##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545736_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	833149
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.536398651381685	31.0	31.0	33.0	30.0	34.0
2	31.731906297673046	31.0	31.0	34.0	30.0	34.0
3	31.719629982152053	31.0	31.0	34.0	30.0	34.0
4	35.6386948793073	37.0	35.0	37.0	33.0	37.0
5	27.00608654634405	35.0	22.0	37.0	0.0	37.0
6	31.292414682127685	35.0	26.0	37.0	17.0	37.0
7	15.072474431344213	0.0	0.0	32.0	0.0	37.0
8	25.142580738859436	17.0	17.0	35.0	17.0	37.0
9	33.2673915470102	32.0	32.0	37.0	28.0	39.0
10	36.15031524973324	37.0	35.0	39.0	32.0	39.0
11	37.10594623530725	39.0	37.0	39.0	34.0	39.0
12	36.90568673790642	39.0	35.0	39.0	33.0	39.0
13	36.279579042884286	39.0	35.0	39.0	32.0	39.0
14	37.576386696737316	40.0	35.0	41.0	33.0	41.0
15	37.90252643884828	40.0	35.0	41.0	33.0	41.0
16	38.00968734284024	40.0	35.0	41.0	33.0	41.0
17	38.05100648263396	40.0	35.0	41.0	34.0	41.0
18	38.020450123567336	40.0	36.0	41.0	34.0	41.0
19	37.92870182884454	40.0	36.0	41.0	34.0	41.0
20	37.886744147805494	40.0	35.0	41.0	34.0	41.0
21	37.72809425444908	40.0	35.0	41.0	33.0	41.0
22	37.60251887717563	39.0	35.0	41.0	33.0	41.0
23	37.48865809116977	39.0	35.0	41.0	33.0	41.0
24	37.3171305492775	39.0	35.0	41.0	33.0	41.0
25	37.135105485333355	38.0	35.0	41.0	33.0	41.0
26	36.880615592168986	38.0	35.0	40.0	33.0	41.0
27	36.65405347662903	38.0	35.0	40.0	32.0	41.0
28	36.69254599117325	38.0	35.0	40.0	32.0	41.0
29	36.68934128229164	38.0	35.0	40.0	32.0	41.0
30	36.44706889163883	38.0	35.0	40.0	32.0	41.0
31	36.145819055175004	38.0	35.0	40.0	31.0	41.0
32	35.74427503363744	38.0	35.0	40.0	30.0	41.0
33	35.4326561035301	38.0	34.0	40.0	29.0	41.0
34	35.1069364543437	38.0	34.0	40.0	27.0	41.0
35	34.871309933757345	38.0	34.0	40.0	24.0	41.0
36	34.65297323768017	38.0	34.0	40.0	23.0	41.0
37	34.46448834482187	37.0	34.0	40.0	23.0	41.0
38	34.32618295166891	37.0	33.0	40.0	23.0	41.0
39	34.31724457449988	37.0	34.0	40.0	23.0	41.0
40	34.0661646356174	37.0	33.0	40.0	23.0	40.0
41	33.90837773315457	37.0	33.0	40.0	22.0	40.0
42	33.90333781832541	37.0	33.0	40.0	21.0	40.0
43	33.76178210620189	36.0	33.0	40.0	21.0	41.0
44	33.52568388127454	36.0	33.0	40.0	20.0	40.0
45	33.32292903190186	36.0	33.0	39.0	20.0	40.0
46	33.3255300072376	36.0	33.0	39.0	20.0	40.0
47	33.19251538440303	35.0	33.0	39.0	20.0	40.0
48	33.16781992176669	35.0	33.0	39.0	20.0	40.0
49	33.05562270374207	35.0	33.0	39.0	18.0	40.0
50	33.0739027472877	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	5.0
15	22.0
16	36.0
17	138.0
18	357.0
19	743.0
20	1421.0
21	2513.0
22	3993.0
23	6428.0
24	9982.0
25	14757.0
26	18539.0
27	18881.0
28	17626.0
29	18292.0
30	21250.0
31	28212.0
32	39707.0
33	78435.0
34	73123.0
35	85149.0
36	98993.0
37	188176.0
38	87260.0
39	19103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.76700566165236	22.65045027960185	27.597824638810103	18.98471941993569
2	34.556123814587785	23.567093040980666	29.628913915758165	12.247869228673384
3	27.760100534238173	22.615162473939236	36.82150491688761	12.803232074934975
4	25.84003581592248	25.358729350932425	34.99914181016841	13.802093022976683
5	16.441836934329874	47.870068859231665	25.292114615753004	10.39597959068546
6	19.176161766982858	38.99434554923549	31.865008539889022	9.96448414389263
7	37.70418016465242	55.645868866193204	5.739429561819074	0.9105214073353025
8	84.43555714524052	3.0251491629948544	11.086612358653735	1.452681333110884
9	81.31258634409932	4.25410100714278	12.14056549308707	2.2927471556708343
10	54.74963061829277	19.64678586903423	16.59499081196761	9.008592700705396
11	50.2167079357954	17.000800577087652	22.036634503552186	10.745856983564765
12	44.355211372755655	19.33051591011932	25.2550264118423	11.059246305282729
13	21.25490158423043	42.07614724377032	25.145322145258532	11.523629026740716
14	13.319466265938026	44.08887245858784	29.919138113350673	12.672523162123461
15	11.825375773121015	21.57321199449318	53.895881769047314	12.70553046333849
16	13.093576299077355	14.683207925593141	52.85249097100279	19.370724804326716
17	14.435833206305235	15.448017101382828	33.438436582171974	36.67771311013996
18	19.94949282781351	20.90094328865545	38.07170146036303	21.077862423168003
19	28.523589417979256	22.402595454114447	29.00897678566499	20.064838342241302
20	30.635096483342117	21.113870388129854	28.018757749214124	20.232275379313904
21	20.526220399952468	27.187093785145272	31.25635390548389	21.03033190941836
22	21.26282333652204	23.84783514113322	28.246208061223143	26.6431334611216
23	17.741484416352897	29.709211677623088	27.91289433222629	24.636409573797724
24	18.433557502919644	22.141657734690913	43.82169335857092	15.603091403818524
25	16.007340823790223	23.237860214679486	40.46683126307539	20.2879676984549
26	14.704692678020379	33.74354407194872	31.222146338770134	20.32961691126077
27	16.137569630402247	32.543638652870015	33.18241995129323	18.136371765434514
28	13.514029303281886	26.925675959522245	43.50434316070715	16.05595157648872
29	13.382240151521515	23.600460421845312	42.35376865362618	20.66353077300699
30	17.686992362710633	29.234626699425913	34.99061992512743	18.087761012736017
31	29.691087668592292	24.834453381087897	27.180732378002016	18.293726572317798
32	30.487703880098277	24.63628954724785	28.770243977967922	16.10576259468594
33	28.06592818331415	25.31395944782986	27.07006789901926	19.550044469836728
34	18.41711386558707	25.768980098397765	31.46676044741097	24.3471455886042
35	19.878197057189052	24.30357595100036	32.884033948309366	22.93419304350122
36	30.53271383630059	23.80678606107671	27.08135039470731	18.57914970791539
37	20.416276080268954	32.788132734960975	28.498263815956086	18.29732736881398
38	20.00914602310031	33.89225696724115	24.585998422851134	21.512598586807403
39	20.186665290362228	31.76958743274012	27.013175314379538	21.03057196251811
40	23.198491506321197	25.78962466497589	26.26456972282269	24.74731410588022
41	16.825921893922935	23.84075357469072	27.815432773729547	31.517891757656795
42	21.254301451481066	25.469753909564798	24.03987762092975	29.236067018024386
43	21.65783071215353	25.755417098262136	26.254487492633373	26.332264696950965
44	19.03993163287719	30.27117598412769	28.763282438075304	21.925609944919817
45	16.537378068028648	37.88421998946167	23.44094513706432	22.137456805445368
46	21.329558098251333	32.34955572172565	27.1759313160071	19.14495486401592
47	21.918768431577064	27.523408177888946	27.475877664139308	23.08194572639468
48	23.04089664633817	23.398575764959208	31.45619811102216	22.104329477680462
49	20.395871566790575	23.289951737324298	33.340975023675234	22.973201672209893
50	18.398629776906652	32.46190057240661	27.958384394628094	21.18108525605864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	490.0
1	793.5
2	1097.0
3	7299.5
4	13502.0
5	10166.0
6	6830.0
7	7263.0
8	7696.0
9	8261.0
10	8826.0
11	8983.0
12	9140.0
13	8604.5
14	8069.0
15	7554.0
16	7039.0
17	6375.0
18	5711.0
19	5235.5
20	4760.0
21	4459.5
22	4159.0
23	4284.5
24	4410.0
25	4803.0
26	5196.0
27	6475.5
28	7755.0
29	9133.0
30	10511.0
31	11480.5
32	12450.0
33	14187.0
34	15924.0
35	16776.5
36	17629.0
37	23688.0
38	29747.0
39	42461.5
40	55176.0
41	67595.0
42	80014.0
43	82997.0
44	85980.0
45	83958.5
46	81937.0
47	76025.5
48	70114.0
49	66658.0
50	63202.0
51	57201.0
52	51200.0
53	44482.0
54	37764.0
55	34152.5
56	30541.0
57	28320.5
58	26100.0
59	23367.0
60	20634.0
61	18356.0
62	16078.0
63	13444.5
64	10811.0
65	8947.0
66	7083.0
67	6273.0
68	5463.0
69	4786.5
70	4110.0
71	3581.0
72	3052.0
73	2333.0
74	1614.0
75	1167.0
76	720.0
77	545.5
78	371.0
79	247.5
80	124.0
81	83.5
82	43.0
83	47.5
84	52.0
85	34.5
86	17.0
87	11.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	833149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.701001256306952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8391519960797	18.733029887324186
2	6.110309008778312	3.0186150100451354
3	2.591281428711192	1.9202173747812006
4	1.6846891046114163	1.6645403075797292
5	1.2388668327178096	1.5300625595679815
6	1.0246415242269957	1.5185802946317173
7	0.7904273063465911	1.3667042120960526
8	0.7178228376193231	1.4184754251072575
9	0.6634059089739655	1.4748111171906637
>10	8.13098194143518	44.74890150803981
>50	1.1085608509508784	17.900571921401582
>100	0.09784386036587149	3.355422889388938
>500	0.0	0.0
>1k	0.002017399182801474	1.350067492845768
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4633	0.55608300556083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2369	0.2843428966487387	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1931	0.23177126780443832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1904	0.22853055095787186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011282495688046196	0.0
2	0.0	0.0	0.0	0.033967513614011416	0.0
3	0.0	0.0	0.0	0.045129982752184786	0.0
4	0.0	0.0	0.0	0.06529444313082054	0.0
5	0.0	0.0	0.0	0.11714591267588391	0.0
6	0.0	0.0	0.0	0.19228253289627667	0.0
7	0.0	0.0	0.0	0.2440139758914672	0.0
8	0.0	0.0	0.0	0.5716864570442982	0.0
9	0.0	0.0	0.0	0.7182388744390259	0.0
10	0.0	0.0	0.0	1.003782036586493	0.0
11	0.0	0.0	0.0	1.1811812772985384	0.0
12	0.0	0.0	0.0	1.3930281378240867	0.0
13	0.0	0.0	0.0	1.4650440677477858	0.0
14	0.0	0.0	0.0	1.4859286874256585	0.0
15	0.0	0.0	0.0	1.528538112630514	0.0
16	0.0	0.0	0.0	1.6178378657359007	0.0
17	0.0	0.0	0.0	1.7249015482224668	0.0
18	0.0	0.0	0.0	1.8990600720879458	0.0
19	0.0	0.0	0.0	1.966875072766096	0.0
20	0.0	0.0	0.0	2.050773631127205	0.0
21	0.0	0.0	0.0	2.145234525877124	0.0
22	0.0	0.0	0.0	2.2364547037804763	0.0
23	0.0	0.0	0.0	2.343638412816915	0.0
24	0.0	0.0	0.0	2.4276569977278974	0.0
25	0.0	0.0	0.0	2.4862299540658395	0.0
26	0.0	0.0	0.0	2.5500840785981858	0.0
27	0.0	0.0	0.0	2.6126179110819314	0.0
28	0.0	0.0	0.0	2.678512486962116	0.0
29	0.0	0.0	0.0	2.76133080637437	0.0
30	0.0	0.0	0.0	2.8637134534158957	0.0
31	0.0	0.0	0.0	2.9581743481658145	0.0
32	0.0	0.0	0.0	3.041232720677814	0.0
33	0.0	0.0	0.0	3.1272917569366343	0.0
34	0.0	0.0	0.0	3.2235530499346456	0.0
35	0.0	0.0	0.0	3.339498697111801	0.0
36	0.0	0.0	0.0	3.4363601228591762	0.0
37	0.0	0.0	0.0	3.5355020530541355	0.0
38	0.0	0.0	0.0	3.628042523006089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	25	4.4417826E-5	44.0	24
CATGCGC	20	7.855234E-4	44.0	35
CGACGCA	20	7.855234E-4	44.0	17
TCATTAC	20	7.855234E-4	44.0	37
CGATTAC	35	1.4454417E-7	44.0	10
TAGCCGT	20	7.855234E-4	44.0	44
CATTACC	20	7.855234E-4	44.0	38
ATCCGGC	30	2.5268746E-6	44.0	16
TGGCACG	25	4.4417826E-5	44.0	32
AATGCGC	30	2.5268746E-6	44.0	34
CTACGTT	20	7.855234E-4	44.0	41
ATATACG	25	4.4417826E-5	44.0	1
GTAGACC	25	4.4417826E-5	44.0	16
TACGAAC	20	7.855234E-4	44.0	12
CGCTACG	20	7.855234E-4	44.0	33
CGCTAAG	20	7.855234E-4	44.0	2
GTGTCGT	20	7.855234E-4	44.0	27
ACGTGAC	25	4.4417826E-5	44.0	25
GTCCGTT	25	4.4417826E-5	44.0	9
ATAAGTC	20	7.855234E-4	44.0	44
>>END_MODULE