##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545738_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	691541
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56842038288402	31.0	31.0	33.0	30.0	34.0
2	31.756251617763805	31.0	31.0	34.0	30.0	34.0
3	31.76476882787861	31.0	31.0	34.0	30.0	34.0
4	35.63834393043941	37.0	35.0	37.0	33.0	37.0
5	26.99256298614254	35.0	22.0	37.0	0.0	37.0
6	31.283151685872564	35.0	25.0	37.0	17.0	37.0
7	15.116464533556217	0.0	0.0	32.0	0.0	37.0
8	25.196585596515607	17.0	17.0	35.0	17.0	37.0
9	33.37119274200662	32.0	32.0	37.0	28.0	39.0
10	36.24289232308713	37.0	35.0	39.0	33.0	39.0
11	37.139586806855995	39.0	37.0	39.0	34.0	39.0
12	36.77769069368266	39.0	35.0	39.0	33.0	39.0
13	35.992509482445726	38.0	35.0	39.0	32.0	39.0
14	37.275198433643126	39.0	35.0	41.0	33.0	41.0
15	37.66849977080173	40.0	35.0	41.0	33.0	41.0
16	37.8129843349852	40.0	35.0	41.0	33.0	41.0
17	37.86907934598238	40.0	35.0	41.0	34.0	41.0
18	37.851298766089066	40.0	36.0	41.0	34.0	41.0
19	37.766612536349975	39.0	36.0	41.0	34.0	41.0
20	37.745128633009465	39.0	35.0	41.0	34.0	41.0
21	37.54794148141614	39.0	35.0	41.0	33.0	41.0
22	37.464335447934396	39.0	35.0	41.0	33.0	41.0
23	37.35028870305593	39.0	35.0	41.0	33.0	41.0
24	37.21168231529295	38.0	35.0	41.0	33.0	41.0
25	37.03395315679042	38.0	35.0	41.0	33.0	41.0
26	36.82528729316122	38.0	35.0	40.0	33.0	41.0
27	36.58406515304226	38.0	35.0	40.0	32.0	41.0
28	36.646090398110886	38.0	35.0	40.0	32.0	41.0
29	36.670276961163545	38.0	35.0	40.0	32.0	41.0
30	36.474762884630124	38.0	35.0	40.0	32.0	41.0
31	36.18495360361859	38.0	35.0	40.0	31.0	41.0
32	35.80868524064372	38.0	34.0	40.0	30.0	41.0
33	35.55711230425962	38.0	34.0	40.0	30.0	41.0
34	35.32661982442111	38.0	34.0	40.0	29.0	41.0
35	35.16527581155709	38.0	34.0	40.0	27.0	41.0
36	34.967770529874585	37.0	34.0	40.0	26.0	41.0
37	34.7457403104082	37.0	34.0	40.0	25.0	41.0
38	34.60778898141976	37.0	34.0	40.0	25.0	41.0
39	34.63952824199867	37.0	34.0	40.0	25.0	41.0
40	34.405579712555	37.0	34.0	40.0	24.0	40.0
41	34.26659735286845	36.0	34.0	40.0	23.0	40.0
42	34.266530834758896	36.0	33.0	40.0	23.0	40.0
43	34.119045725416136	36.0	33.0	40.0	23.0	41.0
44	33.92419104579483	36.0	33.0	40.0	23.0	40.0
45	33.6401616100853	35.0	33.0	39.0	23.0	40.0
46	33.69724282435893	36.0	33.0	39.0	23.0	40.0
47	33.55903554525328	35.0	33.0	39.0	23.0	40.0
48	33.605859956242654	35.0	33.0	39.0	23.0	40.0
49	33.54756695553843	36.0	33.0	39.0	23.0	40.0
50	33.51251480389449	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	0.0
14	7.0
15	13.0
16	48.0
17	92.0
18	268.0
19	539.0
20	1106.0
21	1825.0
22	2998.0
23	4779.0
24	7321.0
25	10835.0
26	12974.0
27	13607.0
28	13585.0
29	14865.0
30	17857.0
31	24057.0
32	34634.0
33	72803.0
34	64059.0
35	72168.0
36	78232.0
37	152743.0
38	75403.0
39	14720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.729775964114925	23.43765590181927	28.72367654267787	17.108891591387927
2	33.21928851651601	23.677554909976415	30.4099106199054	12.693245953602172
3	28.330930487129468	22.89553330894336	36.08679745669454	12.686738747232628
4	24.046585813422485	27.30597318163348	34.88093981412527	13.766501190818767
5	16.785121923356677	48.06237085002914	25.087305018791366	10.06520220782282
6	19.527547896653992	39.26506165216524	30.95463609532913	10.252754355851641
7	38.92032431916546	55.543778315385495	4.653809390911023	0.8820879745380246
8	87.7768635554508	2.4209699786419026	8.588789384866551	1.213377081040748
9	84.40728749271554	3.6777284354796027	10.201998146169208	1.7129859256356457
10	61.539084450524264	18.025684666563517	13.634332599224052	6.800898283688168
11	57.84024374549015	15.125205880779301	18.63490378733871	8.39964658639184
12	51.78738498512742	18.683924741989266	20.330826371827555	9.197863901055758
13	20.169736863034874	50.02190759477746	20.12201734965823	9.686338192529439
14	11.891268919702519	51.233838629958306	25.76275303994991	11.112139410389261
15	9.995647401961707	20.484251837562777	59.24984924971911	10.270251510756411
16	11.488689752306804	13.54612380177025	57.83576100332446	17.129425442598485
17	12.450310249139243	13.646045570689228	30.23725274423353	43.666391435938
18	19.80186279627672	20.41816754176542	39.04989002821235	20.730079633745504
19	30.04623008614095	23.253285054682223	26.677087837163665	20.023397022013157
20	35.157712991709815	20.59183764953922	24.747050427957273	19.503398930793693
21	20.403996292338416	29.174119828036225	28.975866940644156	21.446016938981202
22	21.790465062809	24.013615967816804	26.286800059577086	27.909118909797105
23	17.00896982246895	31.97424303114349	24.995914920445784	26.020872225941773
24	19.320040315758575	21.724091557839664	44.656932849968406	14.298935276433355
25	13.738303296550747	22.247415554536897	43.18890709299954	20.82537405591281
26	13.678147788778974	37.8508288011846	29.35502016510952	19.11600324492691
27	15.174082230843869	35.683929080126845	31.486347158013768	17.655641531015515
28	12.0167857003417	27.540521820109003	45.315895948324105	15.126796531225192
29	11.648477819825578	22.983019083467212	44.76900140411053	20.59950169259668
30	15.702322783464757	30.709965135834317	35.250982949673265	18.336729131027663
31	31.82732477177781	25.642442024406364	24.56802995050185	17.962203253313977
32	32.65518602656965	26.486209783657078	26.434730551044694	14.42387363872858
33	29.06566638854385	26.64541943283189	26.85668673296305	17.432227445661212
34	17.76771008515764	27.37466035997866	30.178832491493633	24.678797063370066
35	17.447700136362123	25.402109202491246	33.99740579372734	23.152784867419285
36	32.423818688985904	22.765822995310472	27.413125179851956	17.39723313585167
37	19.15909541155188	32.846497893834204	30.603536160545797	17.390870534068117
38	19.24556895397381	34.24728251831778	23.75419534055103	22.752953187157377
39	18.562456889757804	33.57226831091722	27.69336886750026	20.17190593182472
40	24.371946131899627	25.977057036386853	23.976018775459444	25.674978056254076
41	15.43480429938355	23.558545335706775	27.640009775270013	33.366640589639665
42	22.521875058745614	25.54382169676129	22.85142891021646	29.08287433427664
43	23.216700094426795	26.365031140597594	23.976307984631426	26.441960780344186
44	19.346502954994715	31.657269778653763	28.578204329172095	20.41802293717943
45	15.10134612409098	40.869160324550535	21.15695237158751	22.872541179770973
46	21.99088701899092	34.4848678530991	25.37608037701308	18.14816475089691
47	21.063248599866096	27.789386312597518	26.75690956863006	24.390455518906325
48	23.753183108449104	21.653090706118654	31.762108103496395	22.83161808193585
49	20.115220934116703	21.27625115502913	34.33520210659961	24.273325804254554
50	17.56830036107765	34.709294170555324	26.543762408881033	21.17864305948599
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	618.0
2	773.0
3	4285.0
4	7797.0
5	6022.5
6	4248.0
7	4595.5
8	4943.0
9	5513.0
10	6083.0
11	6119.0
12	6155.0
13	5886.0
14	5617.0
15	5114.0
16	4611.0
17	4211.5
18	3812.0
19	3542.5
20	3273.0
21	3060.0
22	2847.0
23	2731.5
24	2616.0
25	3301.0
26	3986.0
27	4750.5
28	5515.0
29	6447.5
30	7380.0
31	8144.0
32	8908.0
33	10357.0
34	11806.0
35	13716.0
36	15626.0
37	20158.0
38	24690.0
39	36952.0
40	49214.0
41	64918.5
42	80623.0
43	83615.0
44	86607.0
45	80486.5
46	74366.0
47	68071.0
48	61776.0
49	57448.0
50	53120.0
51	47229.0
52	41338.0
53	35502.0
54	29666.0
55	26235.5
56	22805.0
57	20467.0
58	18129.0
59	16402.0
60	14675.0
61	12631.5
62	10588.0
63	8667.0
64	6746.0
65	5429.0
66	4112.0
67	3334.5
68	2557.0
69	2112.5
70	1668.0
71	1353.5
72	1039.0
73	868.0
74	697.0
75	539.5
76	382.0
77	239.0
78	96.0
79	93.0
80	90.0
81	78.5
82	67.0
83	44.5
84	22.0
85	12.5
86	3.0
87	4.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	691541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.200545412274863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75787401225048	17.3442345094634
2	6.20243547562834	2.8779977183803993
3	2.740159458358072	1.9071958185153286
4	1.8286741169201721	1.6970494757543235
5	1.3124679260559482	1.5224985860307618
6	1.025513885798696	1.4275488887034666
7	0.8848393498110565	1.437012886250143
8	0.7150299866637629	1.3271268541384738
9	0.6208940205114979	1.2964571927178232
>10	8.424241986066098	43.97306062303038
>50	1.3811826829305947	20.84458357141294
>100	0.10413171938838434	3.2685964682151187
>500	0.0	0.0
>1k	0.002555379616892867	1.0766374073874132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2577	0.3726460180958179	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2507	0.3625236970765291	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1130	0.1634031821685193	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1050	0.1518348152893321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01547269070091289	0.0
2	0.0	0.0	0.0	0.05032239592446435	0.0
3	0.0	0.0	0.0	0.06579508662537724	0.0
4	0.0	0.0	0.0	0.08791958828182277	0.0
5	0.0	0.0	0.0	0.1370851475183684	0.0
6	0.0	0.0	0.0	0.2304997100678051	0.0
7	0.0	0.0	0.0	0.29166744994150745	0.0
8	0.0	0.0	0.0	0.5936018254882935	0.0
9	0.0	0.0	0.0	0.724324371223109	0.0
10	0.0	0.0	0.0	0.9302413016726413	0.0
11	0.0	0.0	0.0	1.1497510632052186	0.0
12	0.0	0.0	0.0	1.3443888359475433	0.0
13	0.0	0.0	0.0	1.430573169197488	0.0
14	0.0	0.0	0.0	1.4618077597712933	0.0
15	0.0	0.0	0.0	1.5090934593899712	0.0
16	0.0	0.0	0.0	1.624777128181843	0.0
17	0.0	0.0	0.0	1.76330832156011	0.0
18	0.0	0.0	0.0	1.9887468711182708	0.0
19	0.0	0.0	0.0	2.069725439272581	0.0
20	0.0	0.0	0.0	2.1499809844969424	0.0
21	0.0	0.0	0.0	2.263206375326987	0.0
22	0.0	0.0	0.0	2.371081396475408	0.0
23	0.0	0.0	0.0	2.514818354949309	0.0
24	0.0	0.0	0.0	2.6147401238682884	0.0
25	0.0	0.0	0.0	2.6890668810670664	0.0
26	0.0	0.0	0.0	2.7740943776290923	0.0
27	0.0	0.0	0.0	2.8497225761017786	0.0
28	0.0	0.0	0.0	2.92072342782279	0.0
29	0.0	0.0	0.0	3.010089061964511	0.0
30	0.0	0.0	0.0	3.12895403164816	0.0
31	0.0	0.0	0.0	3.238275098656479	0.0
32	0.0	0.0	0.0	3.332123474963885	0.0
33	0.0	0.0	0.0	3.42683947878723	0.0
34	0.0	0.0	0.0	3.531967012801844	0.0
35	0.0	0.0	0.0	3.690019825288739	0.0
36	0.0	0.0	0.0	3.8064265170105607	0.0
37	0.0	0.0	0.0	3.916326002362839	0.0
38	0.0	0.0	0.0	4.021164327205473	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	25	4.4409957E-5	44.0	18
GTGCAAC	20	7.854305E-4	44.0	39
TTAGGCG	40	8.303687E-9	44.0	5
AGCGTAA	60	0.0	44.0	1
AGGTACG	20	7.854305E-4	44.0	7
GGTACGT	20	7.854305E-4	44.0	8
CCGGGTA	20	7.854305E-4	44.0	29
CGAACGG	20	7.854305E-4	44.0	2
ACACGAT	25	4.4409957E-5	44.0	13
GGTGTAA	45	4.783942E-10	44.0	1
ATAGGCG	120	0.0	44.0	5
TCCGATG	20	7.854305E-4	44.0	1
CATGCCA	20	7.854305E-4	44.0	15
CGTGCAC	20	7.854305E-4	44.0	23
GCGCGAA	20	7.854305E-4	44.0	1
TTTCGAT	25	4.4409957E-5	44.0	39
CGGTTAG	30	2.5262489E-6	44.0	2
ATTACGA	25	4.4409957E-5	44.0	1
CCGGATA	30	2.5262489E-6	44.0	20
GCCGGAT	30	2.5262489E-6	44.0	19
>>END_MODULE