##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545747_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	566673
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939727144226037	31.0	31.0	33.0	30.0	33.0
2	31.116017526862937	31.0	31.0	33.0	30.0	34.0
3	31.0120369242932	31.0	31.0	34.0	28.0	34.0
4	34.99279478641121	35.0	35.0	37.0	32.0	37.0
5	32.739177620956	35.0	35.0	37.0	28.0	37.0
6	33.94783940650075	35.0	35.0	37.0	28.0	37.0
7	15.491593917479745	0.0	0.0	35.0	0.0	37.0
8	24.985947804112776	17.0	17.0	35.0	17.0	37.0
9	32.64951744656972	32.0	32.0	37.0	27.0	39.0
10	35.36781706557397	37.0	34.0	37.0	32.0	39.0
11	36.361908896312336	37.0	35.0	39.0	32.0	39.0
12	36.43544160388796	37.0	35.0	39.0	32.0	39.0
13	36.3580777626603	38.0	35.0	39.0	32.0	39.0
14	37.41109069957454	39.0	36.0	40.0	32.0	41.0
15	37.62439360971848	39.0	36.0	41.0	32.0	41.0
16	37.56042903049907	39.0	36.0	41.0	32.0	41.0
17	37.477730543011575	39.0	36.0	41.0	32.0	41.0
18	37.403989602469146	39.0	36.0	40.0	32.0	41.0
19	37.35200371289968	39.0	36.0	40.0	32.0	41.0
20	37.450946136484355	39.0	36.0	40.0	32.0	41.0
21	37.33507507857265	39.0	35.0	40.0	32.0	41.0
22	37.3471402378444	39.0	35.0	40.0	32.0	41.0
23	37.29878077833248	39.0	35.0	40.0	32.0	41.0
24	37.09676303617783	39.0	35.0	40.0	32.0	41.0
25	36.82874426697584	39.0	35.0	40.0	31.0	41.0
26	36.712252039536025	38.0	35.0	40.0	31.0	41.0
27	36.63873168476352	38.0	35.0	40.0	31.0	41.0
28	36.51386955086973	38.0	35.0	40.0	31.0	41.0
29	36.43984802522795	38.0	35.0	40.0	30.0	41.0
30	36.114540484547526	38.0	35.0	40.0	30.0	41.0
31	35.96688566421905	38.0	35.0	40.0	29.0	41.0
32	35.84486643972803	38.0	35.0	40.0	29.0	41.0
33	35.59126515644825	38.0	34.0	40.0	27.0	41.0
34	35.33481919907954	38.0	34.0	40.0	25.0	41.0
35	35.095485403398435	38.0	34.0	40.0	24.0	41.0
36	34.9081851438131	38.0	34.0	40.0	24.0	41.0
37	34.79099057128185	38.0	34.0	40.0	23.0	41.0
38	34.614511367225894	38.0	33.0	40.0	23.0	41.0
39	34.47471998842366	38.0	33.0	40.0	23.0	41.0
40	34.335419192373735	38.0	33.0	40.0	22.0	41.0
41	34.13242028471446	38.0	33.0	40.0	21.0	41.0
42	34.07673031889644	38.0	33.0	40.0	21.0	41.0
43	33.9870330861008	37.0	33.0	40.0	20.0	41.0
44	33.76126619761308	37.0	33.0	40.0	19.0	41.0
45	33.69517340688545	37.0	33.0	40.0	20.0	41.0
46	33.63857109832302	37.0	33.0	40.0	20.0	41.0
47	33.54582978190244	37.0	32.0	40.0	20.0	41.0
48	33.45947486469269	36.0	32.0	40.0	20.0	41.0
49	33.32193169605751	36.0	32.0	40.0	19.0	41.0
50	33.22394749705739	36.0	32.0	40.0	19.0	40.0
51	33.07368447058533	36.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	5.0
14	15.0
15	27.0
16	71.0
17	197.0
18	477.0
19	923.0
20	1449.0
21	2365.0
22	3580.0
23	5374.0
24	7476.0
25	9889.0
26	11621.0
27	12253.0
28	12817.0
29	13838.0
30	16581.0
31	20612.0
32	26733.0
33	37107.0
34	47443.0
35	55795.0
36	71652.0
37	104658.0
38	84469.0
39	19239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.47266236436181	22.433043395397345	25.59253749516917	16.50175674507167
2	37.925576125913885	22.015342181469734	27.983863709758538	12.075217982857838
3	31.891584741111718	22.214751717480805	33.32715693177547	12.566506609632011
4	27.149343624983015	26.70252508942547	30.53065877499016	15.617472510601353
5	20.756591543976864	35.54519096551274	29.470082393196783	14.228135097313618
6	20.207950617022515	40.364372398190845	30.106428222272807	9.32124876251383
7	37.14276134560849	56.11066699842766	5.874110818761437	0.8724608372024079
8	84.50129086792559	3.3400214938774218	10.233415038302512	1.9252725998944717
9	79.74404991944208	4.7281236268535825	11.939690085816688	3.588136367887653
10	39.312619447194415	27.734160618204857	18.886906558103174	14.066313376497558
11	32.81945672371897	25.09966065085155	25.6786541797474	16.402228445682077
12	29.885665983733123	22.554277334547436	31.081417325335774	16.478639356383663
13	24.21802344562031	26.89522881803086	31.609764361457138	17.276983374891692
14	20.43577160019976	29.817196160748793	32.58051821773756	17.16651402131388
15	19.53419344136742	24.29655197971317	38.181808556257316	17.9874460226621
16	22.29645668666056	20.618946023544442	37.348170814561485	19.73642647523351
17	22.112223451620245	20.77070903325198	31.861761545018023	25.25530597010975
18	23.483208128850325	23.349268449352625	33.12421802344562	20.04330539835143
19	27.258577698249255	24.818546145660726	28.475858210996464	19.447017945093556
20	28.050745315199414	24.586666384316878	27.792395261464726	19.570193039018978
21	25.788947064709276	24.729958900459348	30.467835947715876	19.0132580871155
22	23.752322768157295	22.52445413845375	30.08719314313546	23.636029950253498
23	22.83256834188324	26.513350733138864	29.054851739892317	21.599229185085576
24	22.1626934757788	25.259011811044463	33.04286599149774	19.535428721678993
25	23.271622258339466	24.184670877207843	31.416354758387993	21.1273521060647
26	21.476936434239853	26.759524452373768	29.43020048599457	22.33333862739181
27	20.883119541605122	25.567831888937715	31.657057950528788	21.89199061892838
28	20.57924058495817	25.65747794583472	33.79850460494853	19.964776864258578
29	21.398407900147	25.583890532988164	32.71039911906867	20.30730244779617
30	22.9029793196429	24.882427784630643	32.25811005641702	19.95648283930944
31	26.696701625099482	24.38619803661018	28.52050477082903	20.396595567461304
32	26.72176016856282	24.617371923490268	28.57185713806728	20.089010769879632
33	25.939474794105244	24.578901765215566	28.16350876078444	21.318114679894755
34	22.038812507389625	25.861475665860205	30.163956991068925	21.935754835681248
35	22.736392946196485	25.373539942788874	30.072193310780644	21.817873800233997
36	26.415763588524598	26.081355561320198	26.799229890960042	20.703650959195162
37	22.556042020706826	29.050440024493845	27.040462488948652	21.353055465850677
38	22.91692034030208	29.110086416681224	26.032297286089157	21.940695956927538
39	22.562924296728447	27.39675262452949	26.466233612683155	23.574089466058908
40	23.987202495972106	25.237129702668028	27.98545192730199	22.79021587405788
41	21.964519220079303	24.866192671964253	28.37138879036058	24.797899317595864
42	22.992801845155846	27.023345033202574	25.953768751996304	24.03008436964528
43	22.447690290520285	25.603831486589268	27.639573440061554	24.308904782828897
44	22.581100564170164	26.26947110591117	29.148733043571866	22.0006952863468
45	22.21545759194456	28.014392780315983	27.487633961738073	22.28251566600138
46	23.351562541359833	27.23687205848876	28.44656442075059	20.96500097940082
47	22.40445547961523	27.34045913604495	28.190861396254984	22.064223988084837
48	22.31092711316756	26.68187826136061	29.146438951564658	21.86075567390717
49	22.469748867512656	25.840652369179402	29.826725466009496	21.862873297298442
50	21.476583497007976	27.46928122568042	29.1249097804201	21.929225496891505
51	21.89957876941375	28.14533249334272	28.16139113739317	21.793697599850354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	597.5
2	917.0
3	5306.5
4	9696.0
5	6979.5
6	4263.0
7	4147.5
8	4032.0
9	4051.0
10	4070.0
11	3989.5
12	3909.0
13	3776.0
14	3643.0
15	3584.0
16	3525.0
17	3371.5
18	3218.0
19	3130.5
20	3043.0
21	3112.5
22	3182.0
23	3419.5
24	3657.0
25	4146.0
26	5255.0
27	5875.0
28	6687.0
29	7499.0
30	8662.0
31	9825.0
32	11305.5
33	12786.0
34	14265.0
35	15744.0
36	17424.5
37	19105.0
38	21623.0
39	24141.0
40	27692.5
41	31244.0
42	32963.0
43	34682.0
44	35815.0
45	36948.0
46	38374.0
47	39800.0
48	40240.0
49	40680.0
50	39709.5
51	38739.0
52	36498.5
53	34258.0
54	32761.5
55	31265.0
56	30130.5
57	28996.0
58	27343.5
59	25691.0
60	23412.0
61	21133.0
62	18978.0
63	16823.0
64	14612.5
65	12402.0
66	10560.0
67	8718.0
68	7602.0
69	6486.0
70	5733.5
71	4981.0
72	4160.5
73	3340.0
74	2572.5
75	1308.5
76	812.0
77	633.5
78	455.0
79	332.5
80	210.0
81	151.5
82	93.0
83	60.0
84	27.0
85	25.5
86	24.0
87	16.5
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	566673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.69333160998995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.30317768056568	30.753064161641618
2	11.416395312007944	10.433062735646272
3	6.312135710108735	8.652675305077736
4	4.124126846863571	7.537803824615976
5	2.795876357871459	6.387645278037573
6	2.031700752506445	5.570110572992586
7	1.5475964562661897	4.950038665223142
8	1.0784114506384224	3.9420969620825392
9	0.8337371624160147	3.4286605774062306
>10	2.520636167670387	15.533658993987187
>50	0.021644953068644703	0.6951981885259614
>100	0.013774060826466202	1.2143490196189008
>500	0.0	0.0
>1k	7.870891900837826E-4	0.9016357151442568
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2598	0.4584654642095177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2416	0.42634817610861997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00635287017380394	0.0
2	0.0	0.0	0.0	0.018705673289533823	0.0
3	0.0	0.0	0.0	0.027882041318361736	0.0
4	0.0	0.0	0.0	0.03776428381094564	0.0
5	0.0	0.0	0.0	0.06670513682494136	0.0
6	0.0	0.0	0.0	0.13252793057018775	0.0
7	0.0	0.0	0.0	0.1706451516130114	0.0
8	0.0	0.0	0.0	0.46075955621672465	0.0
9	0.0	0.0	0.0	0.6271694610472001	0.0
10	0.0	0.0	0.0	0.8684020590358108	0.0
11	0.0	0.0	0.0	1.0120475124101553	0.0
12	0.0	0.0	0.0	1.1233992090676634	0.0
13	0.0	0.0	0.0	1.1841044129506788	0.0
14	0.0	0.0	0.0	1.2160452324356374	0.0
15	0.0	0.0	0.0	1.247986051920596	0.0
16	0.0	0.0	0.0	1.3016325111660516	0.0
17	0.0	0.0	0.0	1.367631773527237	0.0
18	0.0	0.0	0.0	1.4645130436777472	0.0
19	0.0	0.0	0.0	1.5103948838218868	0.0
20	0.0	0.0	0.0	1.5619237196760742	0.0
21	0.0	0.0	0.0	1.6250994841822357	0.0
22	0.0	0.0	0.0	1.688275248688397	0.0
23	0.0	0.0	0.0	1.7662743769334344	0.0
24	0.0	0.0	0.0	1.8257443005048768	0.0
25	0.0	0.0	0.0	1.8765672618953082	0.0
26	0.0	0.0	0.0	1.9298607839088857	0.0
27	0.0	0.0	0.0	1.9865072096253042	0.0
28	0.0	0.0	0.0	2.0410360119504545	0.0
29	0.0	0.0	0.0	2.105094119536311	0.0
30	0.0	0.0	0.0	2.2049753561577843	0.0
31	0.0	0.0	0.0	2.2803274551637362	0.0
32	0.0	0.0	0.0	2.357973646176896	0.0
33	0.0	0.0	0.0	2.437913929197262	0.0
34	0.0	0.0	0.0	2.518383618065445	0.0
35	0.0	0.0	0.0	2.6152648882159553	0.0
36	0.0	0.0	0.0	2.6976757318594675	0.0
37	0.0	0.0	0.0	2.7889100062999295	0.0
38	0.0	0.0	0.0	2.9006146401893154	0.0
39	0.0	0.0	0.0	3.0968477411134816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.163546E-6	45.000004	2
CGTTAAG	20	7.030255E-4	45.000004	2
CTTGCGG	20	7.030255E-4	45.000004	2
CATACGA	70	0.0	45.0	18
CGTTTTA	1365	0.0	44.67033	1
CGTTATT	300	0.0	42.75	1
CGTTTTT	1910	0.0	42.40838	1
TTCGAAT	75	0.0	42.0	16
AGGCGAT	450	0.0	41.5	7
GTTTTAT	1605	0.0	40.654205	2
CGTTCGA	80	0.0	39.375004	14
GCAAGGA	735	0.0	38.87755	4
GACCGAT	215	0.0	38.720932	9
GACACGA	70	0.0	38.571426	25
CGTAAAT	35	6.2426843E-6	38.571426	16
CGTTTAG	35	6.2426843E-6	38.571426	2
AGGTACC	170	0.0	38.382355	7
CGTTGAT	65	9.094947E-12	38.07692	25
GTAAGGC	95	0.0	37.894737	4
TTGCGGA	30	1.1391914E-4	37.500004	1
>>END_MODULE