FastQCFastQC Report
Thu 26 May 2016
SRR1545753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545753_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences298322
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.2477189077573897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6960.23330495236690554No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.22827682839348085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC950.045.00000416
CGACAAC253.8838378E-545.00000435
TAGCATA950.045.00000430
GCCGATG351.2078817E-745.0000049
CATAGGA700.045.0000044
CGCTAGA253.8838378E-545.00000424
ATTGCGG253.8838378E-545.0000042
GATTACC253.8838378E-545.0000049
CCGCAAT253.8838378E-545.00000433
CGGACGA253.8838378E-545.00000431
GTGCAAC207.02488E-445.041
ATAGGCG406.7866495E-945.05
GTTTCGT207.02488E-445.043
TGCCACG207.02488E-445.044
GCGAATA207.02488E-445.01
TAGGTGC207.02488E-445.06
CGATTTG207.02488E-445.010
CTAACGC207.02488E-445.029
CATAGGC551.8189894E-1245.04
CGCTAAC207.02488E-445.027