FastQCFastQC Report
Thu 26 May 2016
SRR1545756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545756_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1413688
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.3061495888767536No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21390.1513063702882107No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.1370175031548687No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.1253459037637725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA207.857096E-444.00000439
ACGTAAC207.857096E-444.00000439
CCAACGG207.857096E-444.0000042
TATACGC207.857096E-444.00000412
TCGAATA351.4464058E-744.01
CGTTTTA23400.043.247861
CGTTAAG1100.042.02
CGGTCTA3300.042.031
CGTTATT13850.041.776171
CTACGGG1800.041.5555573
AGGCGAT10150.041.182277
AGTACGG1500.041.0666662
CACGACG3450.040.81159626
CGACGGT3400.040.76470628
ATAACGG1250.040.482
TCACGAC3450.040.17391625
GTACGGG4050.039.654323
CAAGGAC9500.039.136845
TCACGGG1800.039.111113
ACGGTCT3600.039.1111130
Produced by FastQC (version 0.11.5)