FastQCFastQC Report
Thu 26 May 2016
SRR1545752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545752_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1022280
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.4157373713659662No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31620.30930860429627893No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.1715772586766835No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.16179520287983723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.856074E-444.033
AACCGTC302.5274421E-644.020
AACGTAC302.5274421E-644.025
AGCGTGC254.442494E-544.031
AAGTACG408.310963E-944.01
CTGTCGT254.442494E-544.011
GGTACGA207.856074E-444.01
ACACGGC207.856074E-444.041
CTACTCG207.856074E-444.037
CGCGGTA254.442494E-544.030
CAACGGC207.856074E-444.03
GCGAATA302.5274421E-644.01
ACACCCG408.310963E-944.029
GACCGTA207.856074E-444.021
CTCCCGG207.856074E-444.015
ATAGCGG600.044.02
CGACCGT302.5274421E-644.044
GCACGGT351.4458783E-744.040
GTCTACT207.856074E-444.035
TAAGTAC302.5274421E-644.034
Produced by FastQC (version 0.11.5)