.0	23.0	41.0
44	34.16344649835879	37.0	33.0	40.0	23.0	41.0
45	34.05306040500348	36.0	33.0	40.0	23.0	41.0
46	34.057755251644714	36.0	33.0	40.0	23.0	41.0
47	34.02540493344104	36.0	33.0	40.0	23.0	41.0
48	34.011866685965884	36.0	33.0	40.0	23.0	41.0
49	33.929210770832654	36.0	33.0	40.0	23.0	41.0
50	33.79144001755606	36.0	33.0	40.0	23.0	41.0
51	33.57761321560809	36.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	5.0
15	12.0
16	51.0
17	114.0
18	287.0
19	495.0
20	861.0
21	1411.0
22	2328.0
23	3263.0
24	4674.0
25	6246.0
26	7648.0
27	8321.0
28	9141.0
29	10509.0
30	12772.0
31	16404.0
32	22456.0
33	33939.0
34	42922.0
35	43669.0
36	47848.0
37	73192.0
38	67090.0
39	12679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.160283138240004	24.44728744788043	29.149371296767534	14.243058117112028
2	32.45093873586993	24.031731653678605	30.812061390197552	12.70526822025391
3	29.48461743186519	22.87237767951777	34.311601477324196	13.331403411292845
4	27.264895807555646	26.998986790928743	30.616423325286803	15.119694076228807
5	21.135214384767313	36.952481895307955	28.70720125507188	13.205102464852853
6	20.2172563045417	40.81084740697853	28.218806467729056	10.753089820750708
7	38.29159876920779	56.63068295894402	4.287228429619323	0.7904898422288732
8	88.42677113147906	2.8145734016276713	7.124914204070579	1.6337412628226977
9	84.14911449262506	4.442478206666635	8.758188550270578	2.6502187504377344
10	54.79826867316303	21.457620312740755	13.37856199018541	10.3655490239108
11	48.983755970696315	18.993934753071144	19.0273192915941	12.994989984638444
12	43.77997021071947	20.16612893435619	23.178441525696755	12.875459329227578
13	21.493572892688555	41.56234971121207	23.883952542594468	13.06012485350491
14	14.74172507015422	43.49911986216621	27.67531551890779	14.083839548771776
15	13.173819051132037	22.514486088219225	49.80739689313679	14.504297967511942
16	15.261636729529208	16.117728357247245	48.8455486503775	19.77508626284604
17	15.614158779666715	16.99086244169379	30.258531733988264	37.13644704465124
18	21.57271526023598	21.8141111541712	35.68223522325618	20.930938362336637
19	29.68329045482348	23.24124181144973	26.24771794500656	20.82774978872023
20	32.16635305433509	21.879012564726317	25.201824710161507	20.75280967077709
21	22.733469984264907	28.190791470367138	27.731578971942984	21.344159573424974
22	22.363205102464853	23.502481661849643	24.974669773218597	29.159643462466907
23	18.8494240583459	30.195031073301244	25.548743760826632	25.406801107526228
24	20.01438103197912	22.838759682683463	39.924639657096435	17.222219628240985
25	17.94197160213101	23.206456523058677	37.33185165125064	21.51972022355968
26	16.422391453558138	33.859859644863214	28.078498022608102	21.639250878970543
27	17.882906649359622	33.32057094564624	29.590140588595094	19.206381816399045
28	15.7612375158168	27.04170966190567	39.47966811566459	17.71738470661294
29	15.57330357518058	24.585027851576545	38.61797348847416	21.223695084768714
30	19.01517945940393	29.831536482530314	31.906047037180567	19.24723702088518
31	30.973614541651294	24.673742009889292	24.7419118368033	19.610731611656107
32	31.75826792609644	25.887258312283173	25.236609998552556	17.117863763067827
33	28.87108898963912	25.836831317031717	25.773097198033344	19.518982495295813
34	20.101927898735124	26.297911481946667	28.3462747057258	25.253885913592413
35	20.427835701378804	24.43398032413352	32.21584621634115	22.922337758146526
36	30.71727731578972	23.792670342856876	26.02009609144095	19.469956249912453
37	19.998739325118713	32.52494502056768	27.80301721521588	19.67329843909773
38	21.333653949414252	33.597452502906556	23.15556261118452	21.91333093649467
39	20.74557246312526	31.112989153526854	25.83916590014521	22.302272483202675
40	24.218965219380774	24.98797689696551	25.552479093808216	25.240578789845497
41	17.463382063864856	23.403261879526173	27.18738764818766	31.94596840842131
42	21.629912546516568	25.905701518879777	23.618276984278918	28.84610895032474
43	22.263985320141384	25.539171970061307	25.231240457391525	26.96560225240579
44	20.562541147027375	29.299018074342463	28.771635749004297	21.36680502962586
45	18.219786992636724	36.677934921161125	23.005448916986893	22.096829169215255
46	22.00554696947766	31.590411400236263	27.039141620480834	19.36490000980525
47	22.422036596924887	27.08886824079824	26.377520766116795	24.111574396160076
48	22.0620438808242	24.32448837611068	31.021473495477913	22.59199424758721
49	20.659426346237353	23.675707728870858	32.69280154642785	22.972064378463937
50	19.480928790545875	31.869860998921425	27.15913919251439	21.490071018018313
51	18.862264265470117	33.54025521662597	24.916305195381263	22.681175322522655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	465.5
2	607.0
3	2328.5
4	4050.0
5	3081.0
6	2112.0
7	2148.5
8	2185.0
9	2272.0
10	2359.0
11	2332.5
12	2306.0
13	2216.0
14	2126.0
15	1994.0
16	1862.0
17	1726.0
18	1590.0
19	1597.5
20	1605.0
21	1599.0
22	1593.0
23	1725.5
24	1858.0
25	2160.0
26	2934.0
27	3406.0
28	4030.5
29	4655.0
30	5553.0
31	6451.0
32	7392.0
33	8333.0
34	8821.0
35	9309.0
36	10637.0
37	11965.0
38	17099.0
39	22233.0
40	29804.0
41	37375.0
42	39960.0
43	42545.0
44	41084.0
45	39623.0
46	39375.5
47	39128.0
48	36981.5
49	34835.0
50	32269.5
51	29704.0
52	26684.5
53	23665.0
54	21361.0
55	19057.0
56	17915.5
57	16774.0
58	15737.5
59	14701.0
60	13275.5
61	11850.0
62	10237.5
63	8625.0
64	7179.5
65	5734.0
66	4786.5
67	3839.0
68	3344.0
69	2849.0
70	2382.0
71	1915.0
72	1558.0
73	1201.0
74	989.0
75	586.5
76	396.0
77	295.0
78	194.0
79	148.0
80	102.0
81	69.5
82	37.0
83	27.5
84	18.0
85	10.0
86	2.0
87	1.0
88	0.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.463004618441836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97036042143361	20.9100005686918
2	7.051946646053898	4.155430732033828
3	3.7403644455919665	3.306071248053952
4	2.4815787286439748	2.924590621722577
5	1.813131040235524	2.6710144106149745
6	1.5172019643059234	2.6820797088872665
7	1.2643735522618407	2.6076570666808423
8	1.0736897622501547	2.5307301139160017
9	0.953482398509447	2.528321067979815
>10	8.94175386816939	50.79663756032971
>50	0.16998021439101169	3.2503914373176657
>100	0.019765141208257172	0.8492853074717078
>500	7.906056483302869E-4	0.2296750721796774
>1k	0.0015812112966605738	0.5581150841201897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1254	0.29275672243207534	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1142	0.266609391560949	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	986	0.23018989499045153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011672915567467117	0.0
2	0.0	0.0	0.0	0.05369541161034874	0.0
3	0.0	0.0	0.0	0.06606870211186389	0.0
4	0.0	0.0	0.0	0.0959513659645797	0.0
5	0.0	0.0	0.0	0.15548323535866201	0.0
6	0.0	0.0	0.0	0.2259876453861634	0.0
7	0.0	0.0	0.0	0.28085034855325886	0.0
8	0.0	0.0	0.0	0.6083923593763861	0.0
9	0.0	0.0	0.0	0.7480004295632929	0.0
10	0.0	0.0	0.0	0.9807583659785872	0.0
11	0.0	0.0	0.0	1.1691592232375065	0.0
12	0.0	0.0	0.0	1.3559258723169803	0.0
13	0.0	0.0	0.0	1.4289983237693245	0.0
14	0.0	0.0	0.0	1.4521106965929094	0.0
15	0.0	0.0	0.0	1.4950670258811884	0.0
16	0.0	0.0	0.0	1.5872830588641786	0.0
17	0.0	0.0	0.0	1.7166189633517144	0.0
18	0.0	0.0	0.0	1.9220622773391356	0.0
19	0.0	0.0	0.0	1.9937339789233837	0.0
20	0.0	0.0	0.0	2.0712421382913653	0.0
21	0.0	0.0	0.0	2.176765295021268	0.0
22	0.0	0.0	0.0	2.2813546185057736	0.0
23	0.0	0.0	0.0	2.4060213567663222	0.0
24	0.0	0.0	0.0	2.4893659739180376	0.0
25	0.0	0.0	0.0	2.5596369256341895	0.0
26	0.0	0.0	0.0	2.6317755438411363	0.0
27	0.0	0.0	0.0	2.7022799538686377	0.0
28	0.0	0.0	0.0	2.780255029859318	0.0
29	0.0	0.0	0.0	2.8575297309159504	0.0
30	0.0	0.0	0.0	2.9728581367225253	0.0
31	0.0	0.0	0.0	3.0797820433205243	0.0
32	0.0	0.0	0.0	3.167562368387877	0.0
33	0.0	0.0	0.0	3.2546423185211815	0.0
34	0.0	0.0	0.0	3.3522278926652067	0.0
35	0.0	0.0	0.0	3.4897348380499693	0.0
36	0.0	0.0	0.0	3.5884877037507414	0.0
37	0.0	0.0	0.0	3.6921431939898492	0.0
38	0.0	0.0	0.0	3.7871607267090317	0.0
39	0.0	0.0	0.0	3.891049675259489	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	85	0.0	45.0	25
TTTAGCG	35	1.209537E-7	45.0	1
TTAATCG	40	6.7975634E-9	45.0	20
GGTAAGT	25	3.8866936E-5	45.0	8
GTACAAC	20	7.0283253E-4	45.0	21
ATAGGCC	40	6.7975634E-9	45.0	5
ACGTTAT	20	7.0283253E-4	45.0	15
CGCATCG	20	7.0283253E-4	45.0	21
CGTTGAT	40	6.7975634E-9	45.0	25
GATCCAC	20	7.0283253E-4	45.0	23
TTACTAC	20	7.0283253E-4	45.0	27
CAATTGC	25	3.8866936E-5	45.0	25
CGATTCG	25	3.8866936E-5	45.0	10
TAACGGC	20	7.0283253E-4	45.0	18
ATCGTTG	50	2.1827873E-11	45.0	23
CGTTATC	20	7.0283253E-4	45.0	16
ACTAGGT	20	7.0283253E-4	45.0	4
CCGTAAC	20	7.0283253E-4	45.0	15
GTGTCGA	20	7.0283253E-4	45.0	1
TGTTACG	25	3.8866936E-5	45.0	1
>>END_MODULE