##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545740_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	464302
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.631769839457938	31.0	31.0	33.0	30.0	34.0
2	31.810537107313774	31.0	31.0	34.0	30.0	34.0
3	31.80423086697882	31.0	31.0	34.0	30.0	34.0
4	35.694567329022924	37.0	35.0	37.0	35.0	37.0
5	27.1860147059457	35.0	26.0	37.0	0.0	37.0
6	31.409414562073824	36.0	28.0	37.0	17.0	37.0
7	14.986297711403354	0.0	0.0	32.0	0.0	37.0
8	25.143010368251698	17.0	17.0	35.0	17.0	37.0
9	33.423743597916875	32.0	32.0	37.0	28.0	39.0
10	36.24572368846139	37.0	35.0	39.0	33.0	39.0
11	37.11202407054029	39.0	37.0	39.0	34.0	39.0
12	37.03916201093254	39.0	35.0	39.0	34.0	39.0
13	36.553090445442834	39.0	35.0	39.0	33.0	39.0
14	37.89673100697391	40.0	36.0	41.0	33.0	41.0
15	38.19236832923399	40.0	36.0	41.0	33.0	41.0
16	38.24824575384125	40.0	36.0	41.0	34.0	41.0
17	38.27076773307029	40.0	36.0	41.0	34.0	41.0
18	38.2363827853423	40.0	36.0	41.0	34.0	41.0
19	38.17396435940401	40.0	36.0	41.0	34.0	41.0
20	38.168797463719734	40.0	36.0	41.0	34.0	41.0
21	38.01725385632627	40.0	36.0	41.0	34.0	41.0
22	37.89963859729228	40.0	35.0	41.0	34.0	41.0
23	37.766789718760634	39.0	35.0	41.0	34.0	41.0
24	37.621933569099426	39.0	35.0	41.0	34.0	41.0
25	37.461688728456906	39.0	35.0	41.0	33.0	41.0
26	37.224674888326994	39.0	35.0	40.0	33.0	41.0
27	36.99892957600872	39.0	35.0	40.0	33.0	41.0
28	37.052052328010646	39.0	35.0	40.0	33.0	41.0
29	37.03878940861767	39.0	35.0	40.0	33.0	41.0
30	36.794088761194224	38.0	35.0	40.0	32.0	41.0
31	36.529808185189815	38.0	35.0	40.0	32.0	41.0
32	36.19699893603732	38.0	35.0	40.0	31.0	41.0
33	35.911529564809115	38.0	35.0	40.0	30.0	41.0
34	35.623008300631916	38.0	35.0	40.0	29.0	41.0
35	35.41705829395523	38.0	35.0	40.0	27.0	41.0
36	35.216154571808865	38.0	35.0	40.0	26.0	41.0
37	35.027826716232106	38.0	34.0	40.0	25.0	41.0
38	34.8937049592722	38.0	34.0	40.0	25.0	41.0
39	34.8550081627906	38.0	34.0	40.0	25.0	41.0
40	34.61810201119099	38.0	34.0	40.0	24.0	41.0
41	34.47909765626682	38.0	34.0	40.0	23.0	40.0
42	34.48247476857735	38.0	34.0	40.0	23.0	41.0
43	34.360019986991226	37.0	34.0	40.0	23.0	41.0
44	34.13371900185655	37.0	34.0	40.0	23.0	40.0
45	33.88321825019061	37.0	33.0	40.0	23.0	40.0
46	33.86137255493192	37.0	33.0	40.0	23.0	40.0
47	33.724715809968515	36.0	33.0	39.0	23.0	40.0
48	33.70666075097674	36.0	33.0	39.0	23.0	40.0
49	33.63337439855956	36.0	33.0	39.0	22.0	40.0
50	33.67576491163079	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	2.0
15	8.0
16	21.0
17	41.0
18	151.0
19	348.0
20	611.0
21	1124.0
22	1935.0
23	3083.0
24	4991.0
25	7229.0
26	9093.0
27	9067.0
28	8435.0
29	8905.0
30	10410.0
31	13821.0
32	20008.0
33	39031.0
34	38332.0
35	46256.0
36	58388.0
37	116901.0
38	53025.0
39	13083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.606157199409008	23.050514535797824	28.818958350384015	17.524369914409156
2	34.05046715284448	23.91805333597529	30.145034912621526	11.886444598558697
3	27.80690154253051	22.758893995718303	37.10300623301214	12.331198228739053
4	24.727224952724736	26.602513019543316	34.747642698071516	13.922619329660439
5	16.721659609478316	47.20892867142506	25.938720918712388	10.130690800384233
6	19.02877868284005	38.70799608875258	31.97767832143734	10.285546906970032
7	38.436836369431965	55.71373804118871	5.136312141666415	0.713113447712911
8	87.37739660824205	2.0204522056764778	9.730520221752222	0.8716309643292511
9	83.82647500979967	3.8698950252206537	10.645226598205479	1.6584033667742117
10	51.49923971897601	26.363444482255083	14.964613548940129	7.172702249828775
11	43.24491387071346	19.330306567708085	26.3496603503754	11.075119211203054
12	39.979366877592604	21.046861740849707	26.896502707289656	12.07726867426804
13	19.4293800155933	41.192370483004595	27.324672303802267	12.053577197599838
14	14.206270918497014	42.81825191362518	29.900581948817795	13.074895219060009
15	13.001667018449197	22.96285607212547	51.52357732682608	12.511899582599256
16	14.592441988188723	18.8207244422811	49.72044057531521	16.86639299421497
17	15.788646182872354	18.241144772152605	32.36492627643215	33.605282768542885
18	20.13904743033629	22.855382918876078	38.09503297422798	18.91053667655965
19	27.20427652691567	23.22001628250578	30.13792746962107	19.43777972095748
20	29.579023997312092	22.94325675960905	28.668625162071237	18.809094081007622
21	20.977510327330055	27.34750227222799	32.29902089588242	19.375966504559532
22	21.879724834267353	22.86938242781638	30.23204724511202	25.018845492804253
23	17.32816141218431	29.56653212779613	29.293434014929936	23.81187244508962
24	18.037828826927303	23.551266201739384	42.84065112792967	15.570253843403648
25	15.04904135670318	24.644089407325403	40.765277771795084	19.541591464176335
26	15.235988645321363	34.321411495104485	31.409944389642945	19.03265546993121
27	16.197431843929166	33.145883498240366	32.913491649831364	17.743193007999103
28	14.088028912216618	27.49180490284341	42.49389406033142	15.92627212460855
29	14.40894073254046	23.25619962868995	41.78336513734595	20.551494501423644
30	17.33613036342725	29.54800970058281	35.49758562315045	17.61827431283949
31	27.060620027482113	26.068808663326887	28.867418189023525	18.00315312016748
32	28.179934611524395	26.759307519674696	29.468104811092783	15.59265305770813
33	27.022282910691747	27.082373110604735	28.384758196174044	17.510585782529475
34	18.454368062166434	27.867637873625355	30.912854133731926	22.765139930476288
35	19.21529521733699	25.735620350547705	33.58116053775345	21.46792389436186
36	28.7037316229523	24.276010010725777	29.097440889765714	17.922817476556208
37	19.343229191345287	31.324439696576796	31.317763007697575	18.014568104380338
38	19.90299417189674	32.10173550835448	27.31024204074072	20.68502827900806
39	19.89782512244186	31.661504796447137	29.193929812923486	19.246740268187516
40	22.918273020577125	26.713432205762626	27.053512584481652	23.314782189178594
41	17.349052987064454	25.09293520165754	29.197591222954028	28.360420588323983
42	22.10156320670598	26.162066930575357	25.985242363806314	25.751127498912346
43	22.326847612114527	27.279012366950823	26.96714638317302	23.42699363776163
44	19.576267170936156	31.937833565222633	28.865264418417325	19.620634845423883
45	17.145090910657288	37.63778747453166	24.327269751153345	20.889851863657704
46	21.826526700294206	31.986939535044	27.574509694121495	18.612024070540294
47	21.24931617783253	28.254670451559544	28.106275656792345	22.389737713815578
48	22.89673531451512	25.215484749150335	31.385606781792884	20.502173154541655
49	20.873698584111203	24.429358477887238	32.4342776899518	22.26266524804976
50	19.390827521742317	32.84629400691791	27.930743352387022	19.832135118952753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	439.5
2	585.0
3	3602.0
4	6619.0
5	5102.0
6	3585.0
7	3710.0
8	3835.0
9	4146.5
10	4458.0
11	4529.0
12	4600.0
13	4411.5
14	4223.0
15	3883.5
16	3544.0
17	3375.5
18	3207.0
19	3026.5
20	2846.0
21	2801.0
22	2756.0
23	2882.5
24	3009.0
25	3675.0
26	4341.0
27	4706.0
28	5071.0
29	6391.0
30	7711.0
31	8989.5
32	10268.0
33	11482.5
34	12697.0
35	13766.5
36	14836.0
37	17373.0
38	19910.0
39	26764.0
40	33618.0
41	40788.0
42	47958.0
43	49115.0
44	50272.0
45	47971.5
46	45671.0
47	42304.5
48	38938.0
49	36445.0
50	33952.0
51	30709.0
52	27466.0
53	23462.0
54	19458.0
55	16942.5
56	14427.0
57	12770.5
58	11114.0
59	9627.0
60	8140.0
61	6985.0
62	5830.0
63	4636.5
64	3443.0
65	2664.5
66	1886.0
67	1601.5
68	1317.0
69	1141.0
70	965.0
71	810.0
72	655.0
73	520.0
74	385.0
75	279.5
76	174.0
77	152.5
78	131.0
79	95.0
80	59.0
81	42.0
82	25.0
83	18.5
84	12.0
85	9.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	464302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.697140366197566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.78551120257225	22.16392374584962
2	8.694643348423476	5.685799479948567
3	4.7674943822771825	4.676502990371262
4	3.228063699307962	4.221938075491976
5	2.356623495651675	3.8527424613800996
6	1.9020681370037675	3.7315313317010457
7	1.5243694212631542	3.4889764655886486
8	1.2845949929592468	3.360206623880245
9	1.036685026526205	3.0506972245076316
>10	7.332093323948173	42.90199061262132
>50	0.08244663344749235	1.6656317890751837
>100	0.0027031683097538473	0.12285636438389522
>500	0.0020273762323153857	0.5826838720869275
>1k	6.757920774384618E-4	0.49451896311359256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2238	0.4820138616676215	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	939	0.2022390599222058	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	874	0.188239550981904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	824	0.17747069795090264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00689206593984088	0.0
2	0.0	0.0	0.0	0.02735288669874349	0.0
3	0.0	0.0	0.0	0.036398723244784645	0.0
4	0.0	0.0	0.0	0.04867521570012621	0.0
5	0.0	0.0	0.0	0.07258206942894926	0.0
6	0.0	0.0	0.0	0.11178069446179427	0.0
7	0.0	0.0	0.0	0.13827207291805765	0.0
8	0.0	0.0	0.0	0.3092814590503595	0.0
9	0.0	0.0	0.0	0.3728176919332676	0.0
10	0.0	0.0	0.0	0.501613174184044	0.0
11	0.0	0.0	0.0	0.6762839703468863	0.0
12	0.0	0.0	0.0	0.8098177479313033	0.0
13	0.0	0.0	0.0	0.8744308661173116	0.0
14	0.0	0.0	0.0	0.8994146051492348	0.0
15	0.0	0.0	0.0	0.9312904101209988	0.0
16	0.0	0.0	0.0	1.0135644472778493	0.0
17	0.0	0.0	0.0	1.121683731709103	0.0
18	0.0	0.0	0.0	1.2756783300524228	0.0
19	0.0	0.0	0.0	1.348260399481372	0.0
20	0.0	0.0	0.0	1.418042567122261	0.0
21	0.0	0.0	0.0	1.511946965552593	0.0
22	0.0	0.0	0.0	1.6112357904984256	0.0
23	0.0	0.0	0.0	1.7413235351129221	0.0
24	0.0	0.0	0.0	1.8403969829981348	0.0
25	0.0	0.0	0.0	1.9086715112146835	0.0
26	0.0	0.0	0.0	1.9874995154016135	0.0
27	0.0	0.0	0.0	2.063958371921723	0.0
28	0.0	0.0	0.0	2.149463064987874	0.0
29	0.0	0.0	0.0	2.2293679544779046	0.0
30	0.0	0.0	0.0	2.343948550727759	0.0
31	0.0	0.0	0.0	2.4563753763714136	0.0
32	0.0	0.0	0.0	2.546187610649965	0.0
33	0.0	0.0	0.0	2.6362152219891364	0.0
34	0.0	0.0	0.0	2.7406730963898496	0.0
35	0.0	0.0	0.0	2.871191595125586	0.0
36	0.0	0.0	0.0	2.9922335031940417	0.0
37	0.0	0.0	0.0	3.102075804110256	0.0
38	0.0	0.0	0.0	3.2078259408746894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	40	8.294592E-9	44.000004	1
CGCATAG	20	7.8516273E-4	44.000004	2
TAGCGCA	20	7.8516273E-4	44.000004	1
CCAATCG	20	7.8516273E-4	44.000004	18
ATCCGCA	20	7.8516273E-4	44.000004	1
ACGATAA	20	7.8516273E-4	44.000004	1
CGTAGGA	20	7.8516273E-4	44.000004	4
AGCGCAG	30	2.5244444E-6	44.000004	2
ATGACGG	20	7.8516273E-4	44.000004	2
GCGATAA	20	7.8516273E-4	44.000004	9
CGTGTGA	20	7.8516273E-4	44.000004	19
CGTAAAC	20	7.8516273E-4	44.000004	23
CCGCCCC	20	7.8516273E-4	44.000004	11
GCGAATG	25	4.4387285E-5	44.0	1
GCGAATA	25	4.4387285E-5	44.0	1
GCGCGCT	25	4.4387285E-5	44.0	29
GCGCGAC	115	0.0	44.0	9
CTATACG	25	4.4387285E-5	44.0	17
CGTTAAG	45	4.783942E-10	44.0	2
TGTTACG	25	4.4387285E-5	44.0	1
>>END_MODULE