##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545741_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	334929
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.059358849188932	31.0	31.0	33.0	30.0	33.0
2	31.2218171612491	31.0	31.0	33.0	30.0	34.0
3	31.15497015785435	31.0	31.0	34.0	28.0	34.0
4	35.10961427645859	35.0	35.0	37.0	33.0	37.0
5	32.886796903224266	35.0	35.0	37.0	28.0	37.0
6	34.04727270555849	35.0	35.0	37.0	28.0	37.0
7	15.120649451077691	0.0	0.0	35.0	0.0	37.0
8	24.822807221829105	17.0	17.0	35.0	17.0	37.0
9	32.72919633713414	32.0	32.0	37.0	27.0	39.0
10	35.619859731465475	37.0	35.0	37.0	32.0	39.0
11	36.584947854619934	37.0	35.0	39.0	33.0	39.0
12	36.273672927695124	37.0	35.0	39.0	32.0	39.0
13	35.755258577190986	37.0	35.0	39.0	31.0	39.0
14	36.79515061401073	39.0	35.0	40.0	31.0	41.0
15	37.23765932481212	39.0	35.0	41.0	32.0	41.0
16	37.357660877379985	39.0	35.0	41.0	32.0	41.0
17	37.33910172006605	39.0	35.0	41.0	32.0	41.0
18	37.242967912602374	39.0	35.0	41.0	32.0	41.0
19	37.12952297352573	39.0	35.0	40.0	32.0	41.0
20	37.20603172612703	39.0	35.0	40.0	32.0	41.0
21	37.03898736747191	39.0	35.0	40.0	32.0	41.0
22	37.092318073382714	39.0	35.0	41.0	32.0	41.0
23	37.096542849380015	39.0	35.0	40.0	32.0	41.0
24	36.89350578779383	38.0	35.0	40.0	32.0	41.0
25	36.642127137393295	38.0	35.0	40.0	31.0	41.0
26	36.50522349512882	38.0	35.0	40.0	31.0	41.0
27	36.3744495101947	38.0	35.0	40.0	31.0	41.0
28	36.32468373894169	38.0	35.0	40.0	31.0	41.0
29	36.38285726228544	38.0	35.0	40.0	31.0	41.0
30	36.172108118437045	38.0	35.0	40.0	30.0	41.0
31	35.99843548931266	38.0	35.0	40.0	30.0	41.0
32	35.83481573706666	38.0	34.0	40.0	30.0	41.0
33	35.60201117251716	38.0	34.0	40.0	29.0	41.0
34	35.41670921299738	38.0	34.0	40.0	27.0	41.0
35	35.231592367337555	38.0	34.0	40.0	27.0	41.0
36	35.040074164972275	38.0	34.0	40.0	25.0	41.0
37	34.824589689158	38.0	34.0	40.0	25.0	41.0
38	34.78822078709219	37.0	34.0	40.0	25.0	41.0
39	34.71034159478576	37.0	34.0	40.0	24.0	41.0
40	34.49538857489199	37.0	33.0	40.0	23.0	41.0
41	34.42526625045905	37.0	33.0	40.0	23.0	41.0
42	34.385893726730146	37.0	33.0	40.0	23.0	41.0
43	34.29262918409573	37.0	33.0	40.0	23.0	41.0
44	34.06145481579678	36.0	33.0	40.0	23.0	41.0
45	33.96481642377937	36.0	33.0	40.0	23.0	41.0
46	33.981055686429066	36.0	33.0	40.0	23.0	41.0
47	33.97131332312222	36.0	33.0	40.0	23.0	41.0
48	33.94957438740748	36.0	33.0	40.0	23.0	41.0
49	33.86873635904923	36.0	33.0	40.0	23.0	41.0
50	33.70714987355529	36.0	33.0	40.0	23.0	41.0
51	33.50275132938623	35.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	11.0
16	41.0
17	95.0
18	253.0
19	395.0
20	694.0
21	1206.0
22	1800.0
23	2724.0
24	3777.0
25	5128.0
26	6140.0
27	6667.0
28	7000.0
29	7999.0
30	9782.0
31	12721.0
32	17187.0
33	26912.0
34	34527.0
35	34298.0
36	36926.0
37	56501.0
38	52842.0
39	9296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.785330622310997	24.88467705095707	28.895079255603427	15.43491307112851
2	32.889358640189414	24.33291832000215	30.319261694269535	12.458461345538906
3	30.21744907129571	22.775274759725196	33.73162670297287	13.275649466006229
4	27.04901635869095	26.983629366223887	30.762639245929734	15.204715029155432
5	20.69453525971176	36.82601387159667	29.165285776985574	13.314165091706004
6	20.756040832534655	40.54202532477033	28.951509125814724	9.750424716880294
7	37.07561901179056	57.55369048365474	4.703683467242311	0.6670070373123855
8	87.48391450128236	3.5201490465143355	7.550854061607088	1.445082390596216
9	83.14000877797982	4.883423053841262	9.309137160413103	2.667431007765825
10	57.512189150536386	19.35514691173353	13.681705674934092	9.450958262795996
11	53.05452797458566	17.564319602064916	18.053079906487643	11.328072516861782
12	46.597637111148934	20.103962332315206	22.13484051843824	11.163560038097627
13	21.039981608042304	44.47957626840315	22.560005254845056	11.920436868709487
14	13.59332873534391	46.466265984731095	27.11798620006031	12.822419079864689
15	12.655219464423803	21.381247965986823	53.22023473631725	12.743297833272127
16	13.683795670127102	15.100812410988599	52.33168820854569	18.883703710338608
17	14.34542843408603	16.190297048031074	29.519689247571872	39.94458527031102
18	20.96593606406134	21.936589545844047	35.968817271720276	21.128657118374342
19	30.161914913309985	22.882163085310616	26.273329571341986	20.68259243003741
20	32.59198218129812	20.90502763272216	24.99843250360524	21.50455768237447
21	21.466340627416557	27.336241412359037	29.65106037398971	21.546357586234695
22	22.79289043349486	23.379880511989107	25.242961941187538	28.584267113328497
23	19.65491193655969	29.63852040283165	24.528183585177754	26.17838407543091
24	19.473082354767726	22.103192019801213	41.94053067963658	16.483194945794484
25	17.035849388974977	22.967554317482215	38.74821230768312	21.24838398585969
26	15.925763370744248	34.24606409119545	28.244792179835127	21.583380358225178
27	17.306951622582698	34.23591268597225	29.58627052300637	18.870865168438684
28	14.477098131245727	28.38721042370174	41.14304822813193	15.992643216920602
29	14.03521343329482	24.326946905164977	40.242558870686025	21.395280790854184
30	18.138471138659238	29.554024882885628	33.080443914979	19.227060063476138
31	30.769207802250627	25.608113958480754	24.832427171131794	18.79025106813683
32	31.469953333393047	24.976935410191416	27.313251465235915	16.239859791179622
33	29.204099973427205	25.459425729035107	25.364480233124038	19.97199406441365
34	19.42172818716803	27.751254743542663	28.581579976651767	24.245437092637545
35	20.296540460814082	24.913936983659223	31.937515115143807	22.852007440382888
36	33.62921693851533	22.695556371648916	24.862284245317664	18.81294244451809
37	20.55241558658701	33.08551961759059	28.123871029382347	18.23819376644005
38	21.363632292217154	33.38558321315861	23.162819582657818	22.087964911966417
39	21.731471446187104	30.82384624801077	26.252429619411878	21.19225268639025
40	24.471455144224596	25.107410824383674	25.55765550310663	24.8634785282851
41	17.365770058728867	23.752198227086936	27.730354791612548	31.151676922571646
42	23.329123485873126	24.99395394247736	23.828333766260908	27.8485888053886
43	22.750194817409064	25.791734964723865	25.044710968593343	26.413359249273725
44	19.94004699503477	30.19296627046329	28.35705477877401	21.509931955727932
45	16.933439624517437	38.47024294701265	22.275467337853694	22.320850090616222
46	22.048255003299207	32.52898375476593	26.56085319575194	18.861908046182922
47	23.798476692075038	26.951085155361287	26.093888555484895	23.156549597078786
48	23.990756249832053	23.030552744014404	30.42734430282239	22.551346703331156
49	21.675340146717662	23.547080127429993	32.82665878439908	21.950920941453266
50	20.01857110014361	33.810150808081715	25.766953593149594	20.40432449862508
51	19.540559342427798	34.12096295035664	23.5918657387088	22.74661196850676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	247.0
1	319.0
2	391.0
3	1897.5
4	3404.0
5	2608.0
6	1812.0
7	1884.5
8	1957.0
9	2077.0
10	2197.0
11	2183.5
12	2170.0
13	2067.0
14	1964.0
15	1822.5
16	1681.0
17	1570.5
18	1460.0
19	1437.5
20	1415.0
21	1399.0
22	1383.0
23	1372.0
24	1361.0
25	1726.0
26	2294.5
27	2498.0
28	3096.0
29	3694.0
30	4291.0
31	4888.0
32	5549.0
33	6210.0
34	6388.5
35	6567.0
36	7802.5
37	9038.0
38	13283.5
39	17529.0
40	23658.5
41	29788.0
42	32608.5
43	35429.0
44	34071.5
45	32714.0
46	31289.5
47	29865.0
48	27966.0
49	26067.0
50	24349.0
51	22631.0
52	20176.5
53	17722.0
54	16022.0
55	14322.0
56	13174.0
57	12026.0
58	11384.5
59	10743.0
60	9870.5
61	8998.0
62	7706.0
63	6414.0
64	5511.0
65	4608.0
66	3948.5
67	3289.0
68	2853.5
69	2418.0
70	1985.5
71	1553.0
72	1318.5
73	1084.0
74	838.0
75	462.0
76	332.0
77	248.0
78	164.0
79	134.0
80	104.0
81	82.5
82	61.0
83	45.5
84	30.0
85	20.5
86	11.0
87	6.0
88	1.0
89	3.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.802522325627223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.09234150184517	19.900337086367557
2	7.1422647924700415	4.114304822813193
3	3.909068292076129	3.3777308026477257
4	2.729402496164531	3.144547053256063
5	2.054567317659742	2.958836051819938
6	1.6658373761247254	2.8788190930018005
7	1.2822905004768421	2.585324053754676
8	1.1568603060082099	2.6656395833146727
9	0.9837459053779491	2.5500927062153473
>10	9.844715346021479	51.96504333754318
>50	0.11402744951693826	2.1344822335480056
>100	0.02176887672596094	0.8730208491949041
>500	0.003109839532280134	0.8518223265229348
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1000	0.2985707418587223	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.2976750296331461	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.25557655503106624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007165697804609335	0.0
2	0.0	0.0	0.0	0.032245640120742006	0.0
3	0.0	0.0	0.0	0.03941133792535134	0.0
4	0.0	0.0	0.0	0.059714148371744456	0.0
5	0.0	0.0	0.0	0.10778403781099875	0.0
6	0.0	0.0	0.0	0.15256964908980708	0.0
7	0.0	0.0	0.0	0.1797395865989508	0.0
8	0.0	0.0	0.0	0.3669434417443697	0.0
9	0.0	0.0	0.0	0.46815892323447655	0.0
10	0.0	0.0	0.0	0.6434199487055465	0.0
11	0.0	0.0	0.0	0.7944967440860601	0.0
12	0.0	0.0	0.0	0.9324364268247898	0.0
13	0.0	0.0	0.0	0.9888662970360882	0.0
14	0.0	0.0	0.0	1.0094676782243401	0.0
15	0.0	0.0	0.0	1.046191879472963	0.0
16	0.0	0.0	0.0	1.1172517160353388	0.0
17	0.0	0.0	0.0	1.2080172215603904	0.0
18	0.0	0.0	0.0	1.3489426117177072	0.0
19	0.0	0.0	0.0	1.3985053548662552	0.0
20	0.0	0.0	0.0	1.4573237910124235	0.0
21	0.0	0.0	0.0	1.5361464668631262	0.0
22	0.0	0.0	0.0	1.6072063034255022	0.0
23	0.0	0.0	0.0	1.6884175452110746	0.0
24	0.0	0.0	0.0	1.7499231180339714	0.0
25	0.0	0.0	0.0	1.7908273096686163	0.0
26	0.0	0.0	0.0	1.842778618752034	0.0
27	0.0	0.0	0.0	1.894431357093593	0.0
28	0.0	0.0	0.0	1.9457855246932931	0.0
29	0.0	0.0	0.0	1.997736833776711	0.0
30	0.0	0.0	0.0	2.0744695144344023	0.0
31	0.0	0.0	0.0	2.148813629157224	0.0
32	0.0	0.0	0.0	2.216887758301013	0.0
33	0.0	0.0	0.0	2.2795876140913447	0.0
34	0.0	0.0	0.0	2.3401974746886656	0.0
35	0.0	0.0	0.0	2.4375315365346086	0.0
36	0.0	0.0	0.0	2.5020228167760927	0.0
37	0.0	0.0	0.0	2.5733812240803275	0.0
38	0.0	0.0	0.0	2.640261070256681	0.0
39	0.0	0.0	0.0	2.7152023264632206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGCAT	75	0.0	45.000004	29
CGGGTAT	25	3.8848662E-5	45.0	6
ATAGGCG	20	7.026121E-4	45.0	5
CGACGCT	20	7.026121E-4	45.0	12
TTTCGGC	20	7.026121E-4	45.0	41
ATTCGGC	20	7.026121E-4	45.0	17
GAATAAC	35	1.208482E-7	45.0	9
GCTCTAC	20	7.026121E-4	45.0	22
TCTACTC	25	3.8848662E-5	45.0	42
CGCTCAT	20	7.026121E-4	45.0	24
AGTGATA	20	7.026121E-4	45.0	1
TGATTCG	20	7.026121E-4	45.0	15
TTGCGTA	20	7.026121E-4	45.0	1
GTACTAA	20	7.026121E-4	45.0	1
TGTTACA	20	7.026121E-4	45.0	32
TCCAATA	20	7.026121E-4	45.0	37
CCGACGC	20	7.026121E-4	45.0	11
ATGGTCG	20	7.026121E-4	45.0	44
TGGATAG	35	1.208482E-7	45.0	1
ACCGTAT	25	3.8848662E-5	45.0	6
>>END_MODULE