FastQCFastQC Report
Thu 26 May 2016
SRR1545745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545745_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences706299
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.38666343857204954No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.3328618616195124No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7230.10236457930706401No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCG207.031436E-445.00000443
CGCGAAG406.8084773E-945.0000042
TCGATAG406.8084773E-945.0000042
GCGTAAA207.031436E-445.0000041
GTACGAA207.031436E-445.0000041
GCGCGAA253.889273E-545.01
CGTTTTA16350.043.6238561
CGTTATT3500.042.428571
CGTTTTT24250.042.2164961
AGGCGAT5350.041.6355177
GCGATAT603.6379788E-1241.2499969
GACGGTC556.002665E-1140.90909229
ATAGCGG451.9264917E-840.02
TCGTCCC900.040.038
TATACGG451.9264917E-840.02
TAAGGAT6400.039.3750045
TCGAGGA403.455698E-739.3750044
GTTTTAT19400.038.6211362
GGCGATA3150.038.571438
ATTAGCG356.2448707E-638.5714261