FastQCFastQC Report
Thu 26 May 2016
SRR1545734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545734_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1188596
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.35949977957186463No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24550.20654621082352626No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.15589821941181026No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.1457181414038075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.856591E-444.020
ACACGTC207.856591E-444.040
CTCGTTA207.856591E-444.01
CGTATCA207.856591E-444.033
CGTATAT254.4429315E-544.014
CAACGGC254.4429315E-544.042
CGCATCG302.5277895E-644.021
GTGATCG254.4429315E-544.019
GTCTAAC302.5277895E-644.033
CGATTCG351.446133E-744.010
TACGCAA207.856591E-444.01
TCTACCG207.856591E-444.018
TACGACG207.856591E-444.01
CTCGACA302.5277895E-644.01
ACGTAGA254.4429315E-544.01
CGCAAAC254.4429315E-544.029
TATACGA207.856591E-444.01
TAAGACG254.4429315E-544.01
ACCCATC207.856591E-444.024
ACGACTC207.856591E-444.016
Produced by FastQC (version 0.11.5)