##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545739_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	180484
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119999556747413	31.0	31.0	33.0	30.0	34.0
2	31.25599499124576	31.0	31.0	33.0	30.0	34.0
3	31.14101527005164	31.0	31.0	34.0	28.0	34.0
4	35.096429600407795	35.0	35.0	37.0	32.0	37.0
5	32.91132731987323	35.0	35.0	37.0	28.0	37.0
6	34.09480064714878	35.0	35.0	37.0	29.0	37.0
7	15.349271957625053	0.0	0.0	35.0	0.0	37.0
8	24.99803860730037	17.0	17.0	35.0	17.0	37.0
9	32.95066044635536	32.0	32.0	37.0	28.0	39.0
10	35.732353006360675	37.0	35.0	38.0	32.0	39.0
11	36.56039870570245	37.0	35.0	39.0	32.0	39.0
12	36.38554664125352	37.0	35.0	39.0	32.0	39.0
13	36.03318853748809	37.0	35.0	39.0	32.0	39.0
14	37.10236918508012	39.0	35.0	40.0	32.0	41.0
15	37.50548525077015	39.0	36.0	41.0	32.0	41.0
16	37.588838899847076	39.0	35.0	41.0	33.0	41.0
17	37.53985394827242	39.0	35.0	41.0	33.0	41.0
18	37.44480951220053	39.0	36.0	41.0	32.0	41.0
19	37.346102701624524	39.0	36.0	41.0	32.0	41.0
20	37.4156711952306	39.0	35.0	41.0	32.0	41.0
21	37.26096496088296	39.0	35.0	41.0	32.0	41.0
22	37.28606413864941	39.0	35.0	41.0	32.0	41.0
23	37.263208927107115	39.0	35.0	41.0	33.0	41.0
24	37.092074643735735	39.0	35.0	41.0	32.0	41.0
25	36.85567695751424	38.0	35.0	40.0	32.0	41.0
26	36.74742359433523	38.0	35.0	40.0	32.0	41.0
27	36.66030783892201	38.0	35.0	40.0	31.0	41.0
28	36.61202655082999	38.0	35.0	40.0	31.0	41.0
29	36.662906407216155	38.0	35.0	40.0	31.0	41.0
30	36.38336916291749	38.0	35.0	40.0	31.0	41.0
31	36.191667959486715	38.0	35.0	40.0	30.0	41.0
32	35.99447596462844	38.0	35.0	40.0	30.0	41.0
33	35.7129330023714	38.0	35.0	40.0	29.0	41.0
34	35.51349150063164	38.0	34.0	40.0	27.0	41.0
35	35.288291482901535	38.0	34.0	40.0	26.0	41.0
36	35.084112719132996	38.0	34.0	40.0	25.0	41.0
37	34.922037410518385	38.0	34.0	40.0	24.0	41.0
38	34.85969393408834	38.0	34.0	40.0	24.0	41.0
39	34.79227521553157	38.0	34.0	40.0	24.0	41.0
40	34.640771481128525	38.0	34.0	40.0	23.0	41.0
41	34.54214778041267	38.0	34.0	40.0	23.0	41.0
42	34.50227721016822	38.0	34.0	40.0	23.0	41.0
43	34.40330444803971	38.0	33.0	40.0	23.0	41.0
44	34.16092285188715	37.0	33.0	40.0	22.0	41.0
45	34.095775802841246	37.0	33.0	40.0	22.0	41.0
46	34.104108951486005	37.0	33.0	40.0	22.0	41.0
47	34.058021763702044	37.0	33.0	40.0	22.0	41.0
48	34.050674852064446	37.0	33.0	40.0	22.0	41.0
49	33.99210456328539	37.0	33.0	40.0	22.0	41.0
50	33.830527913831695	37.0	33.0	40.0	20.0	41.0
51	33.68025974601627	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	19.0
17	52.0
18	110.0
19	202.0
20	314.0
21	624.0
22	899.0
23	1520.0
24	2286.0
25	3089.0
26	3630.0
27	3497.0
28	3457.0
29	3863.0
30	4737.0
31	6223.0
32	8477.0
33	13157.0
34	17092.0
35	17617.0
36	20249.0
37	32341.0
38	30853.0
39	6169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.38718113516988	23.69129673544469	29.108397420269945	16.813124709115492
2	33.42235322798697	23.868597770439486	30.616564349194388	12.092484652379158
3	28.32273220894927	22.667383258349773	36.68303007468806	12.326854458012898
4	25.190598612619404	27.09935506748521	33.58192415948228	14.128122160413112
5	20.379645841182597	35.716739433966445	31.395580771702754	12.508033953148201
6	18.921898894084794	39.388533055561716	31.495312603887328	10.194255446466169
7	37.37727443983954	56.61332860530573	5.50741339952572	0.5019835553290043
8	88.41725582323086	2.0694355178298354	8.713237738525299	0.800070920413998
9	84.6274462002172	4.001462733538707	9.623013674342323	1.7480773919017751
10	51.965271159770396	26.95474391081758	14.087675361804925	6.9923095676071005
11	43.575053744376234	19.80618780612132	25.779570488242726	10.839187961259723
12	40.1404002570865	21.32986857560781	26.25274262538508	12.276988541920614
13	19.707010039671108	41.819219432193435	26.39901597925578	12.07475454887968
14	14.269962988408944	43.57671594157931	29.00312493074178	13.150196139269962
15	12.967908512665943	23.49183307107555	51.117550586201546	12.422707830056957
16	14.860042995500985	19.11028124376676	49.204361605460875	16.825314155271382
17	15.926619534141532	18.5539992464706	31.764588550785668	33.7547926686022
18	20.247224130670862	23.05910773254139	37.69974069723632	18.993927439551427
19	27.714922098357746	23.406506947984308	29.58378582034973	19.294785133308213
20	30.222069546330978	23.179339996897234	27.930453669023294	18.668136787748498
21	21.219055428736063	27.938210589304312	31.530218745151924	19.312515236807695
22	22.049599964539794	23.195407903193637	29.392079076261606	25.362913056004967
23	17.53451829525055	29.856940227388577	28.669023292923473	23.939518184437404
24	17.932337492520112	23.718445956428273	42.70461647569868	15.64460007535294
25	15.171427938210588	24.9556747412513	40.65291106136832	19.21998625916979
26	15.194144633319299	34.96764256111345	30.83154185412557	19.006670951441677
27	16.025797300591744	34.39640078898961	32.02167505152811	17.55612685889054
28	14.263868265330998	28.1692560005319	41.66075663216684	15.906119101970258
29	14.373573280734025	23.663593448726758	41.301167970568024	20.661665299971187
30	17.456395026705966	30.587752931007735	34.65958201281	17.296270029476297
31	27.716584295560825	26.497085614237275	28.132133596329865	17.654196493872032
32	28.995922076195118	26.53864054431418	29.00312493074178	15.46231244874892
33	27.7625717515126	27.07608430664214	27.647326078765982	17.514017863079275
34	18.908047250725826	27.803018550120782	29.936725693136236	23.352208506017156
35	19.120808492719576	25.8161388267104	33.60741118326278	21.45564149730724
36	29.341659094434963	24.585558830699675	28.523304004787125	17.549478070078234
37	19.464329248021986	32.08262228230757	30.97061235344961	17.48243611622083
38	19.73637552359212	32.869395625096956	27.005717958378582	20.388510892932338
39	19.894284257884358	32.38347997606436	28.91336628177567	18.808869484275615
40	23.340019059861262	26.82121406883713	26.64502116531105	23.19374570599056
41	17.33837902528756	25.196693335697347	28.852973116730567	28.61195452228452
42	21.92050264843421	25.81503069524168	25.658230092418165	26.606236563905945
43	22.332727554797103	26.78963232197868	26.987433789144745	23.890206334079476
44	19.176769131889806	31.85767159415793	29.374903038496488	19.590656235455775
45	17.048048580483588	38.19618359522174	24.20491567119523	20.550852153099445
46	21.752066665189158	32.602335941136054	27.153099443718006	18.492497949956782
47	20.811263048248044	28.568183329270187	27.734868464794665	22.885685157687107
48	22.36430930165555	25.67263580151149	31.70197912280313	20.26107577402983
49	20.949779481837723	25.00997318321846	32.51700981804481	21.523237516899005
50	19.66656324106292	33.523747257374616	27.307683783604087	19.50200571795838
51	18.55510737793932	34.254559960993774	25.832760798741162	21.35757186232575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	164.0
2	197.0
3	1191.5
4	2186.0
5	1678.0
6	1170.0
7	1243.0
8	1316.0
9	1386.0
10	1456.0
11	1501.0
12	1546.0
13	1473.0
14	1400.0
15	1334.5
16	1269.0
17	1208.5
18	1148.0
19	1073.0
20	998.0
21	993.5
22	989.0
23	1063.0
24	1137.0
25	1310.0
26	1673.5
27	1864.0
28	2273.0
29	2682.0
30	3196.5
31	3711.0
32	4357.0
33	5003.0
34	5443.0
35	5883.0
36	6419.5
37	6956.0
38	9278.0
39	11600.0
40	14203.0
41	16806.0
42	18086.0
43	19366.0
44	18451.0
45	17536.0
46	16697.5
47	15859.0
48	14819.0
49	13779.0
50	12624.5
51	11470.0
52	10172.0
53	8874.0
54	7811.5
55	6749.0
56	5911.0
57	5073.0
58	4403.5
59	3734.0
60	3177.0
61	2620.0
62	2159.0
63	1698.0
64	1313.5
65	929.0
66	757.5
67	586.0
68	502.0
69	418.0
70	380.0
71	342.0
72	282.5
73	223.0
74	178.5
75	106.0
76	78.0
77	61.5
78	45.0
79	35.5
80	26.0
81	18.0
82	10.0
83	6.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	180484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.8587354003679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.973647641426	29.37379490702777
2	11.182697895348543	9.809179761086856
3	5.837691705197201	7.681013275414996
4	3.8757927183607466	6.799494692050264
5	2.8297834710326186	6.205536224817712
6	1.9871649106849594	5.22927240087764
7	1.633441976805882	5.014848961680814
8	1.2115010485358397	4.250792314000133
9	0.9487354405113823	3.744930298530617
>10	3.5119634149422674	20.91321114337005
>50	0.0025265923848505523	0.08310986015380864
>100	0.0025265923848505523	0.22605881961835955
>500	0.0025265923848505523	0.6687573413709802
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	663	0.36734558187983424	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	544	0.301411759491146	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	303	0.16788191751069345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004986591609228519	0.0
2	0.0	0.0	0.0	0.025487023780501317	0.0
3	0.0	0.0	0.0	0.02991954965537111	0.0
4	0.0	0.0	0.0	0.038784601405110704	0.0
5	0.0	0.0	0.0	0.06316349371689457	0.0
6	0.0	0.0	0.0	0.08865051749739589	0.0
7	0.0	0.0	0.0	0.10194809512200527	0.0
8	0.0	0.0	0.0	0.22384255668092462	0.0
9	0.0	0.0	0.0	0.2764788014450034	0.0
10	0.0	0.0	0.0	0.3800890937700849	0.0
11	0.0	0.0	0.0	0.5235921189689945	0.0
12	0.0	0.0	0.0	0.6360674630438156	0.0
13	0.0	0.0	0.0	0.6859333791361008	0.0
14	0.0	0.0	0.0	0.7036634826355799	0.0
15	0.0	0.0	0.0	0.7346911637596685	0.0
16	0.0	0.0	0.0	0.8122603665698899	0.0
17	0.0	0.0	0.0	0.9047893442077968	0.0
18	0.0	0.0	0.0	1.0239134770949225	0.0
19	0.0	0.0	0.0	1.0887391680148932	0.0
20	0.0	0.0	0.0	1.153564858934864	0.0
21	0.0	0.0	0.0	1.2427694421666187	0.0
22	0.0	0.0	0.0	1.328095565257862	0.0
23	0.0	0.0	0.0	1.4322599233173023	0.0
24	0.0	0.0	0.0	1.5048425345182952	0.0
25	0.0	0.0	0.0	1.5724385541100596	0.0
26	0.0	0.0	0.0	1.6389264422331065	0.0
27	0.0	0.0	0.0	1.702089935950001	0.0
28	0.0	0.0	0.0	1.7724562842135592	0.0
29	0.0	0.0	0.0	1.855012078633009	0.0
30	0.0	0.0	0.0	1.9608386338955253	0.0
31	0.0	0.0	0.0	2.058354203142661	0.0
32	0.0	0.0	0.0	2.1325990115467297	0.0
33	0.0	0.0	0.0	2.2179251346379734	0.0
34	0.0	0.0	0.0	2.3010349947917823	0.0
35	0.0	0.0	0.0	2.4184969304758317	0.0
36	0.0	0.0	0.0	2.5215531570665544	0.0
37	0.0	0.0	0.0	2.6235012521885595	0.0
38	0.0	0.0	0.0	2.7287737417167173	0.0
39	0.0	0.0	0.0	2.837924691385386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	95	0.0	45.000004	7
TCACGGG	20	7.0174714E-4	45.0	3
GGTACTC	20	7.0174714E-4	45.0	9
GCGACCT	35	1.2043347E-7	45.0	11
CACGGGG	45	3.8016879E-10	45.0	4
GCGCGAG	20	7.0174714E-4	45.0	2
GCGCGAC	35	1.2043347E-7	45.0	9
CGGTTTA	25	3.8777012E-5	45.0	1
GACGTGG	35	1.2043347E-7	45.0	25
ATTACGA	20	7.0174714E-4	45.0	1
TGGCGAC	35	1.2043347E-7	45.0	29
CGACCTC	35	1.2043347E-7	45.0	12
ATGCTAG	25	3.8777012E-5	45.0	2
TACGGGA	20	7.0174714E-4	45.0	4
CGCGACC	35	1.2043347E-7	45.0	10
GTGGCGA	35	1.2043347E-7	45.0	28
CGTTAAG	20	7.0174714E-4	45.0	2
AAACGTA	25	3.8777012E-5	45.0	24
GTTACGG	20	7.0174714E-4	45.0	2
AGCGGGA	25	3.8777012E-5	45.0	4
>>END_MODULE