FastQCFastQC Report
Thu 26 May 2016
SRR1545747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545747_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences566673
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.4584654642095177No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24160.42634817610861997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.163546E-645.0000042
CGTTAAG207.030255E-445.0000042
CTTGCGG207.030255E-445.0000042
CATACGA700.045.018
CGTTTTA13650.044.670331
CGTTATT3000.042.751
CGTTTTT19100.042.408381
TTCGAAT750.042.016
AGGCGAT4500.041.57
GTTTTAT16050.040.6542052
CGTTCGA800.039.37500414
GCAAGGA7350.038.877554
GACCGAT2150.038.7209329
GACACGA700.038.57142625
CGTAAAT356.2426843E-638.57142616
CGTTTAG356.2426843E-638.5714262
AGGTACC1700.038.3823557
CGTTGAT659.094947E-1238.0769225
GTAAGGC950.037.8947374
TTGCGGA301.1391914E-437.5000041
Produced by FastQC (version 0.11.5)