FastQCFastQC Report
Thu 26 May 2016
SRR1545735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545735_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences320071
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.3986615469692662No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.3464856235022854No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9560.2986837295475066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGC253.8844788E-545.04
GTGCACT207.0256507E-445.039
CTCACGA800.045.024
CATATAG207.0256507E-445.02
AGTTTCG207.0256507E-445.031
CTCAACT207.0256507E-445.023
ATAGGCC351.2082637E-745.05
ACGTTAG253.8844788E-545.02
TATGGGT207.0256507E-445.04
CCGTGAG207.0256507E-445.024
TGTATGA207.0256507E-445.01
GTGATAC253.8844788E-545.09
AAGGTAC207.0256507E-445.06
CGATTCT351.2082637E-745.010
AGGAACG207.0256507E-445.07
GGATAGT253.8844788E-545.08
GTCAGAC207.0256507E-445.044
GCCGATG207.0256507E-445.09
TACTGAC453.8380676E-1045.020
GTGTATG207.0256507E-445.01