##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545735_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	320071
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.006011166272483	31.0	31.0	33.0	30.0	33.0
2	31.15727135541802	31.0	31.0	33.0	30.0	34.0
3	31.04050351328299	31.0	31.0	34.0	28.0	34.0
4	35.00849811448085	35.0	35.0	37.0	32.0	37.0
5	32.78455717637649	35.0	35.0	37.0	28.0	37.0
6	33.98096672300833	35.0	35.0	37.0	28.0	37.0
7	15.722177266918901	0.0	0.0	35.0	0.0	37.0
8	25.11658038372736	17.0	17.0	35.0	17.0	37.0
9	32.80580246257862	32.0	32.0	37.0	27.0	39.0
10	35.60031368040216	37.0	35.0	37.0	32.0	39.0
11	36.529151344545426	37.0	35.0	39.0	32.0	39.0
12	36.194566205623126	37.0	35.0	39.0	32.0	39.0
13	35.67975230495734	37.0	35.0	39.0	31.0	39.0
14	36.69422409402914	39.0	35.0	40.0	31.0	41.0
15	37.13649471523506	39.0	35.0	41.0	32.0	41.0
16	37.2809657857163	39.0	35.0	41.0	32.0	41.0
17	37.23982178954044	39.0	35.0	41.0	32.0	41.0
18	37.14706424512061	39.0	35.0	40.0	32.0	41.0
19	37.01921448678574	38.0	35.0	40.0	32.0	41.0
20	37.035254677868345	39.0	35.0	40.0	32.0	41.0
21	36.8714941372383	38.0	35.0	40.0	32.0	41.0
22	36.88965260832753	38.0	35.0	40.0	32.0	41.0
23	36.88500676412421	38.0	35.0	40.0	32.0	41.0
24	36.699160498764336	38.0	35.0	40.0	32.0	41.0
25	36.44586982263311	38.0	35.0	40.0	31.0	41.0
26	36.31273373720206	38.0	35.0	40.0	31.0	41.0
27	36.18092235785185	38.0	35.0	40.0	31.0	41.0
28	36.12068572285524	38.0	35.0	40.0	31.0	41.0
29	36.1630575716013	38.0	35.0	40.0	31.0	41.0
30	35.87779898834946	38.0	34.0	40.0	30.0	41.0
31	35.666336531582054	38.0	34.0	40.0	30.0	41.0
32	35.39504672400811	38.0	34.0	40.0	29.0	41.0
33	35.073236875568234	38.0	34.0	40.0	26.0	41.0
34	34.81951192079257	38.0	34.0	40.0	24.0	41.0
35	34.590275282671655	37.0	34.0	40.0	23.0	41.0
36	34.34225531210263	37.0	33.0	40.0	22.0	41.0
37	34.175873478072056	37.0	33.0	40.0	22.0	41.0
38	34.13065226152947	37.0	33.0	40.0	22.0	41.0
39	34.07670485611005	37.0	33.0	40.0	22.0	41.0
40	33.90091261001465	37.0	33.0	40.0	21.0	41.0
41	33.76776402735643	37.0	33.0	40.0	20.0	41.0
42	33.71721274342255	36.0	33.0	40.0	19.0	41.0
43	33.61084884291298	36.0	33.0	40.0	18.0	41.0
44	33.38623305454102	36.0	33.0	40.0	18.0	41.0
45	33.3097375269862	36.0	33.0	40.0	18.0	41.0
46	33.32141306147698	36.0	32.0	40.0	18.0	41.0
47	33.28956700232136	36.0	32.0	40.0	18.0	41.0
48	33.292278900618925	36.0	32.0	40.0	18.0	41.0
49	33.20865058065242	36.0	32.0	40.0	18.0	41.0
50	33.052650818099735	35.0	32.0	40.0	18.0	41.0
51	32.8740185771282	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	13.0
16	32.0
17	112.0
18	283.0
19	523.0
20	902.0
21	1480.0
22	2217.0
23	3287.0
24	4741.0
25	6279.0
26	7445.0
27	7385.0
28	7411.0
29	7918.0
30	9612.0
31	12261.0
32	16688.0
33	25891.0
34	32458.0
35	31458.0
36	33799.0
37	50989.0
38	48018.0
39	8864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.51697904527433	23.465731040925295	27.92942815812742	18.08786175567296
2	33.806561669129664	23.674747165472663	30.113631038113418	12.405060127284258
3	28.251231757953704	22.66184690271846	36.10823848458623	12.978682854741605
4	26.373523374501286	26.16919371014556	33.427583254965306	14.029699660387852
5	20.408284411896112	36.16635059096263	30.510730431685467	12.91463456545579
6	19.356017883532093	39.79304591793696	31.104036291947722	9.746899906583227
7	37.159880151591366	55.745756410296465	6.432010397693013	0.662353040419157
8	85.20047114546459	3.4695426952144994	9.923423240468521	1.4065629188523796
9	81.96243958371736	4.422768698195088	10.990998872125248	2.623792845962302
10	55.54705049817071	20.01649633987459	15.519056709292625	8.917396452662066
11	51.34329570626517	17.210243977117575	20.94316573510252	10.503294581514727
12	44.922532813032106	19.68063335947337	24.149329367546578	11.24750445994795
13	21.645509902490385	43.13667904933593	23.8784519684695	11.33935907970419
14	13.306422637477311	45.3402526314474	28.841413311421526	12.511911419653766
15	11.81550343517532	21.87733346663709	53.535309353237245	12.77185374495034
16	12.96149916737224	15.001359073455578	52.39899897210306	19.638142787069118
17	14.377747437287352	15.778374173230315	32.804596480155965	37.03928190932636
18	20.02336981482234	21.059702378534762	37.752561150494735	21.164366656148168
19	29.2132058199587	22.502194825523087	28.16031443023579	20.124284924282428
20	31.577368771303867	21.338078113918474	27.20896301133186	19.8755901034458
21	20.739148501426246	28.11657413511377	30.243602200761703	20.900675162698278
22	21.17373957653146	24.217751686344595	27.333622852429617	27.274885884694335
23	17.907901684313792	30.297027846946456	26.955269299624145	24.839801169115603
24	18.058180841125875	22.59436187595877	43.767789021810785	15.579668261104567
25	15.760878055181507	23.869703909445093	40.31605487532454	20.053363160048864
26	14.567705290388695	34.47797519925266	30.69600182459517	20.258317685763473
27	16.0892426992761	33.92403560460023	32.131620796635744	17.855100899487926
28	13.544494815212873	27.804143455670772	42.86798866501495	15.783373064101403
29	13.325793339602775	24.067160098853066	41.925072874455985	20.681973687088178
30	17.76824517060277	30.403254277957075	34.118367487213774	17.710133064226376
31	30.279844159577095	25.366246863977054	26.202936223525402	18.150972752920445
32	31.752329951791946	24.382715085090496	28.07470842406841	15.79024653904915
33	29.10916640370418	25.318132539342837	26.12826529113853	19.44443576581446
34	18.66742066603972	25.768345148420195	30.589462962905106	24.974771222634978
35	20.08616838139038	24.108400948539543	32.815531553936474	22.989899116133607
36	31.316489153968963	24.11839873028172	26.429760896801024	18.135351218948294
37	20.422656223150486	33.840304182509506	27.87475278922489	17.862286805115115
38	19.67969606743504	34.818524639845535	24.07590815787747	21.425871134841955
39	20.43484101964877	32.66337781304773	26.37539795857794	20.526383208725562
40	23.89563565583886	25.79833849364672	25.658057118576817	24.6479687319376
41	16.780651792883454	23.93000303057759	27.30581652195919	31.983528654579764
42	20.963161298586876	25.179100886990696	23.66443695305104	30.19330086137138
43	21.37994382496384	25.353749636799332	26.130139875215185	27.136166663021644
44	18.728032217851663	30.058955669210896	29.25132236285074	21.9616897500867
45	16.369493018736467	38.86325221591459	23.047073930471676	21.72018083487726
46	21.135623033639412	33.35353718393731	26.551296431104348	18.959543351318924
47	21.49897991383162	27.88725001640261	26.89871934664496	23.715050723120807
48	22.632165988171373	23.779099012406622	31.817003102436647	21.771731896985354
49	20.373292175798493	23.61101130686629	33.695648777927396	22.32004773940782
50	18.51807880126597	33.63628694883323	27.232395312290087	20.613238937610717
51	17.962577053216318	34.019326961830345	25.339377825544958	22.67871815940838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	281.5
2	344.0
3	2328.5
4	4313.0
5	3359.5
6	2406.0
7	2514.0
8	2622.0
9	2772.0
10	2922.0
11	2888.0
12	2854.0
13	2743.0
14	2632.0
15	2495.5
16	2359.0
17	2188.0
18	2017.0
19	1909.0
20	1801.0
21	1697.0
22	1593.0
23	1585.0
24	1577.0
25	1668.5
26	2186.5
27	2613.0
28	3229.5
29	3846.0
30	4281.5
31	4717.0
32	5189.0
33	5661.0
34	6213.5
35	6766.0
36	8479.0
37	10192.0
38	14474.0
39	18756.0
40	23351.0
41	27946.0
42	30148.0
43	32350.0
44	31660.5
45	30971.0
46	29833.0
47	28695.0
48	26310.0
49	23925.0
50	22637.0
51	21349.0
52	19211.0
53	17073.0
54	15280.5
55	13488.0
56	12444.5
57	11401.0
58	10020.0
59	8639.0
60	7641.0
61	6643.0
62	5778.5
63	4914.0
64	4090.0
65	3266.0
66	2787.0
67	2308.0
68	2024.0
69	1740.0
70	1593.0
71	1446.0
72	1204.5
73	963.0
74	754.5
75	398.0
76	250.0
77	175.0
78	100.0
79	69.5
80	39.0
81	30.5
82	22.0
83	18.0
84	14.0
85	12.0
86	10.0
87	5.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.820394729963734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.15801387754792	22.642760897838123
2	7.229145333720926	4.600685161585506
3	3.7898088665959673	3.6177964225860046
4	2.556318115681659	3.2537220598538994
5	1.9374575044763314	3.082533128248367
6	1.5135749631298057	2.889753166810843
7	1.3006346954912167	2.897069658701169
8	1.120685233816978	2.8528517206478354
9	0.9397360567269629	2.6912495040328404
>10	8.342656801373824	47.96181991354509
>50	0.09397360567154886	1.8291589418439576
>100	0.014995788139076947	0.6177785504453853
>500	9.997192092717963E-4	0.3041175562439693
>1k	0.0019994384185435927	0.7587033176170155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1276	0.3986615469692662	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1109	0.3464856235022854	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	956	0.2986837295475066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01093507378050495	0.0
2	0.0	0.0	0.0	0.03499223609761584	0.0
3	0.0	0.0	0.0	0.0440527258014628	0.0
4	0.0	0.0	0.0	0.07154662559244668	0.0
5	0.0	0.0	0.0	0.12559713313608542	0.0
6	0.0	0.0	0.0	0.18589625426858417	0.0
7	0.0	0.0	0.0	0.222138213083972	0.0
8	0.0	0.0	0.0	0.5030133939032277	0.0
9	0.0	0.0	0.0	0.6204873293737951	0.0
10	0.0	0.0	0.0	0.885740976220901	0.0
11	0.0	0.0	0.0	1.0238353365347064	0.0
12	0.0	0.0	0.0	1.2222288179810106	0.0
13	0.0	0.0	0.0	1.2822155084340663	0.0
14	0.0	0.0	0.0	1.30346079463619	0.0
15	0.0	0.0	0.0	1.3384530307338058	0.0
16	0.0	0.0	0.0	1.4221844528245295	0.0
17	0.0	0.0	0.0	1.5137266419013282	0.0
18	0.0	0.0	0.0	1.6761905952116873	0.0
19	0.0	0.0	0.0	1.745862636727476	0.0
20	0.0	0.0	0.0	1.8152222475638218	0.0
21	0.0	0.0	0.0	1.8942672094629005	0.0
22	0.0	0.0	0.0	1.9698754338881062	0.0
23	0.0	0.0	0.0	2.05673116277326	0.0
24	0.0	0.0	0.0	2.1195297293413025	0.0
25	0.0	0.0	0.0	2.1682689153344104	0.0
26	0.0	0.0	0.0	2.228255605787466	0.0
27	0.0	0.0	0.0	2.288242296240522	0.0
28	0.0	0.0	0.0	2.3513532934880073	0.0
29	0.0	0.0	0.0	2.411652414620506	0.0
30	0.0	0.0	0.0	2.4981957128262167	0.0
31	0.0	0.0	0.0	2.571616922495321	0.0
32	0.0	0.0	0.0	2.648787300317742	0.0
33	0.0	0.0	0.0	2.7359554598823386	0.0
34	0.0	0.0	0.0	2.8096891002308864	0.0
35	0.0	0.0	0.0	2.918102545997607	0.0
36	0.0	0.0	0.0	3.0015215374088875	0.0
37	0.0	0.0	0.0	3.08306594474351	0.0
38	0.0	0.0	0.0	3.160861183924817	0.0
39	0.0	0.0	0.0	3.2464671900921984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGGC	25	3.8844788E-5	45.0	4
GTGCACT	20	7.0256507E-4	45.0	39
CTCACGA	80	0.0	45.0	24
CATATAG	20	7.0256507E-4	45.0	2
AGTTTCG	20	7.0256507E-4	45.0	31
CTCAACT	20	7.0256507E-4	45.0	23
ATAGGCC	35	1.2082637E-7	45.0	5
ACGTTAG	25	3.8844788E-5	45.0	2
TATGGGT	20	7.0256507E-4	45.0	4
CCGTGAG	20	7.0256507E-4	45.0	24
TGTATGA	20	7.0256507E-4	45.0	1
GTGATAC	25	3.8844788E-5	45.0	9
AAGGTAC	20	7.0256507E-4	45.0	6
CGATTCT	35	1.2082637E-7	45.0	10
AGGAACG	20	7.0256507E-4	45.0	7
GGATAGT	25	3.8844788E-5	45.0	8
GTCAGAC	20	7.0256507E-4	45.0	44
GCCGATG	20	7.0256507E-4	45.0	9
TACTGAC	45	3.8380676E-10	45.0	20
GTGTATG	20	7.0256507E-4	45.0	1
>>END_MODULE