##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545751_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	400158
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02977074055748	31.0	31.0	33.0	30.0	33.0
2	31.169960365655566	31.0	31.0	33.0	30.0	34.0
3	31.08494894516666	31.0	31.0	34.0	28.0	34.0
4	35.035126125180554	35.0	35.0	37.0	32.0	37.0
5	32.752240365055805	35.0	35.0	37.0	28.0	37.0
6	33.94888269133692	35.0	35.0	37.0	28.0	37.0
7	15.732992967777728	0.0	0.0	35.0	0.0	37.0
8	25.15370928483249	17.0	17.0	35.0	17.0	37.0
9	32.89207513032352	32.0	32.0	37.0	27.0	39.0
10	35.66793366620185	37.0	35.0	38.0	32.0	39.0
11	36.5518295273367	37.0	35.0	39.0	32.0	39.0
12	36.094535158612345	37.0	35.0	39.0	32.0	39.0
13	35.46023570689577	37.0	35.0	39.0	30.0	39.0
14	36.43216929312921	38.0	35.0	40.0	31.0	41.0
15	36.984743526307106	39.0	35.0	41.0	32.0	41.0
16	37.16865837993993	39.0	35.0	41.0	32.0	41.0
17	37.15985435752878	39.0	35.0	41.0	32.0	41.0
18	37.06057357343849	38.0	35.0	40.0	32.0	41.0
19	36.95877628336807	38.0	35.0	40.0	32.0	41.0
20	36.99595659714413	38.0	35.0	40.0	32.0	41.0
21	36.76989839013589	38.0	35.0	40.0	31.0	41.0
22	36.83129413881517	38.0	35.0	40.0	32.0	41.0
23	36.836776973095624	38.0	35.0	40.0	32.0	41.0
24	36.65374177200006	38.0	35.0	40.0	32.0	41.0
25	36.397542970526644	38.0	35.0	40.0	31.0	41.0
26	36.23794351231264	38.0	35.0	40.0	31.0	41.0
27	36.09719160931432	38.0	34.0	40.0	31.0	41.0
28	36.07233642711129	38.0	34.0	40.0	31.0	41.0
29	36.16364536008277	38.0	35.0	40.0	31.0	41.0
30	35.918192314035956	37.0	34.0	40.0	30.0	41.0
31	35.7256208797525	37.0	34.0	40.0	30.0	41.0
32	35.495471788643485	37.0	34.0	40.0	29.0	41.0
33	35.243988624493326	37.0	34.0	40.0	27.0	41.0
34	35.05338641236712	37.0	34.0	40.0	26.0	41.0
35	34.85635673908806	37.0	34.0	40.0	25.0	41.0
36	34.628259337561666	37.0	33.0	40.0	24.0	41.0
37	34.44341735014669	37.0	33.0	40.0	23.0	41.0
38	34.39117798469604	37.0	33.0	40.0	23.0	41.0
39	34.3087755336642	37.0	33.0	40.0	23.0	41.0
40	34.1504380769596	36.0	33.0	40.0	23.0	41.0
41	34.07304614677202	36.0	33.0	40.0	23.0	41.0
42	34.020114554750876	36.0	33.0	40.0	22.0	41.0
43	33.88915628326811	36.0	33.0	40.0	22.0	41.0
44	33.56757080953024	36.0	33.0	40.0	20.0	41.0
45	33.52399302275601	36.0	33.0	40.0	20.0	41.0
46	33.55927158772285	36.0	33.0	40.0	20.0	41.0
47	33.55416360537588	36.0	33.0	40.0	20.0	41.0
48	33.5724588787429	36.0	33.0	40.0	20.0	41.0
49	33.51804287306514	36.0	33.0	40.0	20.0	41.0
50	33.34643065988934	35.0	33.0	40.0	20.0	41.0
51	33.0711219068468	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	8.0
15	21.0
16	50.0
17	167.0
18	304.0
19	582.0
20	1017.0
21	1663.0
22	2680.0
23	3888.0
24	5486.0
25	7201.0
26	8126.0
27	8469.0
28	8945.0
29	9902.0
30	12018.0
31	15876.0
32	21130.0
33	33965.0
34	43012.0
35	40587.0
36	40061.0
37	62886.0
38	61607.0
39	10504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.188575512672493	24.20568875294259	29.39189020336967	16.21384553101525
2	32.227020326970845	25.002374062245416	30.04113375216789	12.729471858615845
3	29.38414326341095	22.616066653671798	34.371173386512325	13.628616696404919
4	24.622774004268315	28.50923885065399	31.94913009361302	14.91885705146467
5	20.92598423622669	36.099990503751016	30.671634704291804	12.302390555730486
6	19.83966333298347	40.23135861334773	29.526337096846746	10.402640956822056
7	38.066463746820006	56.03461632655101	5.259922330679381	0.6389975959495999
8	86.90242354270063	3.1557534773764364	8.314465786014525	1.6273571939084062
9	83.45378575462692	4.3582784800004	9.957816662418345	2.2301191029543332
10	60.20122051789543	17.67801718321263	13.871270848015035	8.249491450876903
11	56.842047391280445	16.87358493395109	18.07785924559799	8.206508429170478
12	48.057267379385145	21.85036910420384	20.244003618570662	9.848359897840353
13	19.20291484863479	50.30762848674774	21.334073041148745	9.15538362346873
14	12.128459258592855	50.08646584599084	27.3429495349337	10.44212536048261
15	10.072271452776153	21.204124370873505	58.27523128364296	10.448372892707381
16	10.842467225445949	16.027419169428075	55.72248961660144	17.40762398852453
17	12.583529505845192	15.757275876028967	30.931032242264305	40.72816237586154
18	19.64124170952474	21.203874469584512	39.08006337496689	20.074820445923862
19	30.3522608569615	21.843621769401086	27.35644420453921	20.447673169098206
20	33.26411067628287	21.189130293534053	25.750578521484012	19.796180508699063
21	19.197666921565983	30.571424287406472	30.03688543025505	20.194023360772494
22	21.053683794901016	24.70124300901144	26.425811804337286	27.81926139175026
23	17.153974180198823	32.29599308273232	25.815053054043652	24.734979683025205
24	16.97679416630431	22.714027958956212	45.37482694335737	14.934350931382104
25	13.476676712698485	24.233927598598555	43.67324906661869	18.616146622084276
26	12.354120122551592	38.32311237061361	30.339265989933978	18.983501516900823
27	14.848634789258245	38.486547813613626	30.081617760984415	16.58319963614372
28	11.276295863134061	29.979907936365137	44.47268329010041	14.27111291040039
29	12.051489661583673	23.99127344698844	43.43334382918747	20.523893062240415
30	15.640821875359235	32.677592351021346	33.54699893542051	18.134586838198913
31	30.374502071681686	26.47304314795656	25.096586848194963	18.055867932166795
32	31.916143123466227	26.40307078703912	27.440660938929124	14.240125150565527
33	29.73200585768621	26.675463192039146	25.681855667011533	17.91067528326311
34	17.36888928873095	28.639187520929234	29.440121152144904	24.551802038194914
35	18.120592366015423	25.06359987804817	33.96158517385633	22.854222582080077
36	32.53989674078739	23.111371008451663	26.34709289830517	18.001639352455783
37	19.310872205478834	33.44728832111316	30.040134147011933	17.201705326396073
38	19.566771125405467	35.00292384508119	23.332283747919572	22.098021281593773
39	19.133192389006343	33.08818016883331	28.329060021291593	19.449567420868757
40	24.11572428890588	25.69734954692896	24.46233737673619	25.724588787428964
41	16.26357588752443	22.62181438331859	26.767926668965757	34.346683060191225
42	22.031797440011193	24.082487417470098	23.922800493804946	29.962914648713756
43	21.05243428845606	26.44705341390151	26.467045517020775	26.033466780621655
44	18.472453380914537	32.310237456204796	29.15298457109442	20.064324591786246
45	14.45603986425362	42.361017398127736	22.101769800928633	21.081172936690006
46	21.518500192423993	34.83573988274632	25.361732115814256	18.284027809015438
47	21.254104628671673	27.6243383863374	26.578501491910693	24.54305549308023
48	22.71052934091034	22.929442870066325	32.94198791477366	21.418039874249672
49	21.186631280644143	22.01005602786899	34.79500597264081	22.008306718846054
50	19.440071171887105	35.48898185216839	25.14856631630506	19.922380659639444
51	17.09474757470799	36.71324826693456	23.150855412112215	23.041148746245234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	459.0
1	492.5
2	526.0
3	2209.5
4	3893.0
5	3047.5
6	2202.0
7	2428.0
8	2654.0
9	2827.0
10	3000.0
11	3069.0
12	3138.0
13	3088.0
14	3038.0
15	2831.5
16	2625.0
17	2543.0
18	2461.0
19	2348.5
20	2236.0
21	2186.0
22	2136.0
23	2032.5
24	1929.0
25	2276.0
26	3086.0
27	3549.0
28	4214.5
29	4880.0
30	5332.0
31	5784.0
32	6290.0
33	6796.0
34	7568.0
35	8340.0
36	10290.0
37	12240.0
38	18059.5
39	23879.0
40	32871.5
41	41864.0
42	46532.5
43	51201.0
44	48649.0
45	46097.0
46	41816.5
47	37536.0
48	34018.5
49	30501.0
50	28600.0
51	26699.0
52	23369.0
53	20039.0
54	17026.0
55	14013.0
56	12177.5
57	10342.0
58	8920.5
59	7499.0
60	6469.5
61	5440.0
62	4508.5
63	3577.0
64	2898.5
65	2220.0
66	1785.5
67	1351.0
68	1245.0
69	1139.0
70	1026.0
71	913.0
72	773.5
73	634.0
74	488.5
75	252.0
76	161.0
77	127.5
78	94.0
79	80.0
80	66.0
81	45.0
82	24.0
83	18.5
84	13.0
85	7.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.797270240090548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72342997591255	18.50268705634827
2	6.979796776652419	3.6011940733643075
3	3.301251176595698	2.5548980619916835
4	2.259759455848502	2.3318250144049513
5	1.6399478947857402	2.115308951072766
6	1.2983310186990404	2.009603769028272
7	1.0546395436892964	1.9044774920106995
8	0.8788657682317579	1.8137870182271556
9	0.7869842860094033	1.827184167080036
>10	9.612593483396259	54.517949966327436
>50	0.43943284490087897	7.133798901403968
>100	0.021971642245043947	0.7592656059242837
>500	0.0	0.0
>1k	0.002996133033415084	0.9280199228161758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1351	0.33761664142663644	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1247	0.31162690737158827	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1004	0.250900894146812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013994472183487523	0.0
2	0.0	0.0	0.0	0.054978283577986696	0.0
3	0.0	0.0	0.0	0.07522028798624543	0.0
4	0.0	0.0	0.0	0.11620409938074461	0.0
5	0.0	0.0	0.0	0.20242004408258737	0.0
6	0.0	0.0	0.0	0.2946336197202105	0.0
7	0.0	0.0	0.0	0.35535963294498674	0.0
8	0.0	0.0	0.0	0.8341705026514526	0.0
9	0.0	0.0	0.0	1.0313426196652322	0.0
10	0.0	0.0	0.0	1.3574638017982896	0.0
11	0.0	0.0	0.0	1.587872790247852	0.0
12	0.0	0.0	0.0	1.827528126390076	0.0
13	0.0	0.0	0.0	1.9227405174955892	0.0
14	0.0	0.0	0.0	1.9489801528396284	0.0
15	0.0	0.0	0.0	2.0002099170827523	0.0
16	0.0	0.0	0.0	2.1239110551332225	0.0
17	0.0	0.0	0.0	2.2693536053258963	0.0
18	0.0	0.0	0.0	2.5310002548993147	0.0
19	0.0	0.0	0.0	2.608469654486478	0.0
20	0.0	0.0	0.0	2.6969347107892383	0.0
21	0.0	0.0	0.0	2.791397398027779	0.0
22	0.0	0.0	0.0	2.8831111710874207	0.0
23	0.0	0.0	0.0	2.995816652422293	0.0
24	0.0	0.0	0.0	3.073785854587438	0.0
25	0.0	0.0	0.0	3.1322627562112966	0.0
26	0.0	0.0	0.0	3.1952378810369906	0.0
27	0.0	0.0	0.0	3.25996231488562	0.0
28	0.0	0.0	0.0	3.3189390190874604	0.0
29	0.0	0.0	0.0	3.391660294183797	0.0
30	0.0	0.0	0.0	3.5036160716516975	0.0
31	0.0	0.0	0.0	3.6048260936929912	0.0
32	0.0	0.0	0.0	3.691541840972816	0.0
33	0.0	0.0	0.0	3.771760154738878	0.0
34	0.0	0.0	0.0	3.864473532954483	0.0
35	0.0	0.0	0.0	3.9989204264315594	0.0
36	0.0	0.0	0.0	4.090884100780192	0.0
37	0.0	0.0	0.0	4.192843826688458	0.0
38	0.0	0.0	0.0	4.283557994592136	0.0
39	0.0	0.0	0.0	4.374022261206823	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAC	20	7.0277683E-4	45.000004	16
AGGTATG	45	3.8380676E-10	45.000004	7
CGGGTGG	20	7.0277683E-4	45.000004	6
TTAGGCG	40	6.7957444E-9	45.000004	5
TCGCAAG	20	7.0277683E-4	45.000004	2
GTCGAAT	20	7.0277683E-4	45.000004	37
TTGTGCA	20	7.0277683E-4	45.000004	1
ATAGGCG	40	6.7957444E-9	45.000004	5
CACGGGC	20	7.0277683E-4	45.000004	4
CACGGGA	35	1.2092823E-7	45.000004	4
TCCGATT	20	7.0277683E-4	45.000004	35
GAGTAAT	20	7.0277683E-4	45.000004	19
GGTCCAC	20	7.0277683E-4	45.000004	21
TAAACGG	20	7.0277683E-4	45.000004	2
CGCGCAT	20	7.0277683E-4	45.000004	42
TTACTAC	30	2.1619417E-6	45.000004	27
CGATTGC	20	7.0277683E-4	45.000004	10
AGTGGCC	20	7.0277683E-4	45.000004	15
GCGCATT	20	7.0277683E-4	45.000004	43
TGTGCGG	40	6.7957444E-9	45.000004	2
>>END_MODULE