FastQCFastQC Report
Thu 26 May 2016
SRR1545743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545743_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences505262
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.3384382755877149No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.33685493862590105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA253.8876908E-545.00000420
ACCGGGT207.0295297E-445.04
CGTTCGA207.0295297E-445.014
TAGCCCG351.2101191E-745.011
TGCGTAA406.8012014E-945.01
CGTTTTA8050.044.161491
GTACGGG850.042.352943
AGGCGAT3250.042.230777
TACGGGA800.042.18754
ATTCGGC556.002665E-1140.90909217
CCGCTCG556.002665E-1140.90909219
CGTTTTT13100.040.706111
TACGAAG501.0786607E-940.5000042
CGTAAAG501.0786607E-940.5000042
TACCTGG3150.040.010
CATATGC3100.039.91935333
TATGGGC850.039.7058834
CGACGGT1250.039.628
CGGTCTA1250.039.631
ACGACGG1250.039.627