##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545737_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	268067
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.025139237578664	31.0	31.0	33.0	30.0	33.0
2	31.191317842181245	31.0	31.0	33.0	30.0	34.0
3	31.104126953336294	31.0	31.0	34.0	28.0	34.0
4	35.00518900125715	35.0	35.0	37.0	32.0	37.0
5	32.76252205605315	35.0	35.0	37.0	28.0	37.0
6	33.96350912271932	35.0	35.0	37.0	28.0	37.0
7	15.671940223899249	0.0	0.0	35.0	0.0	37.0
8	25.136600178313632	17.0	17.0	35.0	17.0	37.0
9	32.92611175564318	32.0	32.0	37.0	28.0	39.0
10	35.69880664162318	37.0	35.0	38.0	32.0	39.0
11	36.56370608840327	37.0	35.0	39.0	32.0	39.0
12	36.1061973312642	37.0	35.0	39.0	32.0	39.0
13	35.379744616084785	37.0	35.0	39.0	30.0	39.0
14	36.377166902304275	38.0	35.0	40.0	30.0	41.0
15	36.93764991587924	39.0	35.0	40.0	31.0	41.0
16	37.13358227607277	39.0	35.0	41.0	32.0	41.0
17	37.14732137861057	39.0	35.0	41.0	32.0	41.0
18	37.020685127225654	38.0	35.0	40.0	32.0	41.0
19	36.90933609881112	38.0	35.0	40.0	32.0	41.0
20	36.96227435678394	38.0	35.0	40.0	32.0	41.0
21	36.73282052621173	38.0	35.0	40.0	31.0	41.0
22	36.79119772295732	38.0	35.0	40.0	32.0	41.0
23	36.78828427221553	38.0	35.0	40.0	32.0	41.0
24	36.62610093745221	38.0	35.0	40.0	32.0	41.0
25	36.37644693304286	38.0	35.0	40.0	31.0	41.0
26	36.26723916035916	38.0	35.0	40.0	31.0	41.0
27	36.1205817948498	38.0	34.0	40.0	31.0	41.0
28	36.12494637534646	38.0	35.0	40.0	31.0	41.0
29	36.18328999839592	38.0	35.0	40.0	31.0	41.0
30	35.9420928350002	37.0	34.0	40.0	30.0	41.0
31	35.71404163884402	37.0	34.0	40.0	30.0	41.0
32	35.48224884077488	37.0	34.0	40.0	29.0	41.0
33	35.24885942693431	37.0	34.0	40.0	27.0	41.0
34	35.0870640548818	37.0	34.0	40.0	27.0	41.0
35	34.872102869804934	37.0	34.0	40.0	25.0	41.0
36	34.636859441855954	37.0	33.0	40.0	24.0	41.0
37	34.48624411061414	37.0	33.0	40.0	23.0	41.0
38	34.46983403402881	37.0	33.0	40.0	23.0	41.0
39	34.4416545117452	37.0	33.0	40.0	23.0	41.0
40	34.24787832892523	36.0	33.0	40.0	23.0	41.0
41	34.13826021106664	36.0	33.0	40.0	23.0	41.0
42	34.0894291352535	36.0	33.0	40.0	23.0	41.0
43	33.98263120786967	36.0	33.0	40.0	23.0	41.0
44	33.67652116821541	36.0	33.0	40.0	21.0	41.0
45	33.60303580821213	36.0	33.0	40.0	20.0	41.0
46	33.66751968724237	36.0	33.0	40.0	22.0	41.0
47	33.65290020778387	35.0	33.0	40.0	22.0	41.0
48	33.690954127139854	36.0	33.0	40.0	22.0	41.0
49	33.67075022289204	36.0	33.0	40.0	22.0	41.0
50	33.486035207616005	35.0	33.0	40.0	20.0	41.0
51	33.24790817221068	35.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	10.0
16	35.0
17	84.0
18	201.0
19	375.0
20	696.0
21	1067.0
22	1636.0
23	2453.0
24	3575.0
25	4660.0
26	5390.0
27	5635.0
28	5944.0
29	6688.0
30	8232.0
31	10641.0
32	14328.0
33	22939.0
34	28981.0
35	27122.0
36	27109.0
37	41974.0
38	41292.0
39	6995.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.233859445586365	24.035409058183216	29.073328682754685	16.657402813475734
2	32.645942991863976	23.437424226033045	31.327242816161633	12.58938996594135
3	28.81593034577177	22.388432742560628	35.92198965184077	12.873647259826834
4	24.539760582242497	27.992255667426424	33.57071179966202	13.89727195066905
5	20.388186535455688	36.58451058877072	30.56586599618752	12.461436879586074
6	19.658518206269328	39.89562310914809	30.362185573009732	10.083673111572853
7	38.46612973622266	55.95429500833746	4.94689760395722	0.6326776514826518
8	88.55845740057524	2.6008423267317498	7.743959532504934	1.0967407401880873
9	85.1272256562725	3.7848746768531747	9.308493772079368	1.7794058947949583
10	62.060231210854	18.41330712098095	12.876258547303474	6.650203120861575
11	58.44434413784614	15.440542849362288	17.886573132836194	8.228539879955385
12	52.4738218430467	18.937056780580974	19.604054210328016	8.98506716604431
13	20.307609664747993	50.7768580242999	19.139618080554488	9.775914230397625
14	11.886580593657555	51.90344204993528	25.15565138566105	11.054325970746119
15	9.973252955417863	20.906340579034346	58.942727004815964	10.177679460731833
16	11.573972178597142	13.703290595261633	57.59903307755151	17.12370414858972
17	12.553578023404595	13.770811028586136	29.68250474694759	43.99310620106168
18	19.65217650811178	20.46950948829957	39.174534724527824	20.703779279060832
19	31.103791216375008	23.010292203068637	26.004692856636584	19.881223723919767
20	36.067475668396334	20.657895227685614	24.16000477492567	19.11462432899238
21	20.59298608183775	29.995859244144185	28.138860807186262	21.2722938668318
22	21.722927477085953	24.07383228819661	25.457441609746816	28.745798624970625
23	17.027459553022194	32.73509980713777	24.344287062562717	25.89315357727732
24	18.972868723117728	21.840062372466583	44.909668105361725	14.277400799053968
25	13.770811028586136	22.403354385284278	43.39027183502632	20.43556275110327
26	13.667105611656789	38.444493354273376	28.86442568462362	19.02397534944622
27	15.011172579989331	37.15190605333741	30.414784363610593	17.42213700306267
28	12.02572491205557	27.887431127292807	44.98614152432041	15.100702436331215
29	11.738856330693446	23.32252757706096	44.488131698418684	20.45048439382692
30	15.698314227413295	31.725277635814926	34.35409804265351	18.22231009411826
31	32.325873755441734	25.86927894891949	24.02459086720857	17.780256428430206
32	33.82437972596403	25.992382501389578	25.96738874982747	14.215849022818922
33	30.25400366326329	26.32326992878646	26.103175698612663	17.31955070933759
34	17.943275375186055	27.14396027858707	29.55828207127323	25.35448227495365
35	17.707886461220514	25.01837227260349	34.349994590904515	22.92374667527148
36	33.2670563702358	22.946875221493134	26.73548030902722	17.050588099243846
37	19.323154286055352	33.87772459870107	29.83731679020543	16.961804325038145
38	19.16573095532087	35.350863776593165	23.26396012937064	22.219445138715322
39	18.75575882148866	34.64469703469655	27.016380233299884	19.58316391051491
40	25.38320643719667	25.76594657305823	23.45495715623333	25.39588983351177
41	15.479712161511861	23.64371593668747	26.868282929267686	34.00828897253299
42	22.12767703596489	25.280247102403504	22.329865294870313	30.262210566761294
43	22.796166629984295	25.86181812755766	23.985048513990908	27.356966728467135
44	19.11126695937956	31.30784468062089	29.207996508335604	20.372891851663947
45	14.9108245326728	41.59892862605244	21.144340780476522	22.345906060798235
46	21.521858341384803	35.44412404361596	24.82625612253653	18.207761492462705
47	20.741083385869953	27.918020494876284	26.208746320882465	25.1321497983713
48	23.163612082054115	21.893407245203626	32.54634102668363	22.396639646058635
49	20.194951262184453	21.460306565149757	34.77563444959655	23.569107723069234
50	17.58963244263561	35.886177709304015	25.94575236787818	20.578437480182192
51	16.502217729149802	37.857699754165935	22.41678386373556	23.223298652948703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	238.0
2	284.0
3	1446.5
4	2609.0
5	2079.5
6	1550.0
7	1626.5
8	1703.0
9	1851.5
10	2000.0
11	1980.0
12	1960.0
13	1911.5
14	1863.0
15	1763.0
16	1663.0
17	1508.5
18	1354.0
19	1292.5
20	1231.0
21	1147.0
22	1063.0
23	1034.5
24	1006.0
25	1157.0
26	1670.5
27	2033.0
28	2342.5
29	2652.0
30	2930.5
31	3209.0
32	3792.5
33	4376.0
34	5029.0
35	5682.0
36	6993.5
37	8305.0
38	12309.0
39	16313.0
40	22238.5
41	28164.0
42	30377.0
43	32590.0
44	30589.5
45	28589.0
46	26640.5
47	24692.0
48	22932.0
49	21172.0
50	19724.5
51	18277.0
52	15759.5
53	13242.0
54	11712.0
55	10182.0
56	9391.5
57	8601.0
58	7614.0
59	6627.0
60	5719.0
61	4811.0
62	3988.5
63	3166.0
64	2534.5
65	1903.0
66	1595.5
67	1288.0
68	1101.0
69	914.0
70	748.5
71	583.0
72	489.0
73	395.0
74	332.5
75	206.0
76	142.0
77	86.0
78	30.0
79	32.0
80	34.0
81	29.0
82	24.0
83	16.5
84	9.0
85	6.0
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.996728429832842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.18856207577143	22.066125259729844
2	7.090935348770038	4.395915946386538
3	3.747653203677851	3.484949658107861
4	2.5489818514417752	3.1604039288685293
5	1.9424252635632793	3.0104414194958724
6	1.4947287344148654	2.779902039415519
7	1.205892263996534	2.61651005159158
8	1.032590381745535	2.560553891377902
9	0.8965965435902372	2.501240361551403
>10	8.732489288980888	50.16693587797081
>50	0.11192413228710346	2.21399873912119
>100	0.0036104558802291437	0.1372791130575565
>500	0.002406970586819429	0.5323296041661226
>1k	0.0012034852934097145	0.37341410915927736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1001	0.37341410915927736	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.29022595097494286	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.24210365319117982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01566772485982982	0.0
2	0.0	0.0	0.0	0.06005961196268097	0.0
3	0.0	0.0	0.0	0.07199692614159893	0.0
4	0.0	0.0	0.0	0.09288722595470536	0.0
5	0.0	0.0	0.0	0.14436689335128905	0.0
6	0.0	0.0	0.0	0.21039516240342898	0.0
7	0.0	0.0	0.0	0.24396885853163575	0.0
8	0.0	0.0	0.0	0.48756467599518033	0.0
9	0.0	0.0	0.0	0.5901509697202565	0.0
10	0.0	0.0	0.0	0.7550351218165607	0.0
11	0.0	0.0	0.0	0.9270070542065976	0.0
12	0.0	0.0	0.0	1.075850440374981	0.0
13	0.0	0.0	0.0	1.15418906467413	0.0
14	0.0	0.0	0.0	1.1773176108957835	0.0
15	0.0	0.0	0.0	1.2157408409091757	0.0
16	0.0	0.0	0.0	1.3131045596809752	0.0
17	0.0	0.0	0.0	1.4246438390402398	0.0
18	0.0	0.0	0.0	1.6100452498815594	0.0
19	0.0	0.0	0.0	1.6865186688402527	0.0
20	0.0	0.0	0.0	1.7562773485733045	0.0
21	0.0	0.0	0.0	1.857744519094107	0.0
22	0.0	0.0	0.0	1.951750868253086	0.0
23	0.0	0.0	0.0	2.0849265295616393	0.0
24	0.0	0.0	0.0	2.164384277065062	0.0
25	0.0	0.0	0.0	2.236381203206661	0.0
26	0.0	0.0	0.0	2.309870293620625	0.0
27	0.0	0.0	0.0	2.3781368090813118	0.0
28	0.0	0.0	0.0	2.446030283473908	0.0
29	0.0	0.0	0.0	2.52548803097733	0.0
30	0.0	0.0	0.0	2.6265821604300417	0.0
31	0.0	0.0	0.0	2.733271905904121	0.0
32	0.0	0.0	0.0	2.8089992427266317	0.0
33	0.0	0.0	0.0	2.8832344152767777	0.0
34	0.0	0.0	0.0	2.9832094215252156	0.0
35	0.0	0.0	0.0	3.1227267809913193	0.0
36	0.0	0.0	0.0	3.226432197920669	0.0
37	0.0	0.0	0.0	3.3237959166924687	0.0
38	0.0	0.0	0.0	3.4196674711919037	0.0
39	0.0	0.0	0.0	3.512554697146609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	40	6.7811925E-9	45.000004	25
TTGATAA	40	6.7811925E-9	45.000004	1
CGGAATT	20	7.0235983E-4	45.000004	17
AGGTACT	20	7.0235983E-4	45.000004	7
TCCATAT	20	7.0235983E-4	45.000004	41
CGCGGTG	20	7.0235983E-4	45.000004	23
GTTTCGA	20	7.0235983E-4	45.000004	25
CGACGGT	40	6.7811925E-9	45.000004	28
GCGAATC	20	7.0235983E-4	45.000004	27
GAGTATA	20	7.0235983E-4	45.000004	1
GAGTACG	20	7.0235983E-4	45.000004	1
GGGTACC	20	7.0235983E-4	45.000004	7
GCGTTGG	20	7.0235983E-4	45.000004	2
CTGGTAA	20	7.0235983E-4	45.000004	1
GTTACGG	20	7.0235983E-4	45.000004	2
CTACGGG	20	7.0235983E-4	45.000004	3
CGAGGCA	20	7.0235983E-4	45.000004	5
AACGCGG	20	7.0235983E-4	45.000004	2
TCGTCCG	20	7.0235983E-4	45.000004	34
CTCATGT	20	7.0235983E-4	45.000004	21
>>END_MODULE