##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545743_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	505262
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.931823489595498	31.0	31.0	33.0	30.0	33.0
2	31.097375223151552	31.0	31.0	33.0	30.0	34.0
3	31.043553641477097	31.0	31.0	34.0	28.0	34.0
4	35.029066108276496	35.0	35.0	37.0	32.0	37.0
5	32.708272143957	35.0	35.0	37.0	28.0	37.0
6	33.91651064200355	35.0	35.0	37.0	28.0	37.0
7	15.41309261333724	0.0	0.0	35.0	0.0	37.0
8	24.94874144503248	17.0	17.0	35.0	17.0	37.0
9	32.611071087871245	32.0	32.0	37.0	27.0	39.0
10	35.338891505793036	37.0	34.0	37.0	32.0	39.0
11	36.315804473718586	37.0	35.0	39.0	32.0	39.0
12	36.30825393558194	37.0	35.0	39.0	32.0	39.0
13	36.14349782884919	37.0	35.0	39.0	32.0	39.0
14	37.15962609497647	39.0	36.0	40.0	32.0	41.0
15	37.42094992301024	39.0	36.0	41.0	32.0	41.0
16	37.41692033044242	39.0	36.0	41.0	32.0	41.0
17	37.35269820410005	39.0	36.0	41.0	32.0	41.0
18	37.29092233336368	39.0	36.0	40.0	32.0	41.0
19	37.25476287549826	39.0	36.0	40.0	32.0	41.0
20	37.369459805012056	39.0	35.0	40.0	32.0	41.0
21	37.25251255784128	39.0	35.0	40.0	32.0	41.0
22	37.29875787215346	39.0	35.0	40.0	32.0	41.0
23	37.27212614445575	39.0	35.0	40.0	32.0	41.0
24	37.084726339997864	39.0	35.0	40.0	32.0	41.0
25	36.82448907695414	39.0	35.0	40.0	31.0	41.0
26	36.70243556808151	38.0	35.0	40.0	31.0	41.0
27	36.621889237662835	38.0	35.0	40.0	31.0	41.0
28	36.49252071202663	38.0	35.0	40.0	30.0	41.0
29	36.44000142500327	38.0	35.0	40.0	30.0	41.0
30	36.130197798369956	38.0	35.0	40.0	30.0	41.0
31	36.02241609303688	38.0	35.0	40.0	30.0	41.0
32	35.94573310480503	38.0	35.0	40.0	29.0	41.0
33	35.760154137853235	38.0	34.0	40.0	29.0	41.0
34	35.546461043973224	38.0	34.0	40.0	27.0	41.0
35	35.36620208921312	38.0	34.0	40.0	26.0	41.0
36	35.147683775941985	38.0	34.0	40.0	25.0	41.0
37	35.023162240580135	38.0	34.0	40.0	25.0	41.0
38	34.87563679833433	38.0	34.0	40.0	24.0	41.0
39	34.76999853541331	38.0	34.0	40.0	24.0	41.0
40	34.60552940850489	38.0	33.0	40.0	23.0	41.0
41	34.41978814951451	38.0	33.0	40.0	23.0	41.0
42	34.38061243473683	37.0	33.0	40.0	23.0	41.0
43	34.29449473738377	37.0	33.0	40.0	23.0	41.0
44	34.051941369032306	37.0	33.0	40.0	22.0	41.0
45	34.00683803650384	37.0	33.0	40.0	23.0	41.0
46	33.96099449394571	37.0	33.0	40.0	23.0	41.0
47	33.87327366791882	37.0	33.0	40.0	22.0	41.0
48	33.79200098166891	36.0	33.0	40.0	22.0	41.0
49	33.640833864410936	36.0	32.0	40.0	22.0	41.0
50	33.515722536030815	36.0	32.0	40.0	21.0	41.0
51	33.35716915184597	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	12.0
15	25.0
16	75.0
17	186.0
18	400.0
19	683.0
20	1161.0
21	1960.0
22	2807.0
23	4078.0
24	5875.0
25	7699.0
26	9110.0
27	10246.0
28	11036.0
29	12660.0
30	15110.0
31	19429.0
32	25107.0
33	35311.0
34	44533.0
35	50795.0
36	63198.0
37	91047.0
38	75999.0
39	16716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.73635856248837	23.985575800277875	27.518990147685756	14.759075489547996
2	34.390078810597274	23.404491135292186	29.673120084233528	12.532309969877014
3	31.567384841923595	22.723854158832445	32.79170014764617	12.917060851597784
4	28.204970886391617	26.843499016351913	29.404150717845397	15.547379379411078
5	21.248382027542146	35.854863417395336	28.613471822539594	14.283282732522927
6	19.7562848581528	42.09519021814425	28.11986652469412	10.028658399008831
7	38.09211854443833	56.35650415032201	4.678760722159988	0.8726165830796696
8	86.82327188666474	3.234163661625058	8.00594543029161	1.9366190214185908
9	81.64694752425474	5.079938724859578	9.833314201345045	3.439799549540634
10	43.95145488875079	25.822840427342648	16.817611457026256	13.408093226880311
11	36.75301130898425	23.812002485839027	23.051209075687463	16.383777129489253
12	33.48163922875656	21.70161223286137	28.688086576865075	16.128661961516997
13	24.173003313132593	29.780193246276188	28.637023959846573	17.409779480744643
14	19.297117139226778	33.21821154173478	30.116850267781864	17.367821051256577
15	17.626300810272692	24.242868056572632	40.07920643151474	18.05162470163994
16	20.44483851942161	19.213398197370868	38.967308050080945	21.374455233126575
17	19.98111870673037	20.125004453135205	30.241340136404478	29.652536703729947
18	22.447957693236383	23.869794284945236	32.92846087772284	20.75378714409554
19	27.688209285479616	24.97793224109472	26.366122922365033	20.96773555106064
20	28.350439969758263	24.277701469732534	25.828975858069676	21.542882702439524
21	24.518368687928245	25.832340449113527	29.222462801477256	20.426828061480975
22	23.447439150381385	23.073771627393313	27.613990365394585	25.864798856830713
23	22.014915034180287	27.280697934932768	27.014895242468263	23.68949178841868
24	21.329132212594654	25.358131029050273	33.86540052487621	19.447336233478868
25	22.720489567788594	24.231190946479252	31.097331681385104	21.95098780434705
26	19.87305595908657	28.39378381908792	27.75668860907806	23.976471612747446
27	20.26948395090072	27.55619856628838	30.082808523102866	22.091508959708033
28	19.37984649548155	26.78926972540979	33.550118552355016	20.280765226753644
29	19.898389350475597	25.681131769260308	32.72143956996568	21.69903931029842
30	22.076863092811255	26.181070414953034	31.294457133130933	20.44760935910478
31	27.777469906701867	24.90391123812992	26.60184221255507	20.716776642613137
32	27.30345840375884	25.391381105248367	26.9388950683012	20.366265422691594
33	26.58046716357058	24.676702384109632	26.545237916170223	22.197592536149564
34	21.349715593098235	26.42668556115441	28.804066009317935	23.419532836429415
35	22.093290213790073	24.99297394223195	29.55159897241431	23.362136871563663
36	27.79369911056046	25.78088199785458	25.705277657927965	20.720141233656992
37	21.77464365022503	30.34247578484034	26.688134076973924	21.194746487960703
38	22.944729665005482	30.05292303794863	25.173870190119185	21.8284771069267
39	21.98641496886764	27.56827151062221	25.652037952586976	24.793275567923175
40	23.86108593165526	24.78041095510844	27.907303537570606	23.451199575665694
41	21.018996085199362	24.105513575135276	27.966084922277943	26.909405417387415
42	22.320301150690135	26.571956727400835	25.440464551064597	25.667277570844433
43	22.289623997054996	25.743475662131726	26.62856102378568	25.338339317027604
44	21.899727270208327	26.708915374597737	28.81752437349336	22.573832981700583
45	20.952100098562727	29.86668302781527	26.069049324904704	23.1121675487173
46	22.825781475749217	27.874053461372515	27.81507415954495	21.48509090333332
47	22.4932807137683	27.619532044760938	27.448729570005266	22.438457671465496
48	22.087154783063045	26.571758810280606	28.732420011795863	22.60866639486049
49	22.22074883921609	26.399966749923802	29.581088623328096	21.798195787532013
50	20.579620078296013	27.828532523720366	29.141514699304523	22.4503326986791
51	20.93369380638164	29.046118647355236	27.396479450265403	22.62370809599772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	539.0
2	800.0
3	3621.5
4	6443.0
5	4577.0
6	2711.0
7	2710.5
8	2710.0
9	2715.5
10	2721.0
11	2655.0
12	2589.0
13	2550.0
14	2511.0
15	2346.5
16	2182.0
17	2127.0
18	2072.0
19	2072.0
20	2072.0
21	2138.5
22	2205.0
23	2446.5
24	2688.0
25	2902.5
26	3889.0
27	4661.0
28	5565.5
29	6470.0
30	7934.5
31	9399.0
32	10769.0
33	12139.0
34	12900.0
35	13661.0
36	14997.5
37	16334.0
38	19628.5
39	22923.0
40	26795.5
41	30668.0
42	32481.5
43	34295.0
44	35827.5
45	37360.0
46	38623.0
47	39886.0
48	38615.5
49	37345.0
50	35862.5
51	34380.0
52	32211.0
53	30042.0
54	28720.0
55	27398.0
56	26636.5
57	25875.0
58	24317.5
59	22760.0
60	20560.0
61	18360.0
62	16055.0
63	13750.0
64	12149.5
65	10549.0
66	9068.0
67	7587.0
68	6423.0
69	5259.0
70	4495.5
71	3732.0
72	3143.0
73	2554.0
74	1984.5
75	1034.0
76	653.0
77	510.0
78	367.0
79	278.5
80	190.0
81	132.5
82	75.0
83	56.0
84	37.0
85	28.5
86	20.0
87	17.0
88	14.0
89	9.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05574358643023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52291522158606	28.13259237132134
2	9.853306979966163	8.09069689695748
3	5.332424206707291	6.567799227739446
4	3.589025968185196	5.894005194994036
5	2.6025176622312336	5.342414890986069
6	1.94094531345611	4.781217186272251
7	1.542137719670253	4.431952755562089
8	1.212673368477704	3.982976549625059
9	0.9907280821875218	3.6607570295550005
>10	4.362636012646442	26.009261095866815
>50	0.02773574493831414	0.7959201650950651
>100	0.021997314949867293	1.6405300688840705
>500	0.0	0.0
>1k	9.564049978203171E-4	0.6698765671412696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1710	0.3384382755877149	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1702	0.33685493862590105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008312519049522822	0.0
2	0.0	0.0	0.0	0.04472926917124185	0.0
3	0.0	0.0	0.0	0.060562638789380556	0.0
4	0.0	0.0	0.0	0.08055226793228068	0.0
5	0.0	0.0	0.0	0.1432919950441553	0.0
6	0.0	0.0	0.0	0.22562551705847658	0.0
7	0.0	0.0	0.0	0.2778756367983343	0.0
8	0.0	0.0	0.0	0.5777200739418361	0.0
9	0.0	0.0	0.0	0.7077516219308002	0.0
10	0.0	0.0	0.0	0.9054708250373074	0.0
11	0.0	0.0	0.0	1.087158741405449	0.0
12	0.0	0.0	0.0	1.2437111835047956	0.0
13	0.0	0.0	0.0	1.3151592639066465	0.0
14	0.0	0.0	0.0	1.3517739311485921	0.0
15	0.0	0.0	0.0	1.3915552723141658	0.0
16	0.0	0.0	0.0	1.48279506473869	0.0
17	0.0	0.0	0.0	1.5837327960543244	0.0
18	0.0	0.0	0.0	1.7470144202413798	0.0
19	0.0	0.0	0.0	1.81608749520051	0.0
20	0.0	0.0	0.0	1.8905043324057618	0.0
21	0.0	0.0	0.0	1.9890670582786751	0.0
22	0.0	0.0	0.0	2.0876297841515887	0.0
23	0.0	0.0	0.0	2.197473785877426	0.0
24	0.0	0.0	0.0	2.282973981815375	0.0
25	0.0	7.916684809069354E-4	0.0	2.353432476616092	0.0
26	0.0	7.916684809069354E-4	0.0	2.420328463252728	0.0
27	0.0	7.916684809069354E-4	0.0	2.508401581753625	0.0
28	0.0	7.916684809069354E-4	0.0	2.598255954336562	0.0
29	0.0	7.916684809069354E-4	0.0	2.689693663881313	0.0
30	0.0	7.916684809069354E-4	0.0	2.8082460188971266	0.0
31	0.0	7.916684809069354E-4	0.0	2.901662899644145	0.0
32	0.0	7.916684809069354E-4	0.0	3.0035902165609127	0.0
33	0.0	7.916684809069354E-4	0.0	3.1067050361990414	0.0
34	0.0	7.916684809069354E-4	0.0	3.21160110991921	0.0
35	0.0	7.916684809069354E-4	0.0	3.3424243263890814	0.0
36	0.0	7.916684809069354E-4	0.0	3.4451433117867563	0.0
37	0.0	7.916684809069354E-4	0.0	3.553206059430553	0.0
38	0.0	7.916684809069354E-4	0.0	3.676706342452035	0.0
39	0.0	7.916684809069354E-4	0.0	3.880956810526024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	25	3.8876908E-5	45.000004	20
ACCGGGT	20	7.0295297E-4	45.0	4
CGTTCGA	20	7.0295297E-4	45.0	14
TAGCCCG	35	1.2101191E-7	45.0	11
TGCGTAA	40	6.8012014E-9	45.0	1
CGTTTTA	805	0.0	44.16149	1
GTACGGG	85	0.0	42.35294	3
AGGCGAT	325	0.0	42.23077	7
TACGGGA	80	0.0	42.1875	4
ATTCGGC	55	6.002665E-11	40.909092	17
CCGCTCG	55	6.002665E-11	40.909092	19
CGTTTTT	1310	0.0	40.70611	1
TACGAAG	50	1.0786607E-9	40.500004	2
CGTAAAG	50	1.0786607E-9	40.500004	2
TACCTGG	315	0.0	40.0	10
CATATGC	310	0.0	39.919353	33
TATGGGC	85	0.0	39.705883	4
CGACGGT	125	0.0	39.6	28
CGGTCTA	125	0.0	39.6	31
ACGACGG	125	0.0	39.6	27
>>END_MODULE