FastQCFastQC Report
Thu 26 May 2016
SRR1545739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545739_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences180484
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.36734558187983424No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.301411759491146No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3030.16788191751069345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT950.045.0000047
TCACGGG207.0174714E-445.03
GGTACTC207.0174714E-445.09
GCGACCT351.2043347E-745.011
CACGGGG453.8016879E-1045.04
GCGCGAG207.0174714E-445.02
GCGCGAC351.2043347E-745.09
CGGTTTA253.8777012E-545.01
GACGTGG351.2043347E-745.025
ATTACGA207.0174714E-445.01
TGGCGAC351.2043347E-745.029
CGACCTC351.2043347E-745.012
ATGCTAG253.8777012E-545.02
TACGGGA207.0174714E-445.04
CGCGACC351.2043347E-745.010
GTGGCGA351.2043347E-745.028
CGTTAAG207.0174714E-445.02
AAACGTA253.8777012E-545.024
GTTACGG207.0174714E-445.02
AGCGGGA253.8777012E-545.04
Produced by FastQC (version 0.11.5)