##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545748_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1501844
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.464946425860475	31.0	31.0	33.0	30.0	34.0
2	31.65532571958206	31.0	31.0	34.0	30.0	34.0
3	31.66683490429099	31.0	31.0	34.0	30.0	34.0
4	35.60565811096226	37.0	35.0	37.0	33.0	37.0
5	27.090789056653023	35.0	25.0	37.0	0.0	37.0
6	31.319900735362662	35.0	26.0	37.0	17.0	37.0
7	15.028529594285425	0.0	0.0	32.0	0.0	37.0
8	25.100424544759644	17.0	17.0	35.0	17.0	37.0
9	33.14442112496371	32.0	32.0	37.0	28.0	39.0
10	35.92372443476153	37.0	35.0	39.0	32.0	39.0
11	36.93034562844077	38.0	37.0	39.0	34.0	39.0
12	37.14137620152293	39.0	37.0	39.0	34.0	39.0
13	36.84721515683387	39.0	37.0	39.0	33.0	39.0
14	38.17356796045395	40.0	37.0	41.0	33.0	41.0
15	38.38462583330892	40.0	38.0	41.0	34.0	41.0
16	38.38091373005452	40.0	37.0	41.0	34.0	41.0
17	38.37292421849406	40.0	37.0	41.0	34.0	41.0
18	38.33820822935005	40.0	37.0	41.0	34.0	41.0
19	38.28178026479448	40.0	37.0	41.0	34.0	41.0
20	38.31280013103891	40.0	37.0	41.0	34.0	41.0
21	38.186623244491436	40.0	37.0	41.0	34.0	41.0
22	38.05635072617396	40.0	36.0	41.0	34.0	41.0
23	37.92341814462754	40.0	36.0	41.0	34.0	41.0
24	37.74622397532633	40.0	35.0	41.0	33.0	41.0
25	37.55641398174511	39.0	35.0	41.0	33.0	41.0
26	37.29778791938444	39.0	35.0	40.0	33.0	41.0
27	37.1035526992151	39.0	35.0	40.0	32.0	41.0
28	37.10272505000519	39.0	35.0	40.0	32.0	41.0
29	36.97968963487553	39.0	35.0	40.0	32.0	41.0
30	36.73757660582591	39.0	35.0	40.0	31.0	41.0
31	36.530452563648424	38.0	35.0	40.0	31.0	41.0
32	36.29957505573149	38.0	35.0	40.0	30.0	41.0
33	36.01314850277392	38.0	35.0	40.0	30.0	41.0
34	35.67736862150796	38.0	35.0	40.0	28.0	41.0
35	35.41693677905295	38.0	35.0	40.0	26.0	41.0
36	35.20772330548313	38.0	35.0	40.0	25.0	41.0
37	35.11262221642195	38.0	34.0	40.0	25.0	41.0
38	34.912624080796675	38.0	34.0	40.0	24.0	41.0
39	34.79721395830725	38.0	34.0	40.0	24.0	41.0
40	34.557216328726554	38.0	34.0	40.0	23.0	41.0
41	34.31053092065488	38.0	34.0	40.0	23.0	40.0
42	34.30512556563797	38.0	33.0	40.0	23.0	40.0
43	34.15940603684537	37.0	33.0	40.0	22.0	40.0
44	33.93331730858864	37.0	33.0	40.0	22.0	40.0
45	33.766769384836245	37.0	33.0	40.0	22.0	40.0
46	33.67402273471812	36.0	33.0	39.0	22.0	40.0
47	33.49937077352907	36.0	33.0	39.0	21.0	40.0
48	33.37840681189258	36.0	33.0	39.0	21.0	40.0
49	33.22228007702531	36.0	32.0	39.0	20.0	40.0
50	33.34394384503317	36.0	33.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	6.0
13	12.0
14	18.0
15	39.0
16	97.0
17	285.0
18	651.0
19	1299.0
20	2527.0
21	4222.0
22	6678.0
23	10243.0
24	15791.0
25	23051.0
26	29015.0
27	30434.0
28	30052.0
29	31735.0
30	37170.0
31	47413.0
32	63182.0
33	98686.0
34	115977.0
35	153897.0
36	222550.0
37	379972.0
38	153897.0
39	42943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.584454843512376	21.727689427130915	25.343511043756873	17.344344685599836
2	38.33613877340123	22.17826884816266	27.454316160666487	12.031276217769623
3	31.12194076082469	22.42676336556926	34.08090320965427	12.370392663951781
4	26.627599138126197	25.96960802853026	32.20427687562756	15.19851595771598
5	16.98964739347096	47.07053462277041	24.54429354846442	11.395524435294211
6	20.09895834720517	39.62648584007394	30.780360676608222	9.494195136112673
7	37.85759373143948	55.5115577916215	5.5383914707519555	1.0924570061870607
8	83.72440812760846	3.0438580837956537	11.245775193695218	1.9859585949006688
9	79.03630470275208	4.535424451540906	13.014600717517933	3.4136701281890796
10	39.23703127621777	27.027374347801768	19.784012187683942	13.951582188296522
11	32.56916164395236	24.58577588617726	26.53091799148247	16.314144478387902
12	29.40684918007463	22.159758270499466	31.86762406747971	16.565768481946193
13	23.941101738928943	26.179350185505285	32.49079132053662	17.38875675502915
14	20.26042651567007	28.881495015460995	33.56839991370608	17.28967855516285
15	19.559621372126532	23.67416322867089	38.77879460183614	17.98742079736644
16	22.24884874860505	20.16880581471844	37.87131020265753	19.71103523401898
17	22.241191495255165	20.369958530979247	32.57069309462234	24.818156879143242
18	23.332782898889633	22.973491254750826	33.65695771331776	20.03676813304178
19	26.89307278252602	24.496818577695155	29.24391614575149	19.36619249402734
20	27.68836177392592	24.121213654680513	28.666292903923445	19.524131667470122
21	25.612313928743596	24.08552419558889	31.331816087423192	18.97034578824432
22	23.824578318387264	22.122271021490914	30.71277709269405	23.340373567427775
23	22.746503631535635	25.783436894910523	29.920284663387143	21.549774810166703
24	22.16095679711075	24.68298971131489	33.57665643036161	19.57939706121275
25	23.26886147962105	23.595193641949496	32.04980011239516	21.08614476603429
26	21.477397119807385	26.04791176713427	30.13382215463124	22.34086895842711
27	20.794103781750966	24.85397950785834	32.50144489041472	21.850471819975976
28	20.71686540013477	24.893064792348607	34.29470704014532	20.09536276737131
29	21.40974695108147	24.90025595201632	33.26617145322683	20.423825643675375
30	22.96809788500004	24.190595028511616	32.79175466959285	20.049552416895498
31	26.444157981787725	23.843155480862194	29.31516189431126	20.397524643038825
32	26.393154015996334	24.269697784856483	29.232130634073844	20.105017565073336
33	25.629958903854195	24.190461858888142	28.863850040350396	21.315729196907267
34	21.932903816907746	25.632023033018076	30.700259148087284	21.73481400198689
35	22.706486159681035	25.26846996092803	30.323255944026144	21.70178793536479
36	26.230354151296677	25.663317894534988	27.188709346643193	20.91761860752515
37	22.567789996830562	28.42046177898637	27.549465856640236	21.462282367542834
38	23.05938566189298	28.546506827606592	26.518866140557872	21.87524136994255
39	22.65634779644224	26.829018193633956	26.85771624749308	23.65691776243072
40	23.978189479067066	24.88587363268089	28.328175229917356	22.807761658334687
41	22.08158770151893	24.51033529447799	28.798530340035317	24.609546663967762
42	23.071237758382363	26.8178985300737	26.490967104439612	23.619896607104334
43	22.64576081137588	25.3755383382029	27.919277901033663	24.059422949387553
44	22.661408242134335	26.12335235883354	29.33333954791576	21.88189985111636
45	22.229672322824477	27.56484694815174	27.805684212208458	22.39979651681533
46	23.281845517909982	26.614415345402055	28.96306140984017	21.140677726847795
47	22.429759682097476	26.87256466051068	28.82290038113146	21.874775276260383
48	22.435419391095213	26.307059854418966	29.384743022577574	21.87277773190824
49	22.53602904163149	25.395913290594763	29.993061862616887	22.07499580515686
50	21.500368879857028	26.801651836009597	29.641760395886656	22.056218888246715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	676.0
1	1540.5
2	2405.0
3	17002.5
4	31600.0
5	22286.0
6	12972.0
7	12847.5
8	12723.0
9	12522.0
10	12321.0
11	12150.5
12	11980.0
13	11452.0
14	10924.0
15	10504.0
16	10084.0
17	9559.0
18	9034.0
19	8751.0
20	8468.0
21	8687.0
22	8906.0
23	9570.0
24	10234.0
25	11497.5
26	12761.0
27	14723.5
28	16686.0
29	19059.5
30	21433.0
31	24571.0
32	27709.0
33	31412.0
34	35115.0
35	39149.5
36	43184.0
37	47975.5
38	52767.0
39	61106.0
40	69445.0
41	77902.5
42	86360.0
43	90098.5
44	93837.0
45	98222.0
46	102607.0
47	107648.0
48	112689.0
49	111451.0
50	110213.0
51	103209.0
52	96205.0
53	90360.5
54	84516.0
55	82401.5
56	80287.0
57	77359.0
58	74431.0
59	69553.5
60	64676.0
61	58591.5
62	52507.0
63	45966.5
64	39426.0
65	33456.5
66	27487.0
67	23810.5
68	20134.0
69	17638.0
70	15142.0
71	12378.5
72	9615.0
73	7481.0
74	5347.0
75	3995.0
76	2643.0
77	1981.5
78	1320.0
79	965.0
80	610.0
81	411.5
82	213.0
83	146.0
84	79.0
85	61.0
86	43.0
87	31.5
88	20.0
89	13.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1501844.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.72722955980822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.34861629870959	23.05209472001828
2	8.75796518151359	5.907638043074328
3	4.7863947418726145	4.842955026689901
4	3.336887191219568	4.501758412537842
5	2.3598003823935945	3.9794764606156
6	1.8578078093394648	3.7595226278157936
7	1.5062976060746178	3.5562271601827753
8	1.2480631266971252	3.367496926339677
9	1.0104165210574343	3.0670694961054195
>10	6.695078532620485	39.21705145050852
>50	0.06542556451787016	1.421968627653777
>100	0.0241669440305862	1.5756692033765947
>500	0.00260623842214034	0.5831845172000645
>1k	2.3693076564912182E-4	0.36031501617636214
>5k	0.0	0.0
>10k+	2.3693076564912182E-4	0.8075723117051045
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10106	0.6729061074252718	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4509	0.3002309161271077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0043945975747148175	0.0
2	0.0	0.0	0.0	0.014781828205858931	0.0
3	0.0	0.0	0.0	0.024370041096145805	0.0
4	0.0	0.0	0.0	0.035423119844670954	0.0
5	0.0	0.0	0.0	0.060192669811245374	0.0
6	0.0	0.0	0.0	0.15913770005406686	0.0
7	0.0	0.0	0.0	0.2260554358508607	0.0
8	0.0	0.0	0.0	0.5590460793531152	0.0
9	0.0	0.0	0.0	0.7604651348608777	0.0
10	0.0	0.0	0.0	1.028735341353696	0.0
11	0.0	0.0	0.0	1.2024551151784073	0.0
12	0.0	0.0	0.0	1.3418837109579957	0.0
13	0.0	0.0	0.0	1.4145277405642662	0.0
14	0.0	0.0	0.0	1.454278873171914	0.0
15	0.0	0.0	0.0	1.4913000284982993	0.0
16	0.0	0.0	0.0	1.5526246401090926	0.0
17	0.0	0.0	0.0	1.6230713709280058	0.0
18	0.0	0.0	0.0	1.7313382748141617	0.0
19	0.0	0.0	0.0	1.7832078431581442	0.0
20	0.0	0.0	0.0	1.8441995307102468	0.0
21	0.0	0.0	0.0	1.9092528917783738	0.0
22	0.0	0.0	0.0	1.9852927467832877	0.0
23	0.0	0.0	0.0	2.0709208146784888	0.0
24	0.0	0.0	0.0	2.1372392871696393	0.0
25	0.0	0.0	0.0	2.1927710201592174	0.0
26	0.0	0.0	0.0	2.2474371505962005	0.0
27	0.0	0.0	0.0	2.3107593065591367	0.0
28	0.0	0.0	0.0	2.3756794980037874	0.0
29	0.0	0.0	0.0	2.4511866745147963	0.0
30	0.0	0.0	0.0	2.5531280212858327	0.0
31	0.0	0.0	0.0	2.631365175078104	0.0
32	0.0	0.0	0.0	2.717792260714162	0.0
33	0.0	0.0	0.0	2.8075485869371253	0.0
34	0.0	0.0	0.0	2.9002346448765652	0.0
35	0.0	0.0	0.0	3.0083017943275068	0.0
36	0.0	0.0	0.0	3.0950618040222553	0.0
37	0.0	0.0	0.0	3.1894124822551477	0.0
38	0.0	0.0	0.0	3.3125943839706387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	2975	0.0	43.334454	1
CGTTTTA	6015	0.0	43.122192	1
GTTTTAT	6685	0.0	40.741962	2
AGGCGAT	1335	0.0	40.704117	7
TACGGGA	330	0.0	40.0	4
GTTATTT	3485	0.0	39.770447	2
CGTTTTT	2895	0.0	39.5924	1
GTACGGG	495	0.0	38.666668	3
CTACGGG	210	0.0	37.71429	3
ACGGGAC	200	0.0	37.399998	5
TTACTGG	2240	0.0	37.223213	40
GCAAGGA	1845	0.0	36.96477	4
TTACGGG	310	0.0	36.903225	3
GCTAACG	30	1.3012855E-4	36.666664	1
TCGAATA	30	1.3012855E-4	36.666664	1
AAGGCGA	790	0.0	36.48101	6
CGAAGGA	320	0.0	36.4375	4
ACAACGA	115	0.0	36.347828	13
GCGCGAC	945	0.0	36.317455	9
TACGGGG	715	0.0	36.307693	4
>>END_MODULE