FastQCFastQC Report
Thu 26 May 2016
SRR1545755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545755_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences543715
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.20157619341015054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1863108429967906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8030.14768766725214497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG502.1827873E-1145.0000043
ATAACGG253.8880837E-545.0000042
CGGGTAC207.0300046E-445.06
ATCTCGT453.8380676E-1045.019
CGAACTA207.0300046E-445.038
TACGGGA551.8189894E-1245.04
GCGCATA207.0300046E-445.01
TACGCAT207.0300046E-445.045
TAGTGCG207.0300046E-445.01
CGTTAAG302.1633841E-644.9999962
AGGCGAT3600.044.3757
CGACGGT1200.043.12499628
GACGGTC1200.043.12499629
CGGTCTA1150.043.04347631
CGTTTTA6500.042.9230731
CGTTTTT8550.042.6315771
TCACGAC1300.041.5384625
ACGGTCT1250.041.430
GCGCGTC501.0786607E-940.50000438
TGCGCGT501.0786607E-940.50000437