FastQCFastQC Report
Thu 26 May 2016
SRR1545746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545746_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1817865
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97720.5375536687267757No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.23093023959424927No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA207.857691E-444.00000428
ACGTTCG454.802132E-1044.013
CACGACG1300.043.99999626
CGTTATT33900.043.3510321
CGTTTTA63750.043.3443151
TACGGGT1300.040.6153834
AGGCGAT14150.040.424037
CGTTTTT32900.040.3890571
CGTACAG501.3496901E-939.6000022
TAGGCGA4300.038.8837246
GTTTTAT74400.038.6774182
TACGGGA6450.038.5426374
TAAGGAT16650.038.3183175
GGCGATA6950.038.302168
CGTAAAG1150.038.2608682
GTACGGG4150.038.1686743
TCACGAC1500.038.1333325
TTACGGG5050.037.9009863
TAACGGG5400.037.888893
GCGCGAC3100.037.6129049
Produced by FastQC (version 0.11.5)