FastQCFastQC Report
Thu 26 May 2016
SRR1545749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545749_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences396424
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.3208685649708393No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.3001836417573104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG351.2092278E-745.0000045
CGCTATG351.2092278E-745.00000424
AGGTACC1050.045.07
TACTCGC253.886166E-545.045
TCGTAAG253.886166E-545.02
ATGGTCG253.886166E-545.044
CGAGAAT207.02769E-445.045
ATAACGG207.02769E-445.02
ATAACGC406.7957444E-945.011
TCGACTA207.02769E-445.01
AGGCGAT2600.044.1346177
CGTTTTA6850.043.3576661
CGTTTTT9100.042.7747231
GGTACCT1750.042.428578
CGGTAGT750.042.00000412
CGGTCTA650.041.53846431
TAACGGG603.6379788E-1241.2500043
ATCCTGC1750.041.14285720
GCAAGGA5100.041.029414
ATAGGCG556.002665E-1140.9090925