FastQCFastQC Report
Thu 26 May 2016
SRR1545751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545751_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences400158
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13510.33761664142663644No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.31162690737158827No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.250900894146812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC207.0277683E-445.00000416
AGGTATG453.8380676E-1045.0000047
CGGGTGG207.0277683E-445.0000046
TTAGGCG406.7957444E-945.0000045
TCGCAAG207.0277683E-445.0000042
GTCGAAT207.0277683E-445.00000437
TTGTGCA207.0277683E-445.0000041
ATAGGCG406.7957444E-945.0000045
CACGGGC207.0277683E-445.0000044
CACGGGA351.2092823E-745.0000044
TCCGATT207.0277683E-445.00000435
GAGTAAT207.0277683E-445.00000419
GGTCCAC207.0277683E-445.00000421
TAAACGG207.0277683E-445.0000042
CGCGCAT207.0277683E-445.00000442
TTACTAC302.1619417E-645.00000427
CGATTGC207.0277683E-445.00000410
AGTGGCC207.0277683E-445.00000415
GCGCATT207.0277683E-445.00000443
TGTGCGG406.7957444E-945.0000042