##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545749_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	396424
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97653017980748	31.0	31.0	33.0	30.0	33.0
2	31.14152271305471	31.0	31.0	33.0	30.0	34.0
3	31.079092083224023	31.0	31.0	34.0	28.0	34.0
4	35.063951728452366	35.0	35.0	37.0	32.0	37.0
5	32.701241599903135	35.0	35.0	37.0	28.0	37.0
6	33.92125855144996	35.0	35.0	37.0	28.0	37.0
7	15.933964643916614	0.0	0.0	35.0	0.0	37.0
8	25.213579399834522	17.0	17.0	35.0	17.0	37.0
9	32.74467237099671	32.0	32.0	37.0	27.0	39.0
10	35.446590014731704	37.0	34.0	37.0	32.0	39.0
11	36.39884568038262	37.0	35.0	39.0	32.0	39.0
12	36.39886586079551	37.0	35.0	39.0	32.0	39.0
13	36.25012612758057	38.0	35.0	39.0	32.0	39.0
14	37.2596638952233	39.0	36.0	40.0	32.0	41.0
15	37.513556192359694	39.0	36.0	41.0	32.0	41.0
16	37.50270922043065	39.0	36.0	41.0	32.0	41.0
17	37.447868948398686	39.0	36.0	41.0	32.0	41.0
18	37.39565969769742	39.0	36.0	40.0	32.0	41.0
19	37.34729229310032	39.0	36.0	40.0	32.0	41.0
20	37.45178394849959	39.0	36.0	41.0	32.0	41.0
21	37.341212943716826	39.0	35.0	41.0	32.0	41.0
22	37.391260367687124	39.0	35.0	41.0	32.0	41.0
23	37.358641252800034	39.0	35.0	41.0	32.0	41.0
24	37.18641404153129	39.0	35.0	41.0	32.0	41.0
25	36.92481030411882	39.0	35.0	40.0	31.0	41.0
26	36.819733416745706	39.0	35.0	40.0	31.0	41.0
27	36.76135904990616	39.0	35.0	40.0	31.0	41.0
28	36.64978406958206	38.0	35.0	40.0	31.0	41.0
29	36.583857183217965	38.0	35.0	40.0	31.0	41.0
30	36.2986776784453	38.0	35.0	40.0	30.0	41.0
31	36.170796924505076	38.0	35.0	40.0	30.0	41.0
32	36.08129427078078	38.0	35.0	40.0	30.0	41.0
33	35.90211742982262	38.0	35.0	40.0	29.0	41.0
34	35.69271033035336	38.0	35.0	40.0	27.0	41.0
35	35.47945886222832	38.0	34.0	40.0	27.0	41.0
36	35.288390712973985	38.0	34.0	40.0	25.0	41.0
37	35.1565369402458	38.0	34.0	40.0	25.0	41.0
38	35.00175821847315	38.0	34.0	40.0	25.0	41.0
39	34.87579208120598	38.0	34.0	40.0	24.0	41.0
40	34.73937501261276	38.0	34.0	40.0	24.0	41.0
41	34.59275926785462	38.0	33.0	40.0	23.0	41.0
42	34.56786672855327	38.0	33.0	40.0	23.0	41.0
43	34.481711500817305	38.0	33.0	40.0	23.0	41.0
44	34.26518828325228	37.0	33.0	40.0	23.0	41.0
45	34.16253556797772	37.0	33.0	40.0	23.0	41.0
46	34.13097592476742	37.0	33.0	40.0	23.0	41.0
47	34.07124694771255	37.0	33.0	40.0	23.0	41.0
48	33.97601557927875	37.0	33.0	40.0	23.0	41.0
49	33.87263636914011	36.0	33.0	40.0	23.0	41.0
50	33.753776259762276	36.0	33.0	40.0	22.0	41.0
51	33.58467196738845	36.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	10.0
15	29.0
16	47.0
17	110.0
18	230.0
19	462.0
20	842.0
21	1371.0
22	2154.0
23	3048.0
24	4284.0
25	5857.0
26	6869.0
27	7531.0
28	8061.0
29	9333.0
30	11264.0
31	14571.0
32	18848.0
33	27043.0
34	34740.0
35	39767.0
36	50422.0
37	74454.0
38	61503.0
39	13568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.999142332452124	23.118176497891145	27.03847395717716	14.844207212479569
2	34.74310334389442	23.080590478881195	29.751225960083143	12.425080217141243
3	31.983431881016283	23.169384295602686	32.08761326256735	12.759570560813675
4	28.012683389502147	27.255917906080356	29.2426795552237	15.488719149193791
5	21.26006498092951	36.44910499868828	27.943313220188486	14.347516800193732
6	20.18772829092083	41.74469759651282	28.373408269933204	9.69416584263314
7	39.222145985106856	54.96362480576352	4.884164430004238	0.930064779125381
8	86.36207696809477	3.4455532460194136	8.170796924505074	2.0215728613807435
9	81.27787419530603	5.156095493713801	10.092728997235284	3.4733013137448796
10	43.36846406877485	26.414394688515326	16.84484289548564	13.372298347224184
11	35.97511755090509	24.067412669263213	23.40625189191371	16.55121788791799
12	32.735404516376406	22.250166488406354	28.711682441022745	16.3027465541945
13	24.166044437269186	29.605926987266155	29.28581518777874	16.94221338768591
14	19.529846830666155	32.61634008031805	30.65858777470587	17.19522531430993
15	17.807953100720443	24.312352430730733	39.91736120921034	17.962333259338486
16	20.675085262244465	19.82725566565092	38.76430286763667	20.733356204467942
17	20.639265029362498	20.126682541924808	30.30013319072508	28.93391923798761
18	22.889633321897765	23.308124634230015	33.18593223417351	20.616309809698706
19	28.037656650455066	25.171281254414463	26.512269691037876	20.278792404092588
20	28.20843339454725	24.460930720642544	26.263546102153253	21.067089782656954
21	24.697041551470143	25.925776441385988	29.5055294331322	19.871652574011662
22	23.574506084394486	23.11893326337457	27.944069985671906	25.36249066655904
23	21.673763445200088	27.777329324157975	27.73444594676407	22.81446128387787
24	21.664430004237886	25.62584505478982	33.78806530381612	18.92165963715618
25	23.011220309567534	24.381974855205538	31.283171553689986	21.323633281536942
26	20.04797893164894	27.884033257320446	28.440760397957742	23.62722741307287
27	20.332270498254395	27.242043872217625	30.356638346820574	22.069047282707405
28	19.5394325267895	26.052156277117426	34.15282626682542	20.255584929267652
29	20.386253102738483	25.745666256331607	32.93418158335519	20.933899057574717
30	22.65099993945876	26.027183016164514	31.561156741266927	19.760660303109802
31	27.994016507577747	24.634482271507274	26.551873751336952	20.819627469578027
32	27.587381187819105	25.32490464754909	26.87324682663007	20.214467338001736
33	26.414646943676466	24.45411983129175	27.068240066191755	22.06299315884003
34	21.283020200593302	26.330645975016647	29.449528787358993	22.936805037031057
35	22.133120093637114	24.95812564325066	30.15861804532521	22.750136217787016
36	28.143099307811838	25.42832926361673	25.994894355538516	20.433677073032914
37	22.504187435674933	29.83043408068129	26.743335418642666	20.92204306500111
38	22.67975702782879	30.133644784372287	25.440942021673763	21.74565616612516
39	21.79661170867556	27.823744273807844	26.277924646338263	24.101719371178334
40	23.553568906019816	25.295895304017918	27.69534639678728	23.455189393174987
41	20.98031400722459	23.93472645450326	28.62944725849091	26.455512279781246
42	22.179535043286986	26.64823522289266	25.853379210138637	25.318850523681714
43	21.700502492280993	25.765846669222853	27.272062236393356	25.261588602102798
44	21.717655843238553	26.846003269226888	29.11150687143059	22.32483401610397
45	21.225001513530966	29.579187940185257	26.52135087683894	22.67445966944484
46	22.926210320263152	27.934232034387424	28.074990414303873	21.064567231045547
47	22.51074606986459	27.123736201642686	27.621435634573082	22.74408209391964
48	21.681835610356586	26.891661453393333	29.224012673299292	22.202490262950782
49	22.0423082356265	26.121526446431094	30.005246907351722	21.83091841059068
50	20.984350089802835	28.360038746392753	28.599933404637458	22.055677759166954
51	21.092063043609873	29.182895082033376	27.83585252154259	21.88918935281416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	410.5
2	595.0
3	2664.0
4	4733.0
5	3437.0
6	2141.0
7	2133.0
8	2125.0
9	2148.5
10	2172.0
11	2107.0
12	2042.0
13	2030.0
14	2018.0
15	1889.0
16	1760.0
17	1729.0
18	1698.0
19	1771.0
20	1844.0
21	1886.0
22	1928.0
23	2115.5
24	2303.0
25	2655.5
26	3476.5
27	3945.0
28	4633.0
29	5321.0
30	6282.5
31	7244.0
32	8505.5
33	9767.0
34	10448.0
35	11129.0
36	12184.0
37	13239.0
38	15592.5
39	17946.0
40	21024.0
41	24102.0
42	25852.0
43	27602.0
44	28387.0
45	29172.0
46	29821.0
47	30470.0
48	29978.0
49	29486.0
50	28138.0
51	26790.0
52	25175.0
53	23560.0
54	22539.5
55	21519.0
56	20456.5
57	19394.0
58	18402.5
59	17411.0
60	15783.0
61	14155.0
62	12504.5
63	10854.0
64	9452.0
65	8050.0
66	6790.0
67	5530.0
68	4847.5
69	4165.0
70	3554.0
71	2943.0
72	2439.5
73	1936.0
74	1513.0
75	797.5
76	505.0
77	378.5
78	252.0
79	188.5
80	125.0
81	98.5
82	72.0
83	53.5
84	35.0
85	24.5
86	14.0
87	9.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	396424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.042894593136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.45266978779092	28.603152611083576
2	11.072479770882108	9.531831593254184
3	6.039586957534034	7.798839143976491
4	4.098720930110595	7.0568325184572265
5	2.930250748266958	6.306323704455628
6	2.2444733027411163	5.796517667219621
7	1.614675053563022	4.865020169287557
8	1.2631422407229884	4.349543865886176
9	0.9686078020008315	3.752251517124984
>10	3.2719883023486434	19.282435740150756
>50	0.025221884779283575	0.7314068889305329
>100	0.017010108339516734	1.304262336284127
>500	0.0	0.0
>1k	0.0011731109199666714	0.6215822438891833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1272	0.3208685649708393	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1190	0.3001836417573104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010090206445623877	0.0
2	0.0	0.0	0.0	0.032540915787137005	0.0
3	0.0	0.0	0.0	0.04389239803846387	0.0
4	0.0	0.0	0.0	0.06003672835146207	0.0
5	0.0	0.0	0.0	0.10392912638992594	0.0
6	0.0	0.0	0.0	0.17153350957560592	0.0
7	0.0	0.0	0.0	0.21492139729178858	0.0
8	0.0	0.0	0.0	0.4833208887453837	0.0
9	0.0	0.0	0.0	0.6243315238229774	0.0
10	0.0	0.0	0.0	0.8450547898209997	0.0
11	0.0	0.0	0.0	0.9878312110265776	0.0
12	0.0	0.0	0.0	1.124553508364781	0.0
13	0.0	0.0	0.0	1.1780316025265878	0.0
14	0.0	0.0	0.0	1.2040138841240693	0.0
15	0.0	0.0	0.0	1.2294916553992694	0.0
16	0.0	0.0	0.0	1.2907896595564345	0.0
17	0.0	0.0	0.0	1.3737816075716909	0.0
18	0.0	0.0	0.0	1.4978911468528646	0.0
19	0.0	0.0	0.0	1.5523782616592336	0.0
20	0.0	0.0	0.0	1.6083789074324462	0.0
21	0.0	0.0	0.0	1.681028393840938	0.0
22	0.0	0.0	0.0	1.762506810889351	0.0
23	0.0	0.0	0.0	1.8596250479284806	0.0
24	0.0	0.0	0.0	1.9418602304603152	0.0
25	0.0	0.0	0.0	1.9981131313946683	0.0
26	0.0	0.0	0.0	2.064203983613505	0.0
27	0.0	0.0	0.0	2.1376102355054183	0.0
28	0.0	0.0	0.0	2.211268742558473	0.0
29	0.0	0.0	0.0	2.2846749944503864	0.0
30	0.0	0.0	0.0	2.396424030835671	0.0
31	0.0	0.0	0.0	2.477650192722943	0.0
32	0.0	0.0	0.0	2.571993622989526	0.0
33	0.0	0.0	0.0	2.6615442051944385	0.0
34	0.0	0.0	0.0	2.758662442233568	0.0
35	0.0	0.0	0.0	2.8772223679696487	0.0
36	0.0	0.0	0.0	2.9791334530704496	0.0
37	0.0	0.0	0.0	3.0742336488204547	0.0
38	0.0	0.0	0.0	3.197586422618207	0.0
39	0.0	0.0	0.0	3.4016608479809496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	35	1.2092278E-7	45.000004	5
CGCTATG	35	1.2092278E-7	45.000004	24
AGGTACC	105	0.0	45.0	7
TACTCGC	25	3.886166E-5	45.0	45
TCGTAAG	25	3.886166E-5	45.0	2
ATGGTCG	25	3.886166E-5	45.0	44
CGAGAAT	20	7.02769E-4	45.0	45
ATAACGG	20	7.02769E-4	45.0	2
ATAACGC	40	6.7957444E-9	45.0	11
TCGACTA	20	7.02769E-4	45.0	1
AGGCGAT	260	0.0	44.134617	7
CGTTTTA	685	0.0	43.357666	1
CGTTTTT	910	0.0	42.774723	1
GGTACCT	175	0.0	42.42857	8
CGGTAGT	75	0.0	42.000004	12
CGGTCTA	65	0.0	41.538464	31
TAACGGG	60	3.6379788E-12	41.250004	3
ATCCTGC	175	0.0	41.142857	20
GCAAGGA	510	0.0	41.02941	4
ATAGGCG	55	6.002665E-11	40.909092	5
>>END_MODULE