FastQCFastQC Report
Thu 26 May 2016
SRR1545750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545750_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1080825
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.46159183956699745No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.20512108805773366No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.19929220734161407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA700.044.01
ACGCTAA254.4426626E-544.013
ACGTATC302.5275767E-644.021
TATACGC207.856274E-444.022
CGTTTTA26450.043.5009461
CGGTCTA2000.042.931
CGTTATT15300.041.5555531
AGGCGAT7650.041.1241847
CGTTAAG650.040.6153832
GTTTTAT30900.040.0841452
TCGCCGT557.8216544E-1140.00000425
TCACGAC2250.039.1111125
CGTTCGA452.35068E-839.1111114
TTACTGG15950.039.0344840
ACATACG2650.039.0188717
CGTAAAG1250.038.722
TTACGGG2850.038.5964933
CGTTTTT13250.038.5207561
ATATGCT5350.038.2429934
GTTATTT18900.038.1798932
Produced by FastQC (version 0.11.5)