##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545734_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1188596
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557756378113336	31.0	31.0	33.0	30.0	34.0
2	31.735238045559637	31.0	31.0	34.0	30.0	34.0
3	31.785898656902766	31.0	31.0	34.0	30.0	34.0
4	35.68112630363891	37.0	35.0	37.0	35.0	37.0
5	27.142533712043452	35.0	26.0	37.0	0.0	37.0
6	31.372315740588054	35.0	26.0	37.0	17.0	37.0
7	14.968582260078277	0.0	0.0	32.0	0.0	37.0
8	25.08960824367573	17.0	17.0	35.0	17.0	37.0
9	33.22484006340254	32.0	32.0	37.0	28.0	39.0
10	36.11124553675092	37.0	35.0	39.0	32.0	39.0
11	37.0808954430269	39.0	37.0	39.0	34.0	39.0
12	36.95056436333287	39.0	35.0	39.0	33.0	39.0
13	36.38306792215353	39.0	35.0	39.0	32.0	39.0
14	37.71569229578427	40.0	36.0	41.0	33.0	41.0
15	38.024111640961266	40.0	36.0	41.0	33.0	41.0
16	38.093155285732074	40.0	36.0	41.0	33.0	41.0
17	38.128880628910075	40.0	36.0	41.0	34.0	41.0
18	38.103618891532534	40.0	36.0	41.0	34.0	41.0
19	38.026049221097836	40.0	36.0	41.0	34.0	41.0
20	38.04507502969891	40.0	36.0	41.0	34.0	41.0
21	37.90104880043345	40.0	35.0	41.0	34.0	41.0
22	37.809414637101256	40.0	35.0	41.0	33.0	41.0
23	37.72416700039374	39.0	35.0	41.0	33.0	41.0
24	37.58735011728123	39.0	35.0	41.0	33.0	41.0
25	37.3994948662119	39.0	35.0	41.0	33.0	41.0
26	37.152549730943065	39.0	35.0	40.0	33.0	41.0
27	36.945575283780194	39.0	35.0	40.0	32.0	41.0
28	36.99773598430417	39.0	35.0	40.0	32.0	41.0
29	36.95915096466756	38.0	35.0	40.0	33.0	41.0
30	36.77291443013438	38.0	35.0	40.0	32.0	41.0
31	36.56162817307142	38.0	35.0	40.0	31.0	41.0
32	36.32034013239149	38.0	35.0	40.0	31.0	41.0
33	36.14051284035955	38.0	35.0	40.0	31.0	41.0
34	35.91394721166822	38.0	35.0	40.0	30.0	41.0
35	35.761309982534016	38.0	35.0	40.0	30.0	41.0
36	35.57929691838101	38.0	35.0	40.0	29.0	41.0
37	35.42798309938785	38.0	34.0	40.0	29.0	41.0
38	35.249136796691225	38.0	34.0	40.0	28.0	41.0
39	35.22533560604276	38.0	34.0	40.0	28.0	41.0
40	34.98292859811071	37.0	34.0	40.0	27.0	41.0
41	34.81642038169403	37.0	34.0	40.0	26.0	40.0
42	34.808764289969005	37.0	34.0	40.0	26.0	41.0
43	34.67401202763597	37.0	34.0	40.0	26.0	41.0
44	34.45432173757946	37.0	34.0	40.0	25.0	40.0
45	34.16576532312072	36.0	33.0	39.0	24.0	40.0
46	34.16566941164197	36.0	33.0	39.0	24.0	40.0
47	34.033782715068874	36.0	33.0	39.0	24.0	40.0
48	33.98998061578535	36.0	33.0	39.0	24.0	40.0
49	33.88509552446752	36.0	33.0	39.0	24.0	40.0
50	33.94511171163288	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	2.0
13	4.0
14	5.0
15	20.0
16	51.0
17	147.0
18	356.0
19	840.0
20	1551.0
21	2658.0
22	4199.0
23	6387.0
24	9964.0
25	14039.0
26	18220.0
27	20021.0
28	20971.0
29	23930.0
30	29248.0
31	39304.0
32	55673.0
33	106301.0
34	103100.0
35	125248.0
36	155217.0
37	289545.0
38	130687.0
39	30904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.49775701752319	23.366980874914606	28.05292967501153	15.082332432550674
2	32.8066054403683	24.687278099539288	30.050580685110837	12.455535774981573
3	31.002544178173242	23.639318994847702	32.719864445110034	12.638272381869028
4	27.422101370019753	26.51413937115723	31.462582744683644	14.601176514139372
5	16.85349774019095	48.56057062281886	23.713355925814994	10.872575711175202
6	20.586557585588373	40.45882705309457	28.75451372880272	10.200101632514327
7	39.14509219280563	55.87743859141373	3.982177291527146	0.9952919242534889
8	87.80426654641275	2.9301798087827993	7.60157362131456	1.663980023489899
9	83.72062500630997	4.769324480311225	9.02569081504565	2.4843596983331597
10	53.651198556952906	21.098422003775884	13.866191708536796	11.384187730734412
11	48.52018684229124	18.97903072196104	19.077802718501495	13.42297971724623
12	42.37335478160788	19.98870936802749	23.665400186438454	13.972535663926177
13	22.150082955015833	38.740665457396794	24.50496215703233	14.604289430555042
14	16.101602226492435	41.85021655802308	27.33838915830105	14.709792057183432
15	14.538245122817173	21.502175676175924	48.308424393149565	15.651154807857337
16	15.824720931249978	16.158307784983293	48.45675065371245	19.560220630054282
17	16.56113599574624	16.504346304379283	30.023237500378595	36.91128019949588
18	21.22647224119886	22.622657320065017	34.91951849072351	21.231351948012612
19	29.547634351789842	22.98770986945943	26.16793258600904	21.296723192741688
20	31.11536636502226	22.227485201027093	24.696869247414597	21.96027918653605
21	22.988046400963828	26.94759194882029	28.57169298903917	21.492668661176715
22	22.629051418648558	23.224712181430863	25.869092610104694	28.277143789815884
23	20.03851603067821	30.019283255201934	25.214538834052952	24.727661880066904
24	20.929819720073095	23.540294599678948	37.96437140962951	17.565514270618444
25	18.892457992454965	22.960114286098893	36.78583808123197	21.361589640214167
26	17.07939451251729	32.27009008948373	28.02533409165099	22.625181306347994
27	18.696596656896038	30.92202901574631	29.79077836371652	20.590595963641135
28	15.677572530952485	27.48427556545706	39.46328273021279	17.374869173377665
29	16.56231385601163	24.7172294034306	37.30392833225083	21.41652840830694
30	19.27164486503404	27.9615613715678	32.652137479850175	20.114656283547987
31	30.59130267980037	24.694008729627225	24.74474085391504	19.969947736657367
32	29.719938482040998	25.369679857579868	27.73061662667551	17.17976503370363
33	28.19696515889335	24.981827298762575	25.70814641812693	21.113061124217143
34	19.824902658262353	26.549306913366692	28.94095218223854	24.68483824613241
35	20.567964219970452	23.48485103432958	32.23811959656604	23.709065149133938
36	30.9544201730445	23.555101985872405	26.16271634769089	19.327761493392202
37	21.014709792057186	31.057398813389913	27.73827271840053	20.189618676152367
38	21.942022352422523	32.545709391584694	22.889947467432165	22.62232078856062
39	21.781917489205753	29.443309585426842	25.497814227878944	23.276958697488464
40	24.246337695903403	24.37379900319368	25.697629808614536	25.682233492288383
41	18.749011438705836	23.165061972276536	27.222874719416858	30.86305186960077
42	22.317928042833728	27.090449572436725	22.586311917590166	28.00531046713938
43	22.787389491467245	25.880787079882484	24.58362639618508	26.748197032465193
44	20.870001245166566	29.676273519345514	27.59406896876651	21.85965626672141
45	19.07511046646632	35.33530316440574	23.365214084516523	22.224372284611423
46	22.88801241128188	30.668115995678935	26.822402229184682	19.621469363854498
47	22.97929658184951	27.10618242026727	26.3812935597966	23.533227438086616
48	23.6710370891371	24.109116974985614	29.560843213337417	22.65900272253987
49	21.52480741984661	23.472735900171294	31.926323157742413	23.076133522239683
50	20.506715486170236	30.362797788314953	26.96340892952694	22.167077795987872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	627.0
1	1241.5
2	1856.0
3	7857.0
4	13858.0
5	10299.5
6	6741.0
7	6891.0
8	7041.0
9	7264.5
10	7488.0
11	7413.0
12	7338.0
13	7018.0
14	6698.0
15	6309.5
16	5921.0
17	5499.5
18	5078.0
19	4848.0
20	4618.0
21	4502.0
22	4386.0
23	4864.5
24	5343.0
25	6346.5
26	7350.0
27	8759.0
28	10168.0
29	11947.5
30	13727.0
31	15706.0
32	17685.0
33	19669.0
34	21653.0
35	23138.5
36	24624.0
37	31107.0
38	37590.0
39	53926.0
40	70262.0
41	88211.5
42	106161.0
43	110471.0
44	114781.0
45	112202.5
46	109624.0
47	103619.5
48	97615.0
49	94303.5
50	90992.0
51	84091.5
52	77191.0
53	69170.0
54	61149.0
55	57538.5
56	53928.0
57	51272.0
58	48616.0
59	45596.5
60	42577.0
61	37928.0
62	33279.0
63	27950.5
64	22622.0
65	19053.5
66	15485.0
67	13798.0
68	12111.0
69	10756.5
70	9402.0
71	7947.5
72	6493.0
73	5085.5
74	3678.0
75	2611.0
76	1544.0
77	1117.5
78	691.0
79	500.0
80	309.0
81	227.5
82	146.0
83	110.0
84	74.0
85	52.0
86	30.0
87	23.5
88	17.0
89	20.0
90	23.0
91	13.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1188596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.243753820611023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45249172963102	14.869541600851177
2	6.002921274672665	2.430433209779639
3	2.6892978803148324	1.6332445271815352
4	1.7154409980678873	1.3890786103467834
5	1.296460373648997	1.312261232116384
6	1.090004781413011	1.3239473074928354
7	0.9111469950831468	1.2911524823996898
8	0.7858232594542383	1.2726410088721396
9	0.7131005611972205	1.299224898919449
>10	9.515202940736973	43.90014088138506
>50	1.6242391841917156	22.06108902222339
>100	0.1989412957916846	5.912176293703409
>500	0.003136461870589622	0.36962440007427727
>1k	0.0017922639260512126	0.9354445246542383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4273	0.35949977957186463	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2455	0.20654621082352626	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1853	0.15589821941181026	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.1457181414038075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006646497211836486	0.0
2	0.0	0.0	0.0	0.021958680661890162	0.0
3	0.0	0.0	0.0	0.029867171015214588	0.0
4	0.0	0.0	0.0	0.04795573937654173	0.0
5	0.0	0.0	0.0	0.07790704326785552	0.0
6	0.0	0.0	0.0	0.14546574277550994	0.0
7	0.0	0.0	0.0	0.19527240542623397	0.0
8	0.0	0.0	0.0	0.5127898798245998	0.0
9	0.0	0.0	0.0	0.664397322555351	0.0
10	0.0	0.0	0.0	0.9030822920487701	0.0
11	0.0	0.0	0.0	1.0604949032303659	0.0
12	0.0	0.0	0.0	1.236500880029884	0.0
13	0.0	0.0	0.0	1.2990116069715867	0.0
14	0.0	0.0	0.0	1.3196241616158897	0.0
15	0.0	0.0	0.0	1.3475562764808227	0.0
16	0.0	0.0	0.0	1.4187326896607426	0.0
17	0.0	0.0	0.0	1.5048847547863193	0.0
18	0.0	0.0	0.0	1.6668405412772718	0.0
19	0.0	0.0	0.0	1.7239667641486258	0.0
20	0.0	0.0	0.0	1.7948066458241487	0.0
21	0.0	0.0	0.0	1.8689277096675405	0.0
22	0.0	0.0	0.0	1.9406089201040555	0.0
23	0.0	0.0	0.0	2.027181649610128	0.0
24	0.0	0.0	0.0	2.09499274774608	0.0
25	0.0	0.0	0.0	2.1481647254407723	0.0
26	0.0	0.0	0.0	2.20697360583411	0.0
27	0.0	0.0	0.0	2.2646046259620594	0.0
28	0.0	0.0	0.0	2.320552988568025	0.0
29	0.0	0.0	0.0	2.3817175894921405	0.0
30	0.0	0.0	0.0	2.4624851505473684	0.0
31	0.0	0.0	0.0	2.540728725319621	0.0
32	0.0	0.0	0.0	2.613756061773723	0.0
33	0.0	0.0	0.0	2.6865309995995275	0.0
34	0.0	0.0	0.0	2.763176049725895	0.0
35	0.0	0.0	0.0	2.873979047548536	0.0
36	0.0	0.0	0.0	2.9570181962584425	0.0
37	0.0	0.0	0.0	3.0411510723576387	0.0
38	0.0	0.0	0.0	3.1258728785895293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.856591E-4	44.0	20
ACACGTC	20	7.856591E-4	44.0	40
CTCGTTA	20	7.856591E-4	44.0	1
CGTATCA	20	7.856591E-4	44.0	33
CGTATAT	25	4.4429315E-5	44.0	14
CAACGGC	25	4.4429315E-5	44.0	42
CGCATCG	30	2.5277895E-6	44.0	21
GTGATCG	25	4.4429315E-5	44.0	19
GTCTAAC	30	2.5277895E-6	44.0	33
CGATTCG	35	1.446133E-7	44.0	10
TACGCAA	20	7.856591E-4	44.0	1
TCTACCG	20	7.856591E-4	44.0	18
TACGACG	20	7.856591E-4	44.0	1
CTCGACA	30	2.5277895E-6	44.0	1
ACGTAGA	25	4.4429315E-5	44.0	1
CGCAAAC	25	4.4429315E-5	44.0	29
TATACGA	20	7.856591E-4	44.0	1
TAAGACG	25	4.4429315E-5	44.0	1
ACCCATC	20	7.856591E-4	44.0	24
ACGACTC	20	7.856591E-4	44.0	16
>>END_MODULE