##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545755_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	543715
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.004781916996958	31.0	31.0	33.0	30.0	33.0
2	31.159846610816327	31.0	31.0	33.0	30.0	34.0
3	31.121718179560983	31.0	31.0	34.0	28.0	34.0
4	35.135274914247354	35.0	35.0	37.0	33.0	37.0
5	32.84187487930257	35.0	35.0	37.0	28.0	37.0
6	34.01772987686564	35.0	35.0	37.0	28.0	37.0
7	15.273121028479995	0.0	0.0	35.0	0.0	37.0
8	24.88976945642478	17.0	17.0	35.0	17.0	37.0
9	32.67733831143154	32.0	32.0	37.0	27.0	39.0
10	35.43410426418252	37.0	34.0	37.0	32.0	39.0
11	36.420696504602596	37.0	35.0	39.0	32.0	39.0
12	36.35198771415172	37.0	35.0	39.0	32.0	39.0
13	36.10542655619212	37.0	35.0	39.0	32.0	39.0
14	37.14092493309914	39.0	36.0	40.0	32.0	41.0
15	37.45722299366396	39.0	36.0	41.0	32.0	41.0
16	37.478905308847466	39.0	36.0	41.0	32.0	41.0
17	37.4238286602356	39.0	35.0	41.0	32.0	41.0
18	37.352949615147644	39.0	36.0	41.0	32.0	41.0
19	37.315748140110166	39.0	36.0	40.0	32.0	41.0
20	37.458102130711865	39.0	35.0	41.0	32.0	41.0
21	37.32645227738797	39.0	35.0	41.0	32.0	41.0
22	37.39655518056335	39.0	35.0	41.0	32.0	41.0
23	37.37662010428257	39.0	35.0	41.0	32.0	41.0
24	37.22924142243639	39.0	35.0	41.0	32.0	41.0
25	36.96179248319432	39.0	35.0	40.0	31.0	41.0
26	36.867290768141395	39.0	35.0	40.0	31.0	41.0
27	36.788940897345114	39.0	35.0	40.0	31.0	41.0
28	36.686861683050864	38.0	35.0	40.0	31.0	41.0
29	36.664883256853315	38.0	35.0	40.0	31.0	41.0
30	36.41389514727385	38.0	35.0	40.0	30.0	41.0
31	36.32251823105855	38.0	35.0	40.0	30.0	41.0
32	36.27016543593611	38.0	35.0	40.0	30.0	41.0
33	36.13806313969635	38.0	35.0	40.0	30.0	41.0
34	35.99581398342882	38.0	35.0	40.0	29.0	41.0
35	35.8416468186458	38.0	35.0	40.0	29.0	41.0
36	35.64951675050348	38.0	34.0	40.0	28.0	41.0
37	35.51848854638919	38.0	34.0	40.0	27.0	41.0
38	35.404552017141334	38.0	34.0	40.0	27.0	41.0
39	35.31426574584111	38.0	34.0	40.0	27.0	41.0
40	35.157297481217185	38.0	34.0	40.0	26.0	41.0
41	35.017831032802114	38.0	34.0	40.0	26.0	41.0
42	34.969548384723616	38.0	34.0	40.0	25.0	41.0
43	34.89552247041189	38.0	34.0	40.0	25.0	41.0
44	34.57819261929504	37.0	33.0	40.0	24.0	41.0
45	34.506793080933946	37.0	33.0	40.0	24.0	41.0
46	34.499344325611766	37.0	33.0	40.0	24.0	41.0
47	34.44639930846123	37.0	33.0	40.0	24.0	41.0
48	34.403693111280724	37.0	33.0	40.0	24.0	41.0
49	34.301786781677905	37.0	33.0	40.0	24.0	41.0
50	34.190266959712346	36.0	33.0	40.0	24.0	41.0
51	34.00636730640133	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	1.0
14	12.0
15	20.0
16	51.0
17	134.0
18	270.0
19	580.0
20	938.0
21	1545.0
22	2417.0
23	3525.0
24	5178.0
25	6697.0
26	8081.0
27	9293.0
28	10311.0
29	12504.0
30	15399.0
31	19905.0
32	26706.0
33	39307.0
34	49997.0
35	55219.0
36	67298.0
37	101556.0
38	87957.0
39	18808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.234874888498574	24.676346983254092	28.451670452350957	13.63710767589638
2	32.87420799499738	24.18822360979557	30.545414417479744	12.392153977727302
3	31.36919158014769	23.83932758890227	31.798276670682252	12.993204160267787
4	28.317592856551688	27.435513090497782	28.549699750788555	15.697194302161979
5	21.168810865986774	36.99714004579605	27.66780390461915	14.166245183598026
6	20.221439540935968	42.48696467818618	27.09838794221237	10.193207838665478
7	38.86061631553295	56.79078193538894	3.528135144331129	0.8204666047469722
8	88.88388218092199	3.1045676503315156	6.195525229210156	1.8160249395363381
9	84.07879127851908	4.694003292165933	7.850620269810471	3.37658515950452
10	47.343920988017615	24.81281553755184	14.614274022235914	13.228989452194623
11	40.92015118214506	22.762292745280156	20.573646119750236	15.74390995282455
12	37.367554693175656	21.598079876405837	25.706482256329146	15.327883174089365
13	23.584046789218615	33.709020350735216	26.01307670378783	16.69385615625833
14	17.70467984146106	37.53271474945514	28.280808879652025	16.48179652943178
15	16.62672539841645	24.007246443449233	42.06376502395556	17.30226313417875
16	18.746769907028497	18.758356859751892	41.89271953137214	20.602153701847474
17	19.050237716450713	19.08886089219536	29.282804410398832	32.578096980955095
18	21.767286170144285	24.024167072823076	32.67925291742917	21.52929383960347
19	28.888664097918948	24.946341373697617	25.298915792280884	20.86607873610255
20	30.80418969496887	23.698444957376566	24.315496169868407	21.181869177786155
21	24.291770504768124	26.841084023799233	27.935959096217687	20.931186375214956
22	23.74093045069568	23.414472655711172	25.801384916730274	27.043211976862878
23	21.558353181354203	28.95395565691585	25.158952760177666	24.328738401552283
24	21.56092805973718	24.831023606117174	34.52139448056427	19.08665385358138
25	21.138280165160054	24.257193566482442	32.67318356124073	21.93134270711678
26	19.08003273773944	30.7140689515647	27.167909658552738	23.037988652143127
27	20.003494477805468	29.936639599790333	28.578943012423792	21.480922909980414
28	18.51190421452415	27.476895064509897	34.54438446612655	19.466816254839394
29	18.759644298943382	25.492767350542106	34.24073273681984	21.506855613694675
30	20.94148588874686	27.717646193318192	31.064252411649484	20.276615506285463
31	28.393000009195994	25.84387041004938	25.118306465703537	20.644823115051082
32	28.54933191101956	26.132072869058238	26.06310291237137	19.25549230755083
33	27.82340012690472	25.65903092612858	25.293582115630432	21.22398683133627
34	21.22361899156727	26.4193557286446	27.807399096953368	24.549626182834757
35	21.7437444249285	24.988642947132227	30.11651324682968	23.15109938110959
36	28.966830048830726	25.521091012754844	25.368621428505744	20.143457509908682
37	22.126849544338487	30.99951261230608	26.334200822121883	20.53943702123355
38	22.278031689396098	31.215434556707102	24.31310521136993	22.193428542526874
39	22.14064353567586	28.658948162180554	25.384806378341594	23.815601923801992
40	24.36202790064648	24.841507039533578	27.180784050467615	23.615681009352326
41	20.05352068638901	24.390535482743715	27.30695309123346	28.24899073963382
42	22.399602733049484	27.079628113993543	24.00779820310273	26.512970949854243
43	22.086203249864358	26.073770265672273	25.499572386268543	26.340454098194826
44	21.30491158051553	27.867724819068812	28.690030622660768	22.13733297775489
45	20.40958958277774	32.07434041731422	24.80325170355793	22.71281829635011
46	22.301573434611882	30.33979198661063	27.048361733628834	20.310272845148653
47	22.62766338982739	27.41767286170144	26.803932207130575	23.15073154134059
48	22.38470522240512	26.053171238608464	28.590529965147184	22.971593573839236
49	21.63284073457602	25.200702573958782	31.099748949357657	22.06670774210754
50	21.176351581251208	29.743523721067106	27.20763635360437	21.872488344077322
51	21.068942368704192	31.031146832439788	25.7112641733261	22.18864662552992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	264.0
1	554.0
2	844.0
3	2473.5
4	4103.0
5	3088.5
6	2074.0
7	2068.5
8	2063.0
9	2102.5
10	2142.0
11	2123.0
12	2104.0
13	2037.5
14	1971.0
15	1833.0
16	1695.0
17	1753.5
18	1812.0
19	1745.0
20	1678.0
21	1893.0
22	2108.0
23	2349.0
24	2590.0
25	3083.0
26	4384.5
27	5193.0
28	6298.0
29	7403.0
30	8461.5
31	9520.0
32	10937.0
33	12354.0
34	13434.5
35	14515.0
36	16607.5
37	18700.0
38	22815.0
39	26930.0
40	32776.5
41	38623.0
42	41430.5
43	44238.0
44	44594.5
45	44951.0
46	44480.5
47	44010.0
48	42429.0
49	40848.0
50	39211.5
51	37575.0
52	34878.0
53	32181.0
54	30239.5
55	28298.0
56	27049.5
57	25801.0
58	24113.5
59	22426.0
60	20246.5
61	18067.0
62	15650.0
63	13233.0
64	11340.0
65	9447.0
66	7961.0
67	6475.0
68	5806.5
69	5138.0
70	4446.5
71	3755.0
72	3108.5
73	2462.0
74	1863.5
75	975.0
76	685.0
77	483.5
78	282.0
79	207.0
80	132.0
81	113.0
82	94.0
83	68.5
84	43.0
85	35.0
86	27.0
87	17.5
88	8.0
89	7.0
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	543715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.32419311185335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.06657736522426	22.34939575469786
2	8.946249734471865	5.962531075568143
3	4.757024281816542	4.755719874150897
4	3.3336436319239127	4.443639366253304
5	2.479178211177284	4.130830673398548
6	1.8759728910816298	3.750916973700364
7	1.5601282015895117	3.639300942831215
8	1.323527474899464	3.5284388129914706
9	1.1386243726023282	3.4149364627016565
>10	7.428646222164144	40.88703835904959
>50	0.07014990588049995	1.540865795331056
>100	0.01863356874949813	1.0645616594738652
>500	5.480461396911216E-4	0.14665345900797971
>1k	0.0010960922793822432	0.38517079084412104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1096	0.20157619341015054	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.1863108429967906	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	803	0.14768766725214497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00864423457142069	0.0
2	0.0	0.0	0.0	0.03770357632215407	0.0
3	0.0	0.0	0.0	0.04634781089357476	0.0
4	0.0	0.0	0.0	0.06639507830389083	0.0
5	0.0	0.0	0.0	0.10593785347102802	0.0
6	0.0	0.0	0.0	0.16405653697249478	0.0
7	0.0	0.0	0.0	0.19992091444966573	0.0
8	0.0	0.0	0.0	0.4428790818719366	0.0
9	0.0	0.0	0.0	0.5493686949964596	0.0
10	0.0	0.0	0.0	0.7314493806497889	0.0
11	0.0	0.0	0.0	0.8749068905584727	0.0
12	0.0	0.0	0.0	1.0126628840477088	0.0
13	0.0	0.0	0.0	1.0733564459321518	0.0
14	0.0	0.0	0.0	1.0965303513789393	0.0
15	0.0	0.0	0.0	1.1320268890871137	0.0
16	0.0	0.0	0.0	1.2078018815004183	0.0
17	0.0	0.0	0.0	1.2925889482541404	0.0
18	0.0	0.0	0.0	1.450024369384696	0.0
19	0.0	0.0	0.0	1.5116375306916308	0.0
20	0.0	0.0	0.0	1.571227573269084	0.0
21	0.0	0.0	0.0	1.6510488031413517	0.0
22	0.0	0.0	0.0	1.735468030126077	0.0
23	0.0	0.0	0.0	1.8294510911047148	0.0
24	0.0	0.0	0.0	1.9092723209769824	0.0
25	0.0	0.0	0.0	1.9760352390498699	0.0
26	0.0	0.0	0.0	2.0413267980467706	0.0
27	0.0	0.0	0.0	2.1068022769281702	0.0
28	0.0	0.0	0.0	2.1739330347700543	0.0
29	0.0	0.0	0.0	2.246213549377891	0.0
30	0.0	0.0	0.0	2.3458981267759764	0.0
31	0.0	0.0	0.0	2.4321565526056848	0.0
32	0.0	0.0	0.0	2.5097707438639727	0.0
33	0.0	0.0	0.0	2.5940060509642	0.0
34	0.0	0.0	0.0	2.6844946341373697	0.0
35	0.0	0.0	0.0	2.8102958351342155	0.0
36	0.0	0.0	0.0	2.9009683381918836	0.0
37	0.0	0.0	0.0	2.997158437784501	0.0
38	0.0	0.0	0.0	3.0959234157600948	0.0
39	0.0	0.0	0.0	3.222460296294934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGG	50	2.1827873E-11	45.000004	3
ATAACGG	25	3.8880837E-5	45.000004	2
CGGGTAC	20	7.0300046E-4	45.0	6
ATCTCGT	45	3.8380676E-10	45.0	19
CGAACTA	20	7.0300046E-4	45.0	38
TACGGGA	55	1.8189894E-12	45.0	4
GCGCATA	20	7.0300046E-4	45.0	1
TACGCAT	20	7.0300046E-4	45.0	45
TAGTGCG	20	7.0300046E-4	45.0	1
CGTTAAG	30	2.1633841E-6	44.999996	2
AGGCGAT	360	0.0	44.375	7
CGACGGT	120	0.0	43.124996	28
GACGGTC	120	0.0	43.124996	29
CGGTCTA	115	0.0	43.043476	31
CGTTTTA	650	0.0	42.923073	1
CGTTTTT	855	0.0	42.631577	1
TCACGAC	130	0.0	41.53846	25
ACGGTCT	125	0.0	41.4	30
GCGCGTC	50	1.0786607E-9	40.500004	38
TGCGCGT	50	1.0786607E-9	40.500004	37
>>END_MODULE