##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545744_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1322512
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46072701041654	31.0	31.0	33.0	30.0	34.0
2	31.654158903662122	31.0	31.0	34.0	30.0	34.0
3	31.707849153731686	31.0	31.0	34.0	30.0	34.0
4	35.623634416927786	37.0	35.0	37.0	33.0	37.0
5	27.066458376181085	35.0	25.0	37.0	0.0	37.0
6	31.30511556794948	35.0	26.0	37.0	17.0	37.0
7	15.026413370918373	0.0	0.0	32.0	0.0	37.0
8	25.103618719527688	17.0	17.0	35.0	17.0	37.0
9	33.11519517403245	32.0	32.0	37.0	28.0	39.0
10	35.910581529695	37.0	35.0	39.0	32.0	39.0
11	36.90385418052917	38.0	37.0	39.0	34.0	39.0
12	37.032873803791574	39.0	37.0	39.0	33.0	39.0
13	36.657866998560316	39.0	35.0	39.0	33.0	39.0
14	37.95503405640176	40.0	37.0	41.0	33.0	41.0
15	38.18872721003666	40.0	37.0	41.0	33.0	41.0
16	38.24193882550782	40.0	37.0	41.0	33.0	41.0
17	38.24992060563534	40.0	37.0	41.0	34.0	41.0
18	38.22845690625113	40.0	37.0	41.0	34.0	41.0
19	38.177277030378555	40.0	37.0	41.0	34.0	41.0
20	38.210742133152664	40.0	37.0	41.0	34.0	41.0
21	38.097490986849266	40.0	36.0	41.0	34.0	41.0
22	38.00569597856201	40.0	36.0	41.0	34.0	41.0
23	37.902575553189685	40.0	36.0	41.0	33.0	41.0
24	37.74363559650121	40.0	35.0	41.0	33.0	41.0
25	37.56276011106137	39.0	35.0	41.0	33.0	41.0
26	37.318901454202305	39.0	35.0	40.0	33.0	41.0
27	37.12726689814535	39.0	35.0	40.0	32.0	41.0
28	37.12582948207653	39.0	35.0	40.0	32.0	41.0
29	37.01815786926697	39.0	35.0	40.0	32.0	41.0
30	36.79780750571639	39.0	35.0	40.0	31.0	41.0
31	36.64080325925209	38.0	35.0	40.0	31.0	41.0
32	36.45251612083671	38.0	35.0	40.0	31.0	41.0
33	36.24027759294434	38.0	35.0	40.0	30.0	41.0
34	35.97310799448323	38.0	35.0	40.0	30.0	41.0
35	35.76364978162769	38.0	35.0	40.0	29.0	41.0
36	35.581273364627314	38.0	35.0	40.0	28.0	41.0
37	35.492018219872485	38.0	35.0	40.0	28.0	41.0
38	35.294342886869835	38.0	34.0	40.0	27.0	41.0
39	35.2160827274157	38.0	34.0	40.0	27.0	41.0
40	34.96342490654149	38.0	34.0	40.0	26.0	41.0
41	34.728003224167345	38.0	34.0	40.0	25.0	40.0
42	34.73141037661662	38.0	34.0	40.0	25.0	40.0
43	34.60961790894903	37.0	34.0	40.0	24.0	40.0
44	34.372515334454434	37.0	33.0	40.0	24.0	40.0
45	34.15098312907558	37.0	33.0	40.0	23.0	40.0
46	34.09879985966101	36.0	33.0	39.0	23.0	40.0
47	33.93070686693202	36.0	33.0	39.0	23.0	40.0
48	33.82164623080925	36.0	33.0	39.0	23.0	40.0
49	33.66459283545253	36.0	33.0	39.0	23.0	40.0
50	33.76750532320312	36.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	2.0
14	12.0
15	36.0
16	87.0
17	233.0
18	494.0
19	1008.0
20	1895.0
21	3157.0
22	5027.0
23	7493.0
24	11533.0
25	16600.0
26	21189.0
27	22759.0
28	24343.0
29	27135.0
30	32944.0
31	43550.0
32	59141.0
33	96626.0
34	107308.0
35	138979.0
36	191606.0
37	331585.0
38	140163.0
39	37601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.15454831411738	23.233437579394366	27.28375999612858	15.32825411035968
2	34.75159393638772	23.483189566521894	29.248732714712606	12.516483782377778
3	30.918963306193064	22.987768731020967	33.243781530904826	12.849486431881147
4	27.61895544237028	26.225773376725503	30.938849704199285	15.216421476704936
5	17.337007150029642	47.407584959531555	23.670635880808643	11.584772009630157
6	19.701598170753837	41.376562178641855	28.610704477539713	10.311135173064592
7	38.91042198482887	55.5741649225111	4.427029773642886	1.0883833190171432
8	86.16080610232648	2.940086744014421	8.84271749519097	2.0563896584681274
9	81.11170257812405	4.943319984998245	10.602247843497828	3.342729593379871
10	43.94667118332386	25.102910219340163	17.408764532949416	13.54165406438656
11	36.494338047594276	23.333474478870514	23.687119663186422	16.48506781034879
12	33.25701392501543	21.220525787289642	29.347862250021173	16.17459803767376
13	23.953355432691726	29.03444354380149	29.44305987393687	17.56914114956991
14	19.363907473051285	32.3005008650205	30.829814776727925	17.505776885200287
15	17.68656919559142	23.635399905634127	40.548970444124514	18.129060454649938
16	20.454483588806756	18.846407442805813	39.37174105036476	21.32736791802267
17	20.13819156272306	19.815850442188804	30.725619124817015	29.320338870271122
18	22.474503066890886	23.45899318871965	33.149491271156705	20.917012473232756
19	27.325725588879347	24.741325598557896	26.949849982457625	20.98309883010513
20	28.094943561948778	23.75479390735207	26.481952526706753	21.668310003992403
21	24.322047739453403	25.263513677002553	29.89560775251945	20.518830831024594
22	23.596080791705482	22.634426001427585	28.23467764375673	25.534815563110204
23	21.998514947312387	26.805427852450485	27.528143411931232	23.667913788305892
24	21.537876404902185	24.902836420387867	34.01874614370229	19.540541031007656
25	22.765918192046648	23.824736561936678	31.37929939388074	22.030045852135935
26	19.94787192857229	27.779634513713297	28.225074706316466	24.047418851397946
27	20.34779268543499	26.764672078589836	30.640629347786636	22.24690588818854
28	19.50901012618411	26.084905089708073	33.867896850841426	20.538187933266393
29	20.05652878764049	25.07122808715535	33.01179875872582	21.860444366478337
30	22.098551846788535	25.422680474732935	31.81869049203334	20.66007718644519
31	27.380696734698816	24.55032544128144	27.030832234414508	21.038145589605236
32	26.8446713526985	25.149639473970748	27.403910134652843	20.601779038677908
33	26.16921434361276	24.452405724862988	27.06395102653133	22.314428904992923
34	21.2438148009243	26.24482802424477	29.333041968617295	23.17831520621363
35	22.04335385992717	24.82238346419541	29.697121840860426	23.437140835016997
36	27.428787035580775	25.501923612035277	26.082561065608477	20.98672828677547
37	21.80025587669526	29.788085098660734	27.024253844199524	21.38740518044449
38	23.14194502582963	29.404950578898337	25.522036851083392	21.931067544188636
39	22.127889954873755	26.99204241625029	25.94161716491041	24.938450463965545
40	23.6903710514536	24.63418101310234	28.136304245254486	23.53914369018958
41	21.039431022175982	23.73944433018377	28.29116106318884	26.929963584451404
42	22.53045719055857	26.43023276915446	25.69163833674099	25.347671703545977
43	22.435259566642873	25.549484617152814	26.84270539700207	25.172550419202246
44	21.99034866980413	26.604371075649976	28.750211718305767	22.655068536240126
45	20.98718196885926	29.3538357307911	26.433030475337844	23.225951825011794
46	22.934158631452874	27.271208125143666	28.140538611369877	21.654094632033587
47	22.53068403160047	27.25888309520065	27.841713345512176	22.368719527686707
48	22.336432486056836	26.20029156635252	28.66325598557896	22.800019962011685
49	22.206830637453574	25.984641349190024	29.67814280702179	22.130385206334612
50	20.63792237802001	27.149318871964866	29.514287961092222	22.698470788922897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	723.0
1	1455.5
2	2188.0
3	11387.0
4	20586.0
5	14437.5
6	8289.0
7	8201.0
8	8113.0
9	8150.0
10	8187.0
11	8089.0
12	7991.0
13	7561.0
14	7131.0
15	6664.5
16	6198.0
17	5908.5
18	5619.0
19	5745.5
20	5872.0
21	5889.0
22	5906.0
23	6379.5
24	6853.0
25	8186.5
26	9520.0
27	11467.5
28	13415.0
29	15865.0
30	18315.0
31	21868.5
32	25422.0
33	28626.0
34	31830.0
35	33940.0
36	36050.0
37	40376.0
38	44702.0
39	55090.0
40	65478.0
41	75873.5
42	86269.0
43	90181.5
44	94094.0
45	98366.0
46	102638.0
47	105981.5
48	109325.0
49	104047.0
50	98769.0
51	90842.5
52	82916.0
53	78301.5
54	73687.0
55	72812.5
56	71938.0
57	69256.5
58	66575.0
59	60559.0
60	54543.0
61	49076.0
62	43609.0
63	38293.0
64	32977.0
65	28228.0
66	23479.0
67	19966.0
68	16453.0
69	13837.5
70	11222.0
71	9402.0
72	7582.0
73	5884.0
74	4186.0
75	3086.5
76	1987.0
77	1552.5
78	1118.0
79	762.5
80	407.0
81	291.5
82	176.0
83	131.5
84	87.0
85	65.5
86	44.0
87	36.5
88	29.0
89	20.5
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1322512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.1893314419368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18402289645462	20.486247062530953
2	7.71653885535573	4.50481220467124
3	3.8331189244211568	3.356585362238683
4	2.66866399575604	3.1158607151714586
5	2.0149021252066563	2.940682297785997
6	1.5916373973005136	2.78752989151118
7	1.334122083728974	2.7259492180180667
8	1.121660026252444	2.61924050171633
9	0.9439519276484087	2.4797993113246095
>10	8.324473788883383	46.81042742783757
>50	0.22017791742456205	3.9752716253678155
>100	0.04193723474427294	2.327663883710514
>500	0.0035946201174601932	0.6614619011617177
>1k	8.986550293650483E-4	0.6129342930791329
>5k	2.995516764550161E-4	0.5955343038747467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6811	0.5150047787846159	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3009	0.22752156502171625	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2897	0.21905283279093118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011946961539857484	0.0
2	0.0	0.0	0.0	0.04279734323771731	0.0
3	0.0	0.0	0.0	0.059810421379919425	0.0
4	0.0	0.0	0.0	0.07999927410866593	0.0
5	0.0	0.0	0.0	0.13247516846728044	0.0
6	0.0	0.0	0.0	0.24597130309592655	0.0
7	0.0	0.0	0.0	0.3201483237959277	0.0
8	0.0	0.0	0.0	0.6805987393687165	0.0
9	0.0	0.0	0.0	0.8364385351512879	0.0
10	0.0	0.0	0.0	1.0855099991531267	0.0
11	0.0	0.0	0.0	1.30463844562469	0.0
12	0.0	0.0	0.0	1.4902700315762731	0.0
13	0.0	0.0	0.0	1.5816869714603723	0.0
14	0.0	0.0	0.0	1.6242574736561937	0.0
15	0.0	0.0	0.0	1.6724990018994157	0.0
16	0.0	0.0	0.0	1.773216424501252	0.0
17	0.0	0.0	0.0	1.8883004464231705	0.0
18	0.0	0.0	0.0	2.0666731190340806	0.0
19	0.0	0.0	0.0	2.1391110250795458	0.0
20	0.0	0.0	0.0	2.223873961068028	0.0
21	0.0	0.0	0.0	2.327086635130721	0.0
22	0.0	0.0	0.0	2.447236773654984	0.0
23	0.0	0.0	0.0	2.5759312580906637	0.0
24	0.0	0.0	0.0	2.6802781373628366	0.0
25	0.0	0.0013610462513761689	0.0	2.7645873912675274	0.0
26	0.0	0.0013610462513761689	0.0	2.847686826282106	0.0
27	0.0	0.0013610462513761689	0.0	2.94757249839699	0.0
28	0.0	0.0013610462513761689	0.0	3.0405773255743616	0.0
29	0.0	0.0014366599320081784	0.0	3.157098007428288	0.0
30	7.56136806320094E-5	0.0014366599320081784	0.0	3.28549003714144	0.0
31	7.56136806320094E-5	0.0014366599320081784	0.0	3.4027668558016866	0.0
32	7.56136806320094E-5	0.0014366599320081784	0.0	3.5076430308382833	0.0
33	7.56136806320094E-5	0.0014366599320081784	0.0	3.617509708796593	0.0
34	7.56136806320094E-5	0.0014366599320081784	0.0	3.735315823221264	0.0
35	7.56136806320094E-5	0.0014366599320081784	0.0	3.885408979275802	0.0
36	7.56136806320094E-5	0.0014366599320081784	0.0	4.002383343213521	0.0
37	7.56136806320094E-5	0.0014366599320081784	0.0	4.120567526041351	0.0
38	7.56136806320094E-5	0.0014366599320081784	0.0	4.261057744655625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCCG	20	7.856912E-4	44.000004	23
GACGATA	20	7.856912E-4	44.000004	1
CCGGATA	25	4.443204E-5	44.0	20
TAGTACG	45	4.802132E-10	43.999996	1
CGTTTTA	3550	0.0	43.628166	1
CGTTATT	1965	0.0	42.43257	1
CGACGGT	430	0.0	41.44186	28
TCACGAC	430	0.0	40.930233	25
CACGACG	430	0.0	40.930233	26
CGTTCGA	60	3.6379788E-12	40.333336	14
GTTATTT	2240	0.0	40.267857	2
AGTACGG	115	0.0	40.173916	2
AGGCGAT	905	0.0	40.110497	7
CGGTCTA	445	0.0	40.044945	31
ACGACGG	440	0.0	40.000004	27
GTTTTAT	4285	0.0	39.430573	2
TAACGGG	380	0.0	39.36842	3
CGAATAT	95	0.0	39.36842	14
ACGGTCT	450	0.0	39.11111	30
ATTAACG	45	2.3514076E-8	39.111107	1
>>END_MODULE