FastQCFastQC Report
Thu 26 May 2016
SRR1545744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545744_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1322512
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68110.5150047787846159No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.22752156502171625No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.21905283279093118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCG207.856912E-444.00000423
GACGATA207.856912E-444.0000041
CCGGATA254.443204E-544.020
TAGTACG454.802132E-1043.9999961
CGTTTTA35500.043.6281661
CGTTATT19650.042.432571
CGACGGT4300.041.4418628
TCACGAC4300.040.93023325
CACGACG4300.040.93023326
CGTTCGA603.6379788E-1240.33333614
GTTATTT22400.040.2678572
AGTACGG1150.040.1739162
AGGCGAT9050.040.1104977
CGGTCTA4450.040.04494531
ACGACGG4400.040.00000427
GTTTTAT42850.039.4305732
TAACGGG3800.039.368423
CGAATAT950.039.3684214
ACGGTCT4500.039.1111130
ATTAACG452.3514076E-839.1111071
Produced by FastQC (version 0.11.5)