##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545750_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1080825
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49146624106585	31.0	31.0	33.0	30.0	34.0
2	31.679720583813292	31.0	31.0	34.0	30.0	34.0
3	31.73341012652372	31.0	31.0	34.0	30.0	34.0
4	35.648975551083666	37.0	35.0	37.0	33.0	37.0
5	27.091226609303078	35.0	25.0	37.0	0.0	37.0
6	31.32580019892212	35.0	26.0	37.0	17.0	37.0
7	15.067383711516666	0.0	0.0	32.0	0.0	37.0
8	25.121512733328707	17.0	17.0	35.0	17.0	37.0
9	33.15532116670136	32.0	32.0	37.0	28.0	39.0
10	35.95268059121504	37.0	35.0	39.0	32.0	39.0
11	36.943482062313514	38.0	37.0	39.0	34.0	39.0
12	37.08877107764902	39.0	37.0	39.0	34.0	39.0
13	36.71873059931071	39.0	35.0	39.0	33.0	39.0
14	38.01682418522888	40.0	37.0	41.0	33.0	41.0
15	38.244620544491475	40.0	37.0	41.0	33.0	41.0
16	38.296060879420814	40.0	37.0	41.0	34.0	41.0
17	38.312230934702654	40.0	37.0	41.0	34.0	41.0
18	38.28960655055166	40.0	37.0	41.0	34.0	41.0
19	38.23627414243749	40.0	37.0	41.0	34.0	41.0
20	38.26673698332292	40.0	37.0	41.0	34.0	41.0
21	38.14722457382092	40.0	37.0	41.0	34.0	41.0
22	38.06895195799505	40.0	36.0	41.0	34.0	41.0
23	37.96378322115051	40.0	36.0	41.0	34.0	41.0
24	37.8211495848079	40.0	35.0	41.0	33.0	41.0
25	37.63606041681123	39.0	35.0	41.0	33.0	41.0
26	37.385353780676795	39.0	35.0	40.0	33.0	41.0
27	37.21362940346494	39.0	35.0	40.0	33.0	41.0
28	37.224843059699765	39.0	35.0	40.0	33.0	41.0
29	37.1196151088289	39.0	35.0	40.0	32.0	41.0
30	36.89449170772327	39.0	35.0	40.0	32.0	41.0
31	36.726009298452574	39.0	35.0	40.0	31.0	41.0
32	36.533838502995394	39.0	35.0	40.0	31.0	41.0
33	36.326434899266765	38.0	35.0	40.0	30.0	41.0
34	36.05759766844771	38.0	35.0	40.0	30.0	41.0
35	35.836330580806326	38.0	35.0	40.0	29.0	41.0
36	35.64832327157495	38.0	35.0	40.0	29.0	41.0
37	35.55123632410427	38.0	35.0	40.0	28.0	41.0
38	35.348035065806215	38.0	34.0	40.0	27.0	41.0
39	35.260612032475194	38.0	34.0	40.0	27.0	41.0
40	35.03168875627414	38.0	34.0	40.0	26.0	41.0
41	34.82167418407235	38.0	34.0	40.0	25.0	40.0
42	34.829545023477436	38.0	34.0	40.0	26.0	41.0
43	34.71374366803136	37.0	34.0	40.0	25.0	40.0
44	34.48800869706012	37.0	34.0	40.0	24.0	40.0
45	34.29166238752804	37.0	33.0	40.0	24.0	40.0
46	34.22096639141397	37.0	33.0	39.0	24.0	40.0
47	34.052300788749335	36.0	33.0	39.0	24.0	40.0
48	33.922731246964126	36.0	33.0	39.0	23.0	40.0
49	33.78089699997687	36.0	33.0	39.0	24.0	40.0
50	33.87720028681794	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	5.0
13	7.0
14	8.0
15	21.0
16	60.0
17	164.0
18	360.0
19	804.0
20	1462.0
21	2289.0
22	3874.0
23	5956.0
24	8979.0
25	13275.0
26	16748.0
27	18283.0
28	19315.0
29	21337.0
30	26119.0
31	34212.0
32	46734.0
33	77854.0
34	86416.0
35	113373.0
36	158940.0
37	277883.0
38	115119.0
39	31226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.36178382254296	22.29611639257049	26.944324936969444	15.3977748479171
2	34.973099252885525	23.28591585131728	29.348321883746216	12.39266301205098
3	31.32884602040108	23.43256308838156	32.58723660167002	12.651354289547337
4	27.577359887123265	26.75983623620845	30.43878518724123	15.224018689427057
5	17.293548909397913	47.85594337658733	23.158513172807808	11.691994541206949
6	20.213448060509336	41.10184349917887	28.811047116785787	9.87366132352601
7	38.928596211227536	55.51870099229755	4.401360072167095	1.1513427243078205
8	85.89623667106146	3.163324312446511	8.83917377928897	2.1012652372030627
9	81.07584484074664	4.933176046075914	10.741424374898804	3.2495547382786296
10	43.36594730876877	25.73839428214558	17.397173455462262	13.498484953623391
11	35.83392316054865	23.601785672981286	23.940785973677514	16.623505192792543
12	32.52191612888303	21.786598200448733	29.211667013623853	16.479818657044387
13	23.927000208174313	28.897555108366298	30.063146207757963	17.112298475701433
14	19.537020331691068	31.787384636735826	31.258251798394742	17.417343233178357
15	17.94675363726783	23.752596396271368	40.34038812943816	17.960261837022646
16	20.76941225452779	19.369925751162306	39.02139569310481	20.839266301205097
17	20.641824532186064	19.84188004533574	30.791108643860017	28.725186778618184
18	22.834038812943817	22.84773205653089	33.51754446834594	20.800684662179354
19	27.729836004903664	24.87960585663729	27.026299354659635	20.364258783799414
20	27.73899567460042	24.304952235560798	26.88335299424051	21.072699095598267
21	24.69261906414082	25.277727661739874	30.094511137325654	19.935142136793655
22	23.630004857400596	22.798602919066454	28.444382763167024	25.127009460365922
23	21.741817593042352	27.26204519695603	28.097980709180487	22.89815650082113
24	21.8281405407906	25.161797700830384	33.97386255869359	19.036199199685424
25	22.93053917146624	23.861032081974418	31.58318876783938	21.62523997871996
26	20.116485092406265	27.33680290518817	28.772557999676174	23.774154002729396
27	20.397104064025164	26.394559711331624	30.82062313510513	22.38771308953808
28	19.570698309161983	25.599241320287742	34.438137533828325	20.39192283672195
29	20.579279716882937	25.261443804501194	33.0234774362177	21.13579904239817
30	22.733560012027848	25.297619873707582	31.852057456109918	20.11676265815465
31	27.67043693474892	24.28237688802535	27.033331020285427	21.013855156940302
32	27.25177526426572	24.95353086762427	27.24751925612379	20.547174611986215
33	25.995050077487104	24.16746466819328	27.56163116138135	22.275854092938268
34	21.38088959822358	25.931117433442047	29.872643582448593	22.81534938588578
35	22.045567043693477	24.724631647121413	30.29019498993824	22.93960631924687
36	27.867046006522795	24.92272106955335	26.444243980292832	20.765988943631022
37	22.717368676705295	29.208613790391595	27.021488215020934	21.052529317882176
38	22.784539587814866	29.566488561978115	25.87930516040987	21.769666689797145
39	21.838688039229293	27.131496773298174	26.67369833229246	24.356116855180073
40	23.458376702981518	25.06825804362408	27.91053130710337	23.56283394629103
41	20.95935974834039	23.61455369740707	28.915966969675942	26.510119584576596
42	22.247819952351215	26.417227580783198	26.14465801586751	25.19029445099808
43	21.809266069900307	25.678255036661806	27.4781763930331	25.03430250040478
44	21.937223879906554	26.58820808178937	28.92290611338561	22.551661924918463
45	21.25246917863669	28.95750931001781	26.816644692711588	22.973376818633913
46	23.023986306756413	27.1898781023755	28.39775171743807	21.38838387343002
47	22.59283417759582	26.637892350750587	28.211875187935142	22.557398283718456
48	21.896329192977586	26.33405037818333	29.226563042120603	22.54305738671848
49	22.153170032151365	25.46452941040409	30.178335993338422	22.203964564106123
50	21.146716628501377	27.499363911826613	28.85980616658571	22.494113293086297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	539.0
1	1132.0
2	1725.0
3	8569.5
4	15414.0
5	11034.0
6	6654.0
7	6582.0
8	6510.0
9	6574.5
10	6639.0
11	6510.5
12	6382.0
13	6187.0
14	5992.0
15	5568.0
16	5144.0
17	5042.0
18	4940.0
19	4953.5
20	4967.0
21	5224.5
22	5482.0
23	6082.5
24	6683.0
25	7681.5
26	8680.0
27	10344.5
28	12009.0
29	13993.0
30	15977.0
31	18362.0
32	20747.0
33	23777.0
34	26807.0
35	28475.0
36	30143.0
37	33940.5
38	37738.0
39	45661.5
40	53585.0
41	62453.0
42	71321.0
43	74126.0
44	76931.0
45	80571.0
46	84211.0
47	85973.0
48	87735.0
49	84304.0
50	80873.0
51	74754.0
52	68635.0
53	64657.5
54	60680.0
55	58451.0
56	56222.0
57	53851.0
58	51480.0
59	47916.5
60	44353.0
61	40001.5
62	35650.0
63	31040.0
64	26430.0
65	22563.5
66	18697.0
67	15767.0
68	12837.0
69	11154.0
70	9471.0
71	7864.0
72	6257.0
73	4752.0
74	3247.0
75	2484.5
76	1722.0
77	1226.5
78	731.0
79	508.0
80	285.0
81	230.5
82	176.0
83	124.5
84	73.0
85	47.0
86	21.0
87	17.5
88	14.0
89	14.5
90	15.0
91	8.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1080825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.262329799440092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.84102170302991	20.53027372708064
2	8.196373560952756	4.96082719721927
3	4.521401731157284	4.104844510321232
4	3.0350418947961337	3.673897551017527
5	2.3197165998260805	3.5100014392586316
6	1.903561278842489	3.45637195282652
7	1.4866483607429462	3.1492610092020015
8	1.2597285541330543	3.049785677035705
9	1.063757654274183	2.8972606464291686
>10	8.210893945977308	44.772238758459
>50	0.12662348316999894	2.4571790945142498
>100	0.030696519926947108	1.7970925366260573
>500	0.0031394168106937885	0.5463904469895094
>1k	0.0013952963603083503	1.0945754530205223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4989	0.46159183956699745	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2217	0.20512108805773366	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2154	0.19929220734161407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009529757361274952	0.0
2	0.0	0.0	0.0	0.030254666574144752	0.0
3	0.0	0.0	0.0	0.04237503758702843	0.0
4	0.0	0.0	0.0	0.059306548238614025	0.0
5	0.0	0.0	0.0	0.0970554900191983	0.0
6	0.0	0.0	0.0	0.18199060902551292	0.0
7	0.0	0.0	0.0	0.2480512571415354	0.0
8	0.0	0.0	0.0	0.5967663590312955	0.0
9	0.0	0.0	0.0	0.7638609395600583	0.0
10	0.0	0.0	0.0	1.041704253695094	0.0
11	0.0	0.0	0.0	1.2262854763722157	0.0
12	0.0	0.0	0.0	1.400781810191289	0.0
13	0.0	0.0	0.0	1.4709134226169824	0.0
14	0.0	0.0	0.0	1.5018157426040293	0.0
15	0.0	0.0	0.0	1.543265561029769	0.0
16	0.0	0.0	0.0	1.629403464945759	0.0
17	0.0	0.0	0.0	1.72127772766174	0.0
18	0.0	0.0	0.0	1.8641315661647353	0.0
19	0.0	0.0	0.0	1.931580043022691	0.0
20	0.0	0.0	0.0	2.004209747183864	0.0
21	0.0	0.0	0.0	2.0941410496611383	0.0
22	0.0	0.0	0.0	2.1986908148867763	0.0
23	0.0	0.0	0.0	2.3117525963962713	0.0
24	0.0	0.0	0.0	2.3926167510929153	0.0
25	0.0	0.0	0.0	2.464968889505702	0.0
26	0.0	0.0	0.0	2.535100501931395	0.0
27	0.0	0.0	0.0	2.6131889991441724	0.0
28	0.0	0.0	0.0	2.6985867277311315	0.0
29	0.0	0.0	0.0	2.7924039506858187	0.0
30	0.0	0.0	0.0	2.9119422663243357	0.0
31	0.0	0.0	0.0	3.013716374066107	0.0
32	0.0	0.0	0.0	3.1094765572595007	0.0
33	0.0	0.0	0.0	3.2039414336270906	0.0
34	0.0	0.0	0.0	3.3108042467559504	0.0
35	0.0	0.0	0.0	3.4420003238267065	0.0
36	0.0	0.0	0.0	3.5431267781555755	0.0
37	0.0	0.0	0.0	3.6507297666134666	0.0
38	0.0	0.0	0.0	3.79043785996808	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	70	0.0	44.0	1
ACGCTAA	25	4.4426626E-5	44.0	13
ACGTATC	30	2.5275767E-6	44.0	21
TATACGC	20	7.856274E-4	44.0	22
CGTTTTA	2645	0.0	43.500946	1
CGGTCTA	200	0.0	42.9	31
CGTTATT	1530	0.0	41.555553	1
AGGCGAT	765	0.0	41.124184	7
CGTTAAG	65	0.0	40.615383	2
GTTTTAT	3090	0.0	40.084145	2
TCGCCGT	55	7.8216544E-11	40.000004	25
TCACGAC	225	0.0	39.11111	25
CGTTCGA	45	2.35068E-8	39.11111	14
TTACTGG	1595	0.0	39.03448	40
ACATACG	265	0.0	39.01887	17
CGTAAAG	125	0.0	38.72	2
TTACGGG	285	0.0	38.596493	3
CGTTTTT	1325	0.0	38.520756	1
ATATGCT	535	0.0	38.24299	34
GTTATTT	1890	0.0	38.179893	2
>>END_MODULE