FastQCFastQC Report
Thu 26 May 2016
SRR1545748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545748_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1501844
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101060.6729061074252718No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.3002309161271077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT29750.043.3344541
CGTTTTA60150.043.1221921
GTTTTAT66850.040.7419622
AGGCGAT13350.040.7041177
TACGGGA3300.040.04
GTTATTT34850.039.7704472
CGTTTTT28950.039.59241
GTACGGG4950.038.6666683
CTACGGG2100.037.714293
ACGGGAC2000.037.3999985
TTACTGG22400.037.22321340
GCAAGGA18450.036.964774
TTACGGG3100.036.9032253
GCTAACG301.3012855E-436.6666641
TCGAATA301.3012855E-436.6666641
AAGGCGA7900.036.481016
CGAAGGA3200.036.43754
ACAACGA1150.036.34782813
GCGCGAC9450.036.3174559
TACGGGG7150.036.3076934
Produced by FastQC (version 0.11.5)