FastQCFastQC Report
Thu 26 May 2016
SRR1545754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545754_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences770632
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.3364770733631617No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17270.22410177620446592No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.1544187108762678No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.1384577852983006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.8548654E-444.01
GATACCC207.8548654E-444.013
TCACACG207.8548654E-444.01
GAGTACG254.4414708E-544.01
GCACGAC207.8548654E-444.042
TTCGTAA408.305506E-944.01
CGTCTAT207.8548654E-444.019
CAATTCG351.4452598E-744.043
TGCGCAC207.8548654E-444.021
TCTACGA207.8548654E-444.026
TCTAACG408.305506E-944.01
ACGGACT302.5266272E-644.027
TCGTAAT207.8548654E-444.023
ACCCGTA207.8548654E-444.033
CAGTCGA207.8548654E-444.014
ACGTACT254.4414708E-544.028
TAGGCCG207.8548654E-444.06
CGGTCAT254.4414708E-544.027
CGCGTAG207.8548654E-444.02
CGCGTAA207.8548654E-444.031
Produced by FastQC (version 0.11.5)