FastQCFastQC Report
Thu 26 May 2016
SRR1545740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545740_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences464302
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.4820138616676215No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.2022390599222058No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.188239550981904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8240.17747069795090264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG408.294592E-944.0000041
CGCATAG207.8516273E-444.0000042
TAGCGCA207.8516273E-444.0000041
CCAATCG207.8516273E-444.00000418
ATCCGCA207.8516273E-444.0000041
ACGATAA207.8516273E-444.0000041
CGTAGGA207.8516273E-444.0000044
AGCGCAG302.5244444E-644.0000042
ATGACGG207.8516273E-444.0000042
GCGATAA207.8516273E-444.0000049
CGTGTGA207.8516273E-444.00000419
CGTAAAC207.8516273E-444.00000423
CCGCCCC207.8516273E-444.00000411
GCGAATG254.4387285E-544.01
GCGAATA254.4387285E-544.01
GCGCGCT254.4387285E-544.029
GCGCGAC1150.044.09
CTATACG254.4387285E-544.017
CGTTAAG454.783942E-1044.02
TGTTACG254.4387285E-544.01
Produced by FastQC (version 0.11.5)