##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545754_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	770632
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5636244017897	31.0	31.0	33.0	30.0	34.0
2	31.762986743348318	31.0	31.0	34.0	30.0	34.0
3	31.82608560246655	31.0	31.0	34.0	30.0	34.0
4	35.710519417828486	37.0	35.0	37.0	35.0	37.0
5	27.033961735302974	35.0	22.0	37.0	0.0	37.0
6	31.319745351867038	35.0	26.0	37.0	17.0	37.0
7	15.082267541446502	0.0	0.0	32.0	0.0	37.0
8	25.152962763030864	17.0	17.0	35.0	17.0	37.0
9	33.26730138379927	32.0	32.0	37.0	28.0	39.0
10	36.110203054116624	37.0	35.0	39.0	32.0	39.0
11	37.08899967818622	39.0	37.0	39.0	34.0	39.0
12	37.00532160616221	39.0	35.0	39.0	33.0	39.0
13	36.48010464138525	39.0	35.0	39.0	33.0	39.0
14	37.80324720489157	40.0	36.0	41.0	33.0	41.0
15	38.08812377373377	40.0	36.0	41.0	33.0	41.0
16	38.159016236024456	40.0	36.0	41.0	34.0	41.0
17	38.184879423641895	40.0	36.0	41.0	34.0	41.0
18	38.17163834359331	40.0	36.0	41.0	34.0	41.0
19	38.11354965794309	40.0	36.0	41.0	34.0	41.0
20	38.12488710564835	40.0	36.0	41.0	34.0	41.0
21	37.98352521047659	40.0	36.0	41.0	34.0	41.0
22	37.90959757705364	40.0	35.0	41.0	34.0	41.0
23	37.8173187721247	40.0	35.0	41.0	34.0	41.0
24	37.69570560267417	40.0	35.0	41.0	33.0	41.0
25	37.5346559706838	39.0	35.0	41.0	33.0	41.0
26	37.2854488264178	39.0	35.0	40.0	33.0	41.0
27	37.05365596030271	39.0	35.0	40.0	33.0	41.0
28	37.11154610761038	39.0	35.0	40.0	33.0	41.0
29	37.071970797994375	39.0	35.0	40.0	33.0	41.0
30	36.89102191448058	38.0	35.0	40.0	32.0	41.0
31	36.67933202877638	38.0	35.0	40.0	32.0	41.0
32	36.438415482357335	38.0	35.0	40.0	31.0	41.0
33	36.24230631481693	38.0	35.0	40.0	31.0	41.0
34	36.0449059992318	38.0	35.0	40.0	30.0	41.0
35	35.89050934817137	38.0	35.0	40.0	30.0	41.0
36	35.71450835158675	38.0	35.0	40.0	30.0	41.0
37	35.564111015374394	38.0	35.0	40.0	29.0	41.0
38	35.41076155674823	38.0	34.0	40.0	29.0	41.0
39	35.371284867485386	38.0	34.0	40.0	29.0	41.0
40	35.16499833902563	38.0	34.0	40.0	27.0	41.0
41	34.965432268579555	38.0	34.0	40.0	27.0	41.0
42	34.97815688941025	38.0	34.0	40.0	27.0	41.0
43	34.865959368414494	37.0	34.0	40.0	27.0	41.0
44	34.65634570067166	37.0	34.0	40.0	26.0	40.0
45	34.43352469142211	37.0	34.0	40.0	26.0	40.0
46	34.42338755722576	37.0	34.0	40.0	26.0	40.0
47	34.274848436088824	36.0	33.0	39.0	25.0	40.0
48	34.204571053369186	36.0	33.0	39.0	25.0	40.0
49	34.08888678383457	36.0	33.0	39.0	24.0	40.0
50	34.13320106094738	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	9.0
16	34.0
17	94.0
18	221.0
19	469.0
20	896.0
21	1577.0
22	2634.0
23	3954.0
24	6052.0
25	8881.0
26	11367.0
27	12364.0
28	13012.0
29	14804.0
30	18244.0
31	24477.0
32	34602.0
33	65100.0
34	66584.0
35	80064.0
36	102098.0
37	191414.0
38	90184.0
39	21490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38843961839114	23.019158301238463	28.58562841927145	15.006773661098944
2	32.46504168007557	24.062587590445244	30.617337458086354	12.855033271392829
3	30.51274797828276	23.69042552087118	32.86159931069564	12.935227190150423
4	26.191619346198962	27.292014865720603	31.72136635904037	14.79499942904006
5	17.57855889711302	48.24663393163014	22.970756470014223	11.204050701242616
6	20.360301674469785	41.050722004796064	28.248372764172785	10.340603556561367
7	39.70377560236273	55.509763414963295	3.8781156245782684	0.9083453580956928
8	88.54550550716814	2.8662188956596664	7.232245741157907	1.3560298560142843
9	84.21970538467129	4.547176862627039	8.748274143819618	2.4848436088820605
10	51.32826044078107	23.5274424109043	13.992670950596395	11.151626197718237
11	46.630428012332736	19.826843422022446	19.826064840286932	13.716663725357888
12	41.37655846110725	20.513552512742788	24.682727942779433	13.427161083370532
13	22.30961081294314	38.20500576150484	24.66585867184337	14.819524753708643
14	16.41808282033448	40.71061155000052	28.54799696872178	14.323308660943226
15	15.250210217068588	22.019199825597692	47.93325478308713	14.797335174246593
16	16.894055787976622	17.48396121624848	46.10786990418254	19.514113091592357
17	17.873381847626362	16.803091488544467	30.274372203593934	35.049154460235236
18	21.78653365030261	21.50857997072533	35.02994944409264	21.674936934879423
19	29.24664431271995	23.63138307259496	25.94649586313571	21.175476751549375
20	31.575641810877308	22.490371539204183	24.918248917771386	21.015737732147123
21	23.279723655389343	27.317708062992452	28.840094883160834	20.56247339845737
22	23.022791682670846	23.24987802219477	26.76595313976061	26.96137715537377
23	20.548588690840763	29.13400948831608	25.76768159121345	24.549720229629706
24	20.343951458024062	22.65893448494223	38.89560776090274	18.101506296130967
25	18.857509161311757	23.453347382408204	36.28748871056484	21.401654745715206
26	17.931386186921902	32.56781446916297	28.011164862087224	21.489634481827903
27	18.854784125237465	31.32000747438466	29.883005117877275	19.942203282500596
28	16.141426776983046	26.395737524525327	39.29527971846484	18.167555980026783
29	17.10531096554516	23.77918383871939	37.54373034081118	21.571774854924268
30	19.710834743431366	28.463261323173704	31.922759501292447	19.903144432102483
31	29.66357483208587	24.761624225311174	25.63207860561202	19.942722336990936
32	30.86998723125954	25.236169793104878	26.217831597961155	17.676011377674428
33	27.936421015478203	25.880446179239897	26.247417703910557	19.935715101371343
34	20.103758992619046	26.452054936727258	29.42156567596466	24.022620394689035
35	21.248403907442203	24.695055486925018	31.806102004588443	22.250438601044337
36	29.91388886005253	25.16869270936063	25.64388709526726	19.273531335319582
37	21.816249519874596	31.90679857571448	26.82291937007547	19.454032534335454
38	21.885413530712455	32.29751684331821	23.703920937620033	22.113148688349302
39	21.491191645298922	30.33510157896376	25.95519002584891	22.218516749888405
40	24.50728752504438	25.21346115915249	25.720836923460226	24.558414392342907
41	18.321844927280466	24.49080754497607	26.73078719804005	30.456560329703414
42	22.594961019007776	25.32285189299173	24.07309844387464	28.009088644125864
43	22.101729489561812	26.140881769768193	25.517627090491963	26.239761650178036
44	20.648895971099044	28.982186049891517	28.658685338786867	21.71023264022257
45	18.926154117659273	34.94287805333804	23.137372961413487	22.9935948675892
46	21.69037880596705	31.633646150172844	26.29685764411548	20.379117399744626
47	22.97607677854021	26.76647219425095	26.693934329225883	23.56351669798295
48	22.495691847730175	23.845622813482958	30.76345129711717	22.895234041669692
49	21.582285708353663	23.530946028714094	32.34539962005211	22.54136864288013
50	20.349531293795224	31.53165194282096	26.621396464200814	21.49742029918301
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	747.5
2	1089.0
3	4960.0
4	8831.0
5	6516.5
6	4202.0
7	4298.5
8	4395.0
9	4652.5
10	4910.0
11	4750.0
12	4590.0
13	4337.5
14	4085.0
15	3814.0
16	3543.0
17	3326.0
18	3109.0
19	3013.5
20	2918.0
21	2938.0
22	2958.0
23	3036.0
24	3114.0
25	3750.5
26	4387.0
27	5506.0
28	6625.0
29	8144.0
30	9663.0
31	10885.5
32	12108.0
33	14021.5
34	15935.0
35	17082.5
36	18230.0
37	22311.0
38	26392.0
39	36270.5
40	46149.0
41	57703.0
42	69257.0
43	72566.0
44	75875.0
45	72721.5
46	69568.0
47	67840.5
48	66113.0
49	62422.0
50	58731.0
51	53617.0
52	48503.0
53	44538.0
54	40573.0
55	38078.0
56	35583.0
57	33971.5
58	32360.0
59	29238.0
60	26116.0
61	23205.0
62	20294.0
63	17042.0
64	13790.0
65	11402.0
66	9014.0
67	7697.5
68	6381.0
69	5401.0
70	4421.0
71	3594.5
72	2768.0
73	2246.5
74	1725.0
75	1357.0
76	989.0
77	731.0
78	473.0
79	352.5
80	232.0
81	172.0
82	112.0
83	83.0
84	54.0
85	52.0
86	50.0
87	28.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	770632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.51014970667146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.18488139598925	16.957318004540056
2	7.131222753053105	3.495746745379068
3	3.5231875291689696	2.5906156135382807
4	2.3860032635445867	2.3392518876033797
5	1.8812546640847738	2.305491672654587
6	1.5889432087716906	2.3367141553435835
7	1.3087337020372167	2.2454081274168978
8	1.1071968211510654	2.1710047872930684
9	0.9906268897071366	2.185237203315861
>10	10.319808662398856	52.90121993398215
>50	0.5329480013921288	8.0419634675738
>100	0.04298856681340654	1.5415800831792656
>500	0.0	0.0
>1k	0.0022045418878670025	0.8884483181799886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2593	0.3364770733631617	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1727	0.22410177620446592	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1190	0.1544187108762678	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1067	0.1384577852983006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01141919878748871	0.0
2	0.0	0.0	0.0	0.034906414475391626	0.0
3	0.0	0.0	0.0	0.04489821341444425	0.0
4	0.0	0.0	0.0	0.05748528480519885	0.0
5	0.0	0.0	0.0	0.08733091799977162	0.0
6	0.0	0.0	0.0	0.14416738469204496	0.0
7	0.0	0.0	0.0	0.1797226172803621	0.0
8	0.0	0.0	0.0	0.374887105648351	0.0
9	0.0	0.0	0.0	0.4505392976154637	0.0
10	0.0	0.0	0.0	0.6049580084917314	0.0
11	0.0	0.0	0.0	0.7666434822327648	0.0
12	0.0	0.0	0.0	0.9231384110703942	0.0
13	0.0	0.0	0.0	0.9843868409305608	0.0
14	0.0	0.0	0.0	1.006835947637783	0.0
15	0.0	0.0	0.0	1.047451961506919	0.0
16	0.0	0.0	0.0	1.1368590974680521	0.0
17	0.0	0.0	0.0	1.247806994778312	0.0
18	0.0	0.0	0.0	1.4076757778031537	0.0
19	0.0	0.0	0.0	1.4754123887925754	0.0
20	0.0	0.0	0.0	1.5432787634045821	0.0
21	0.0	0.0	0.0	1.632166844875375	0.0
22	0.0	0.0	0.0	1.7194977628751467	0.0
23	0.0	0.0	0.0	1.8220110247173749	0.0
24	0.0	0.0	0.0	1.9103800516978273	0.0
25	0.0	0.0	0.0	1.9694224999740473	0.0
26	0.0	0.0	0.0	2.042349655866873	0.0
27	0.0	0.0	0.0	2.1203375930405173	0.0
28	0.0	0.0	0.0	2.190539712859056	0.0
29	0.0	0.0	0.0	2.2751455947845405	0.0
30	0.0	0.0	0.0	2.3711706754975137	0.0
31	0.0	0.0	0.0	2.4666767017201465	0.0
32	0.0	0.0	0.0	2.550114711042365	0.0
33	0.0	0.0	0.0	2.640170665116424	0.0
34	0.0	0.0	0.0	2.7371040911874926	0.0
35	0.0	0.0	0.0	2.8585628419271454	0.0
36	0.0	0.0	0.0	2.9596487039209376	0.0
37	0.0	0.0	0.0	3.0576202389726874	0.0
38	0.0	0.0	0.0	3.1555917740244372	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.8548654E-4	44.0	1
GATACCC	20	7.8548654E-4	44.0	13
TCACACG	20	7.8548654E-4	44.0	1
GAGTACG	25	4.4414708E-5	44.0	1
GCACGAC	20	7.8548654E-4	44.0	42
TTCGTAA	40	8.305506E-9	44.0	1
CGTCTAT	20	7.8548654E-4	44.0	19
CAATTCG	35	1.4452598E-7	44.0	43
TGCGCAC	20	7.8548654E-4	44.0	21
TCTACGA	20	7.8548654E-4	44.0	26
TCTAACG	40	8.305506E-9	44.0	1
ACGGACT	30	2.5266272E-6	44.0	27
TCGTAAT	20	7.8548654E-4	44.0	23
ACCCGTA	20	7.8548654E-4	44.0	33
CAGTCGA	20	7.8548654E-4	44.0	14
ACGTACT	25	4.4414708E-5	44.0	28
TAGGCCG	20	7.8548654E-4	44.0	6
CGGTCAT	25	4.4414708E-5	44.0	27
CGCGTAG	20	7.8548654E-4	44.0	2
CGCGTAA	20	7.8548654E-4	44.0	31
>>END_MODULE