FastQCFastQC Report
Thu 26 May 2016
SRR1545741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545741_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences334929
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10000.2985707418587223No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.2976750296331461No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.25557655503106624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT750.045.00000429
CGGGTAT253.8848662E-545.06
ATAGGCG207.026121E-445.05
CGACGCT207.026121E-445.012
TTTCGGC207.026121E-445.041
ATTCGGC207.026121E-445.017
GAATAAC351.208482E-745.09
GCTCTAC207.026121E-445.022
TCTACTC253.8848662E-545.042
CGCTCAT207.026121E-445.024
AGTGATA207.026121E-445.01
TGATTCG207.026121E-445.015
TTGCGTA207.026121E-445.01
GTACTAA207.026121E-445.01
TGTTACA207.026121E-445.032
TCCAATA207.026121E-445.037
CCGACGC207.026121E-445.011
ATGGTCG207.026121E-445.044
TGGATAG351.208482E-745.01
ACCGTAT253.8848662E-545.06