FastQCFastQC Report
Thu 26 May 2016
SRR1545736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545736_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences833149
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.55608300556083No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23690.2843428966487387No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.23177126780443832No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19040.22853055095787186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT254.4417826E-544.024
CATGCGC207.855234E-444.035
CGACGCA207.855234E-444.017
TCATTAC207.855234E-444.037
CGATTAC351.4454417E-744.010
TAGCCGT207.855234E-444.044
CATTACC207.855234E-444.038
ATCCGGC302.5268746E-644.016
TGGCACG254.4417826E-544.032
AATGCGC302.5268746E-644.034
CTACGTT207.855234E-444.041
ATATACG254.4417826E-544.01
GTAGACC254.4417826E-544.016
TACGAAC207.855234E-444.012
CGCTACG207.855234E-444.033
CGCTAAG207.855234E-444.02
GTGTCGT207.855234E-444.027
ACGTGAC254.4417826E-544.025
GTCCGTT254.4417826E-544.09
ATAAGTC207.855234E-444.044
Produced by FastQC (version 0.11.5)