##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545745_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	706299
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96145400177545	31.0	31.0	33.0	30.0	33.0
2	31.127278956929008	31.0	31.0	33.0	30.0	34.0
3	31.020263372877494	31.0	31.0	34.0	28.0	34.0
4	34.99419367718205	35.0	35.0	37.0	32.0	37.0
5	32.705504326071534	35.0	35.0	37.0	28.0	37.0
6	33.92628617625113	35.0	35.0	37.0	28.0	37.0
7	15.778667391572125	0.0	0.0	35.0	0.0	37.0
8	25.16104794145256	17.0	17.0	35.0	17.0	37.0
9	32.73644164865022	32.0	32.0	37.0	27.0	39.0
10	35.37718303438062	37.0	34.0	37.0	32.0	39.0
11	36.31047191062142	37.0	35.0	39.0	32.0	39.0
12	36.436353442380636	37.0	35.0	39.0	32.0	39.0
13	36.43063631691394	38.0	35.0	39.0	32.0	39.0
14	37.53551682785902	39.0	36.0	40.0	32.0	41.0
15	37.752604775031536	39.0	36.0	41.0	33.0	41.0
16	37.68332958138126	39.0	36.0	41.0	33.0	41.0
17	37.5974523537482	39.0	36.0	41.0	32.0	41.0
18	37.48454266535844	39.0	36.0	40.0	32.0	41.0
19	37.46796753216414	39.0	36.0	40.0	32.0	41.0
20	37.57616675090861	39.0	36.0	41.0	32.0	41.0
21	37.46173362839251	39.0	36.0	41.0	32.0	41.0
22	37.47778348829603	39.0	36.0	41.0	32.0	41.0
23	37.436931101417386	39.0	35.0	41.0	32.0	41.0
24	37.25139211580365	39.0	35.0	41.0	32.0	41.0
25	36.977079112387244	39.0	35.0	40.0	31.0	41.0
26	36.87457153415197	39.0	35.0	40.0	31.0	41.0
27	36.83315847820824	39.0	35.0	40.0	31.0	41.0
28	36.71197892110848	39.0	35.0	40.0	31.0	41.0
29	36.70435183965997	39.0	35.0	40.0	31.0	41.0
30	36.39110065283966	38.0	35.0	40.0	30.0	41.0
31	36.28346351899125	38.0	35.0	40.0	30.0	41.0
32	36.16650880151324	38.0	35.0	40.0	30.0	41.0
33	35.92042463602525	38.0	35.0	40.0	29.0	41.0
34	35.73220548238069	38.0	35.0	40.0	27.0	41.0
35	35.52525913246373	38.0	35.0	40.0	26.0	41.0
36	35.33517249776653	38.0	34.0	40.0	25.0	41.0
37	35.246467855681516	38.0	34.0	40.0	25.0	41.0
38	35.13343215833521	38.0	34.0	40.0	24.0	41.0
39	35.01787486602699	38.0	34.0	40.0	24.0	41.0
40	34.88460835991556	38.0	34.0	40.0	23.0	41.0
41	34.73823267483035	38.0	34.0	40.0	23.0	41.0
42	34.68085187717949	38.0	34.0	40.0	23.0	41.0
43	34.60837973719345	38.0	34.0	40.0	23.0	41.0
44	34.420105366140966	38.0	33.0	40.0	22.0	41.0
45	34.366295294202594	38.0	33.0	40.0	22.0	41.0
46	34.293057189660466	38.0	33.0	40.0	22.0	41.0
47	34.22660941046214	38.0	33.0	40.0	22.0	41.0
48	34.146704157870815	38.0	33.0	40.0	22.0	41.0
49	34.03499650997665	37.0	33.0	40.0	21.0	41.0
50	33.93498787340772	37.0	33.0	40.0	20.0	41.0
51	33.78905392758591	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	16.0
15	41.0
16	75.0
17	218.0
18	443.0
19	902.0
20	1600.0
21	2555.0
22	3905.0
23	5923.0
24	8396.0
25	11242.0
26	13062.0
27	13531.0
28	14143.0
29	15782.0
30	18850.0
31	24151.0
32	31326.0
33	43597.0
34	56177.0
35	67583.0
36	87448.0
37	135989.0
38	120265.0
39	29075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.949078223245394	22.90078281294466	27.252764056015938	15.897374907794006
2	35.45226596667984	23.035569921520487	29.2567312144007	12.255432897398977
3	30.174331267635946	22.817673534862713	34.45495462969649	12.553040567804855
4	26.227985598167347	27.324546686318403	31.712348452992288	14.735119262521962
5	20.542857911451097	36.322294099241255	30.01689086350115	13.117957125806493
6	19.92201603003827	40.98816506890142	29.320443608160286	9.769375292900033
7	38.507204455903235	55.23836222336433	5.493424173048525	0.7610091476839129
8	85.98794561510068	3.116243970329846	9.1114386400094	1.784371774560066
9	80.48701753789825	5.950595994047847	10.757200562368062	2.8051859056858355
10	35.72368076409566	36.7200010193983	17.207018557296553	10.349299659209485
11	29.548817144014077	25.25998196231341	30.45947962548439	14.731721268188119
12	28.461458957183854	24.422801108312488	31.668740859041282	15.446999075462376
13	21.960954213442182	30.171782771885564	31.339560157950103	16.527702856722154
14	17.934047761642024	33.306574127954306	31.85732954456965	16.902048565834015
15	17.387961755573773	27.24214532372267	39.58974881742718	15.780144103276376
16	19.619452951228872	24.94042891183479	38.311395032415454	17.12872310452089
17	20.44799723629794	24.169084198052097	31.681341754695957	23.701576810954002
18	21.02084244774522	25.890026745047066	34.17263793379291	18.9164928734148
19	24.856894884461113	26.172485024047887	29.96903577663284	19.001584314858157
20	26.56721869916282	25.845852818707094	29.68417058497888	17.902757897151204
21	23.61620220331616	26.91905269581296	31.40213988693174	18.06260521393914
22	22.094183907948334	25.315340953335625	30.93250875337499	21.65796638534105
23	20.29225582933007	28.27173760687754	30.94029582372338	20.495710740069008
24	19.594675909211254	26.31505920297211	35.783995163521396	18.306269724295234
25	19.56466029259563	27.788939245277145	33.111331036855496	19.535069425271733
26	19.13778725440642	30.878424010227963	30.693516485227928	19.29027225013769
27	19.58235817975107	29.64339465297275	31.739532407663045	19.034714759613138
28	17.7969953235103	28.445460067195338	35.26750002477704	18.490044584517324
29	18.890158417327505	26.084278754465178	34.72821000737648	20.297352820830838
30	19.624408359632394	28.42351468712259	32.61451594862799	19.337561004617022
31	23.01631462029537	27.951335057815456	29.711496122746883	19.32085419914229
32	24.003573557374427	27.592846655594865	29.825895265319645	18.57768452171106
33	22.727909851210324	28.294390902436504	29.855769298838027	19.121929947515145
34	20.190882331703712	27.49388007062165	31.13114983880764	21.184087758866994
35	20.30754680383237	27.110189877091713	31.5679336938039	21.014329625272016
36	23.119103948894164	28.11939419424351	29.38443916811435	19.377062688747966
37	20.642815578105022	30.19910830965356	29.8159844485126	19.342091663728816
38	20.03740625429174	30.53720874587108	29.194434651613555	20.23095034822363
39	20.871047530861574	29.848406977781366	29.014482535017038	20.26606295634002
40	21.974404607680317	27.93533616782694	29.54839239472235	20.541866829770395
41	19.507460721309247	28.023684020506895	29.03741899677049	23.431436261413367
42	20.984597174850876	28.347909313194553	27.879977176804726	22.787516335149842
43	21.32099861390148	28.247810063443385	28.417568197038364	22.01362312561677
44	20.57188244638602	29.658260878183317	28.977387763539237	20.792468911891422
45	19.40339714483526	31.18098708903736	27.83028150967225	21.585334256455127
46	20.985305090337096	30.744911149527326	28.390101076173124	19.879682683962457
47	20.903470060130342	28.71914019416706	29.05879804445426	21.318591701248337
48	20.942405411872308	27.174893352532003	30.45183413823324	21.430867097362448
49	20.632338428909	27.184379420047318	30.98093017263227	21.20235197841141
50	19.489762834153808	30.14474040031205	29.51228870492525	20.853208060608893
51	19.345631241159904	30.18863116045754	28.59199857284238	21.873739025540175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	309.0
1	526.0
2	743.0
3	5240.0
4	9737.0
5	7270.5
6	4804.0
7	4814.0
8	4824.0
9	4819.0
10	4814.0
11	4837.0
12	4860.0
13	4702.5
14	4545.0
15	4462.5
16	4380.0
17	4365.0
18	4350.0
19	4291.5
20	4233.0
21	4561.5
22	4890.0
23	5400.5
24	5911.0
25	6909.0
26	9500.0
27	11093.0
28	12929.0
29	14765.0
30	17042.5
31	19320.0
32	21773.0
33	24226.0
34	26823.5
35	29421.0
36	31866.5
37	34312.0
38	37989.0
39	41666.0
40	45937.0
41	50208.0
42	53257.5
43	56307.0
44	56680.0
45	57053.0
46	57210.5
47	57368.0
48	54728.5
49	52089.0
50	48451.5
51	44814.0
52	40521.5
53	36229.0
54	33258.5
55	30288.0
56	27160.5
57	24033.0
58	21386.0
59	18739.0
60	16392.5
61	14046.0
62	11558.5
63	9071.0
64	7296.0
65	5521.0
66	4395.5
67	3270.0
68	2732.5
69	2195.0
70	1918.0
71	1641.0
72	1333.5
73	1026.0
74	810.5
75	466.0
76	337.0
77	270.0
78	203.0
79	146.0
80	89.0
81	65.0
82	41.0
83	27.5
84	14.0
85	10.0
86	6.0
87	5.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	706299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.8355204470559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.52402443491536	27.57378680479488
2	9.770009355930622	7.979268336440646
3	5.520595330663886	6.763091505157391
4	3.74271546901861	6.113429362504873
5	2.8024050075411298	5.721883349318882
6	2.138795508499627	5.24032966316447
7	1.6628445010365798	4.7532184435647595
8	1.307132988707084	4.270196470989553
9	1.0654616696569932	3.9157813617149215
>10	4.445318221079515	26.225774203752593
>50	0.01641527160377851	0.41603934135626636
>100	0.003211681010112348	0.18127936439327974
>500	3.5685344556775253E-4	0.10535770133139953
>1k	7.137068911355051E-4	0.7405640915161443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2731	0.38666343857204954	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2351	0.3328618616195124	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	723	0.10236457930706401	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01585730689127409	0.0
2	0.0	0.0	0.0	0.06838463596861952	0.0
3	0.0	0.0	0.0	0.09033001604136492	0.0
4	0.0	0.0	0.0	0.12317729460186125	0.0
5	0.0	0.0	0.0	0.20954298392040765	0.0
6	0.0	0.0	0.0	0.3405073488706624	0.0
7	0.0	0.0	0.0	0.41568797350697084	0.0
8	0.0	0.0	0.0	0.8650727241578992	0.0
9	0.0	0.0	0.0	1.0587584011870328	0.0
10	0.0	0.0	0.0	1.3519769955783598	0.0
11	0.0	0.0	0.0	1.5858722722246528	0.0
12	0.0	0.0	0.0	1.7855044393380142	0.0
13	0.0	0.0	0.0	1.887585852450591	0.0
14	0.0	0.0	0.0	1.9355825224161438	0.0
15	0.0	0.0	0.0	1.9902335979521422	0.0
16	0.0	0.0	0.0	2.1067564869835578	0.0
17	0.0	0.0	0.0	2.256268237672714	0.0
18	0.0	0.0	0.0	2.4631211427454947	0.0
19	0.0	0.0	0.0	2.5681758009001854	0.0
20	0.0	0.0	0.0	2.677336368874938	0.0
21	0.0	0.0	0.0	2.806601736658271	0.0
22	0.0	0.0	0.0	2.952998659208069	0.0
23	0.0	0.0	0.0	3.10916481546767	0.0
24	0.0	0.0	0.0	3.229793614319148	0.0
25	0.0	0.0	0.0	3.330176030264803	0.0
26	0.0	0.0	0.0	3.4390534320450685	0.0
27	0.0	0.0	0.0	3.545807087366682	0.0
28	0.0	0.0	0.0	3.658082483480792	0.0
29	0.0	0.0	0.0	3.7838082738330368	0.0
30	0.0	0.0	0.0	3.9503099961914145	0.0
31	0.0	0.0	0.0	4.0924594258239075	0.0
32	0.0	0.0	0.0	4.223706956968649	0.0
33	0.0	0.0	0.0	4.352405992363008	0.0
34	0.0	0.0	0.0	4.492290092439604	0.0
35	0.0	0.0	0.0	4.640810761448055	0.0
36	0.0	0.0	0.0	4.7831017741777915	0.0
37	0.0	0.0	0.0	4.919871046115031	0.0
38	0.0	0.0	0.0	5.063011557428228	0.0
39	1.415830972435187E-4	0.0	0.0	5.2149302207705235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACCG	20	7.031436E-4	45.000004	43
CGCGAAG	40	6.8084773E-9	45.000004	2
TCGATAG	40	6.8084773E-9	45.000004	2
GCGTAAA	20	7.031436E-4	45.000004	1
GTACGAA	20	7.031436E-4	45.000004	1
GCGCGAA	25	3.889273E-5	45.0	1
CGTTTTA	1635	0.0	43.623856	1
CGTTATT	350	0.0	42.42857	1
CGTTTTT	2425	0.0	42.216496	1
AGGCGAT	535	0.0	41.635517	7
GCGATAT	60	3.6379788E-12	41.249996	9
GACGGTC	55	6.002665E-11	40.909092	29
ATAGCGG	45	1.9264917E-8	40.0	2
TCGTCCC	90	0.0	40.0	38
TATACGG	45	1.9264917E-8	40.0	2
TAAGGAT	640	0.0	39.375004	5
TCGAGGA	40	3.455698E-7	39.375004	4
GTTTTAT	1940	0.0	38.621136	2
GGCGATA	315	0.0	38.57143	8
ATTAGCG	35	6.2448707E-6	38.571426	1
>>END_MODULE