##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545756_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1413688 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.515623673681887 31.0 31.0 33.0 30.0 34.0 2 31.70273497405368 31.0 31.0 34.0 30.0 34.0 3 31.782891274453767 31.0 31.0 34.0 30.0 34.0 4 35.6976242282597 37.0 35.0 37.0 35.0 37.0 5 27.12488328400609 35.0 25.0 37.0 0.0 37.0 6 31.35657867931255 35.0 26.0 37.0 17.0 37.0 7 14.968593494462711 0.0 0.0 32.0 0.0 37.0 8 25.078165054807002 17.0 17.0 35.0 17.0 37.0 9 33.180532762533176 32.0 32.0 37.0 28.0 39.0 10 35.97752545116037 37.0 35.0 39.0 32.0 39.0 11 36.9819656105166 38.0 37.0 39.0 34.0 39.0 12 37.03879781111532 39.0 35.0 39.0 33.0 39.0 13 36.6085953902134 39.0 35.0 39.0 33.0 39.0 14 37.92753917413177 40.0 36.0 41.0 33.0 41.0 15 38.18675478606312 40.0 37.0 41.0 33.0 41.0 16 38.249705734221415 40.0 37.0 41.0 34.0 41.0 17 38.266104685050735 40.0 37.0 41.0 34.0 41.0 18 38.24610522265167 40.0 36.0 41.0 34.0 41.0 19 38.19935586918754 40.0 37.0 41.0 34.0 41.0 20 38.24175489924227 40.0 37.0 41.0 34.0 41.0 21 38.12159401508678 40.0 36.0 41.0 34.0 41.0 22 38.06162250793669 40.0 36.0 41.0 34.0 41.0 23 37.969192636564784 40.0 36.0 41.0 34.0 41.0 24 37.84816239509708 40.0 35.0 41.0 34.0 41.0 25 37.66681049849755 40.0 35.0 41.0 33.0 41.0 26 37.44026687642535 39.0 35.0 41.0 33.0 41.0 27 37.250867235203245 39.0 35.0 40.0 33.0 41.0 28 37.26620371680314 39.0 35.0 40.0 33.0 41.0 29 37.19435830253917 39.0 35.0 40.0 33.0 41.0 30 37.014855470231055 39.0 35.0 40.0 32.0 41.0 31 36.87662341336985 39.0 35.0 40.0 32.0 41.0 32 36.70917416006927 39.0 35.0 40.0 31.0 41.0 33 36.558752001856135 39.0 35.0 40.0 31.0 41.0 34 36.349309748685705 38.0 35.0 40.0 31.0 41.0 35 36.20980301169706 38.0 35.0 40.0 30.0 41.0 36 36.02703425366842 38.0 35.0 40.0 30.0 41.0 37 35.906593251127546 38.0 35.0 40.0 30.0 41.0 38 35.72786923281516 38.0 35.0 40.0 30.0 41.0 39 35.679386116314205 38.0 35.0 40.0 29.0 41.0 40 35.43942652126919 38.0 34.0 40.0 29.0 41.0 41 35.241877981563114 38.0 34.0 40.0 28.0 41.0 42 35.245908573886176 38.0 34.0 40.0 28.0 41.0 43 35.1405012987307 38.0 34.0 40.0 28.0 41.0 44 34.93002699322623 37.0 34.0 40.0 27.0 40.0 45 34.70267484763257 37.0 34.0 40.0 27.0 40.0 46 34.64766907549615 37.0 34.0 40.0 27.0 40.0 47 34.476520278873416 37.0 33.0 39.0 26.0 40.0 48 34.396495549230096 36.0 33.0 39.0 26.0 40.0 49 34.24452142198279 36.0 33.0 39.0 26.0 40.0 50 34.34886905738749 36.0 33.0 39.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 8.0 14 14.0 15 28.0 16 63.0 17 165.0 18 388.0 19 810.0 20 1445.0 21 2569.0 22 4278.0 23 6413.0 24 9680.0 25 14055.0 26 18007.0 27 20281.0 28 22472.0 29 26275.0 30 33160.0 31 44397.0 32 62230.0 33 109921.0 34 116970.0 35 147410.0 36 203134.0 37 367468.0 38 160254.0 39 41790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.80144699537663 23.965542609118845 28.250717272835306 13.982293122669217 2 33.05885032623889 24.504629027055476 30.000891285771687 12.435629360933955 3 30.96538981727227 24.104611484287904 32.046816553581834 12.883182144857988 4 27.861168801036722 26.932250963437475 29.65894879209557 15.547631443430236 5 17.355597557594038 48.37821358036568 22.75247437907091 11.513714482969368 6 20.30631935759517 41.80809344070261 27.444457334291585 10.44112986741063 7 39.788270113348915 55.75933303529491 3.3830661362337375 1.0693307151224316 8 88.37063057760976 2.9647277192704475 6.745547815359541 1.9190938877602413 9 83.72349485883731 4.626409787732513 8.38756500727176 3.262530346158417 10 47.280658815806596 24.383032182490055 15.010101238745749 13.326207762957598 11 40.623815155819386 22.441514676505708 20.986526022715054 15.94814414495985 12 37.07480009733406 21.346647916654877 26.107033518003973 15.471518468007083 13 23.42270713198386 33.08275942074913 26.697050551465384 16.79748289580162 14 17.844389992699945 36.75726185693024 28.69791637192931 16.700431778440507 15 16.784042872260358 23.531217637838054 42.17925030133948 17.505489188562116 16 18.825653185144105 18.42407942912439 41.981823429214934 20.76844395651657 17 19.2333810572064 18.72902648957903 29.604198380406427 32.43339407280815 18 21.825890861349887 23.74979486279858 32.73586533945255 21.68844893639898 19 28.4721239764361 24.895804449072216 25.574101216109916 21.057970358381763 20 30.33611376767717 23.548618931475687 24.685927870930502 21.429339429916645 21 24.35127128475307 26.28790794008296 28.24237031084652 21.118450464317444 22 23.895371538840255 23.13466620640481 26.344073091092234 26.625889163662702 23 21.731669222629037 28.485493263011357 25.468773873726025 24.314063640633577 24 21.798161970675284 24.459994001505283 34.38064127303903 19.361202754780404 25 21.371971750485255 23.969079457419177 32.44336798501508 22.21558080708049 26 19.12925624324462 30.132037620748005 27.555726581820032 23.182979554187348 27 20.122827667773933 29.02302346769584 29.029884953398486 21.824263911131737 28 18.622779566637053 26.92701642795299 34.687498231575844 19.762705773834114 29 18.857909241643135 25.129236436894136 34.38191453842715 21.630939783035576 30 21.156011793266973 26.920437890114368 31.408698383235905 20.514851933382754 31 28.017709706809423 25.48306274085937 25.455263113218756 21.04396443911245 32 28.0376575312233 26.1158049017888 26.257066622904063 19.589470944083843 33 27.40463242242984 25.546089377571292 25.71861683766149 21.330661362337374 34 21.274213263464073 26.278995082366123 28.241733678152464 24.20505797601734 35 21.761025063521796 24.98380123478448 29.986956103468376 23.268217598225352 36 28.619822761457975 25.165524500455543 25.703974285698116 20.510678452388362 37 22.29388662844984 30.227461787890963 26.585852040902942 20.89279954275625 38 22.508997742076044 30.459054614596713 24.65437918409154 22.3775684592357 39 22.188842233929975 28.04501417568799 25.711543141060826 24.05460044932121 40 24.151156407920276 24.74605429203615 27.42281182269355 23.679977477350022 41 20.116532077799345 24.324815659466587 27.50840355156159 28.050248711172483 42 22.6085953902134 27.104919897459695 24.23413086904607 26.052353843280834 43 22.273655856171942 26.19651578000238 25.557124344268324 25.97270401955736 44 21.67578701948379 27.509676816949707 28.550076112975425 22.26446005059108 45 20.493843054478784 31.291133545732862 25.150599000628144 23.06442439916021 46 22.400699447119873 29.648055299330544 27.42429729897969 20.52694795456989 47 22.782537589623736 27.119420975491053 27.258489850660116 22.83955158422509 48 22.651179043749398 25.664786006530438 28.364603788106002 23.31943116161416 49 21.554756070646423 24.91617669528213 30.935892502447498 22.593174731623954 50 21.1925120677264 28.89704093123801 27.682840909733976 22.227606091301617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 718.0 1 1443.5 2 2169.0 3 7826.5 4 13484.0 5 9757.0 6 6030.0 7 6147.5 8 6265.0 9 6306.0 10 6347.0 11 6278.5 12 6210.0 13 5904.0 14 5598.0 15 5289.5 16 4981.0 17 5026.0 18 5071.0 19 4866.5 20 4662.0 21 5201.5 22 5741.0 23 6583.5 24 7426.0 25 8743.5 26 10061.0 27 12026.0 28 13991.0 29 17131.5 30 20272.0 31 23542.5 32 26813.0 33 29930.0 34 33047.0 35 36440.0 36 39833.0 37 45910.5 38 51988.0 39 64436.0 40 76884.0 41 93165.5 42 109447.0 43 113094.5 44 116742.0 45 117780.5 46 118819.0 47 117980.5 48 117142.0 49 113460.5 50 109779.0 51 101049.0 52 92319.0 53 84683.5 54 77048.0 55 73621.5 56 70195.0 57 67100.5 58 64006.0 59 60078.5 60 56151.0 61 49065.5 62 41980.0 63 35797.5 64 29615.0 65 25294.5 66 20974.0 67 18571.5 68 16169.0 69 13676.5 70 11184.0 71 9080.0 72 6976.0 73 5435.5 74 3895.0 75 2936.5 76 1978.0 77 1429.5 78 881.0 79 637.5 80 394.0 81 299.5 82 205.0 83 159.0 84 113.0 85 85.0 86 57.0 87 34.0 88 11.0 89 9.5 90 8.0 91 6.0 92 4.0 93 3.5 94 3.0 95 2.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1413688.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.954302671887568 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61600084093746 16.209410567811457 2 6.9401471885085515 3.186124783049497 3 3.33531455681967 2.296794595295738 4 2.1394508761404123 1.9643841185024828 5 1.645403195472317 1.8884541483081279 6 1.310716579823698 1.8051951054200668 7 1.1394948475143512 1.8309416736030555 8 0.9683823205890689 1.778283271112508 9 0.837905901360409 1.731019110334884 >10 10.095651995459114 50.047934600245945 >50 0.8865491074289423 13.011064409283978 >100 0.08043619624534647 2.985880042042252 >500 0.0031475033312879873 0.447621108058931 >1k 0.0013988903694613277 0.8168924669310764 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.3061495888767536 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 2139 0.1513063702882107 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.1370175031548687 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1772 0.1253459037637725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011388651527069622 0.0 2 0.0 0.0 0.0 0.03395374368318894 0.0 3 0.0 0.0 0.0 0.0454131321762652 0.0 4 0.0 0.0 0.0 0.06444137603205233 0.0 5 0.0 0.0 0.0 0.10568102721392557 0.0 6 0.0 0.0 0.0 0.19424370865424337 0.0 7 0.0 0.0 0.0 0.2513991771876114 0.0 8 0.0 0.0 0.0 0.5406426311887772 0.0 9 0.0 0.0 0.0 0.6666251676466094 0.0 10 0.0 0.0 0.0 0.8857682883351914 0.0 11 0.0 0.0 0.0 1.0623984924537804 0.0 12 0.0 0.0 0.0 1.2334404762578448 0.0 13 0.0 0.0 0.0 1.301065015760196 0.0 14 0.0 0.0 0.0 1.3268132713866143 0.0 15 0.0 0.0 0.0 1.363384282812049 0.0 16 0.0 0.0 0.0 1.458879186921018 0.0 17 0.0 0.0 0.0 1.5659042164890697 0.0 18 0.0 0.0 0.0 1.7360266197350476 0.0 19 0.0 0.0 0.0 1.8009631545291465 0.0 20 0.0 0.0 0.0 1.873892966481996 0.0 21 0.0 0.0 0.0 1.9724295601292505 0.0 22 0.0 0.0 0.0 2.0698343623203987 0.0 23 0.0 0.0 0.0 2.186479619265354 0.0 24 0.0 0.0 0.0 2.2749008267736586 0.0 25 0.0 0.0 0.0 2.3435864207661097 0.0 26 0.0 0.0 0.0 2.4124842256565806 0.0 27 0.0 0.0 0.0 2.4864750920995298 0.0 28 0.0 0.0 0.0 2.560890380338519 0.0 29 0.0 0.0 0.0 2.650160431438903 0.0 30 0.0 0.0 0.0 2.7574684088709813 0.0 31 0.0 0.0 0.0 2.8589052181245083 0.0 32 0.0 0.0 0.0 2.9573710748057565 0.0 33 0.0 0.0 0.0 3.0500365002744596 0.0 34 0.0 0.0 0.0 3.1479364612276544 0.0 35 0.0 0.0 0.0 3.2783046895778982 0.0 36 0.0 0.0 0.0 3.3812269751175648 0.0 37 0.0 0.0 0.0 3.491364431189909 0.0 38 0.0 0.0 0.0 3.599521252214067 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCGA 20 7.857096E-4 44.000004 39 ACGTAAC 20 7.857096E-4 44.000004 39 CCAACGG 20 7.857096E-4 44.000004 2 TATACGC 20 7.857096E-4 44.000004 12 TCGAATA 35 1.4464058E-7 44.0 1 CGTTTTA 2340 0.0 43.24786 1 CGTTAAG 110 0.0 42.0 2 CGGTCTA 330 0.0 42.0 31 CGTTATT 1385 0.0 41.77617 1 CTACGGG 180 0.0 41.555557 3 AGGCGAT 1015 0.0 41.18227 7 AGTACGG 150 0.0 41.066666 2 CACGACG 345 0.0 40.811596 26 CGACGGT 340 0.0 40.764706 28 ATAACGG 125 0.0 40.48 2 TCACGAC 345 0.0 40.173916 25 GTACGGG 405 0.0 39.65432 3 CAAGGAC 950 0.0 39.13684 5 TCACGGG 180 0.0 39.11111 3 ACGGTCT 360 0.0 39.11111 30 >>END_MODULE