FastQCFastQC Report
Thu 26 May 2016
SRR1545738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545738_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences691541
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.3726460180958179No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25070.3625236970765291No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.1634031821685193No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.1518348152893321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT254.4409957E-544.018
GTGCAAC207.854305E-444.039
TTAGGCG408.303687E-944.05
AGCGTAA600.044.01
AGGTACG207.854305E-444.07
GGTACGT207.854305E-444.08
CCGGGTA207.854305E-444.029
CGAACGG207.854305E-444.02
ACACGAT254.4409957E-544.013
GGTGTAA454.783942E-1044.01
ATAGGCG1200.044.05
TCCGATG207.854305E-444.01
CATGCCA207.854305E-444.015
CGTGCAC207.854305E-444.023
GCGCGAA207.854305E-444.01
TTTCGAT254.4409957E-544.039
CGGTTAG302.5262489E-644.02
ATTACGA254.4409957E-544.01
CCGGATA302.5262489E-644.020
GCCGGAT302.5262489E-644.019
Produced by FastQC (version 0.11.5)