##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545731_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	391071
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03293263883029	31.0	31.0	33.0	30.0	33.0
2	31.18553919876442	31.0	31.0	33.0	30.0	34.0
3	31.10610860943409	31.0	31.0	34.0	28.0	34.0
4	35.072280992454054	35.0	35.0	37.0	32.0	37.0
5	32.83653607656922	35.0	35.0	37.0	28.0	37.0
6	34.024261579099445	35.0	35.0	37.0	28.0	37.0
7	15.24677360377016	0.0	0.0	35.0	0.0	37.0
8	24.90069066742356	17.0	17.0	35.0	17.0	37.0
9	32.76631609094001	32.0	32.0	37.0	27.0	39.0
10	35.57256610692176	37.0	35.0	37.0	32.0	39.0
11	36.52543911463647	37.0	35.0	39.0	32.0	39.0
12	36.24950967982796	37.0	35.0	39.0	32.0	39.0
13	35.775818201809905	37.0	35.0	39.0	31.0	39.0
14	36.81475997964564	39.0	35.0	40.0	31.0	41.0
15	37.26064065093039	39.0	35.0	41.0	32.0	41.0
16	37.37192223407003	39.0	35.0	41.0	32.0	41.0
17	37.34534649718338	39.0	35.0	41.0	32.0	41.0
18	37.246597165220635	39.0	35.0	40.0	32.0	41.0
19	37.14852801665171	39.0	35.0	40.0	32.0	41.0
20	37.22971532023597	39.0	35.0	40.0	32.0	41.0
21	37.03472003804936	39.0	35.0	40.0	32.0	41.0
22	37.08922932152985	39.0	35.0	40.0	32.0	41.0
23	37.0789600865316	39.0	35.0	40.0	32.0	41.0
24	36.888465265898006	38.0	35.0	40.0	32.0	41.0
25	36.62020451529262	38.0	35.0	40.0	31.0	41.0
26	36.50335361098113	38.0	35.0	40.0	31.0	41.0
27	36.391338145758695	38.0	35.0	40.0	31.0	41.0
28	36.3266465680145	38.0	35.0	40.0	31.0	41.0
29	36.37629228452123	38.0	35.0	40.0	31.0	41.0
30	36.11781492363279	38.0	35.0	40.0	30.0	41.0
31	35.94661838898819	38.0	34.0	40.0	30.0	41.0
32	35.781062773767424	38.0	34.0	40.0	29.0	41.0
33	35.542118950267344	38.0	34.0	40.0	29.0	41.0
34	35.3633534575563	38.0	34.0	40.0	27.0	41.0
35	35.17041662511411	38.0	34.0	40.0	26.0	41.0
36	34.93309910476614	38.0	34.0	40.0	25.0	41.0
37	34.731391486456424	38.0	34.0	40.0	24.0	41.0
38	34.67339434527234	38.0	33.0	40.0	24.0	41.0
39	34.59009233617425	37.0	33.0	40.0	23.0	41.0
40	34.420297081604105	37.0	33.0	40.0	23.0	41.0
41	34.32893771207786	37.0	33.0	40.0	23.0	41.0
42	34.28196414461824	37.0	33.0	40.0	23.0	41.0
43	34.184255544389636	37.0	33.0	40.0	23.0	41.0
44	33.9066435506596	36.0	33.0	40.0	22.0	41.0
45	33.83999324930767	36.0	33.0	40.0	22.0	41.0
46	33.83823397797331	36.0	33.0	40.0	22.0	41.0
47	33.8026368613372	36.0	33.0	40.0	22.0	41.0
48	33.79884215398227	36.0	33.0	40.0	22.0	41.0
49	33.72131403249026	36.0	33.0	40.0	22.0	41.0
50	33.550414119175294	36.0	33.0	40.0	21.0	41.0
51	33.35064988199074	35.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	6.0
15	15.0
16	50.0
17	130.0
18	287.0
19	514.0
20	900.0
21	1482.0
22	2359.0
23	3376.0
24	4865.0
25	6416.0
26	7578.0
27	7940.0
28	8321.0
29	9437.0
30	11380.0
31	14764.0
32	19715.0
33	30329.0
34	39482.0
35	39606.0
36	43224.0
37	65614.0
38	62007.0
39	11271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.04339365486063	24.66125077032048	29.08730128288725	16.208054291931646
2	32.783561041345436	24.563058881891013	30.255119914286666	12.39826016247689
3	28.846680014626504	23.00502977720158	34.77143536595656	13.376854842215351
4	26.81252253427127	27.231628016395998	31.480217147269933	14.475632302062797
5	20.553812479063904	36.994049673844394	29.874370638579695	12.57776720851201
6	19.850103945319393	40.45505803294031	29.581840637633576	10.112997384106723
7	37.495237437703125	57.1195511812433	4.776370531182318	0.608840849871251
8	87.77715555487366	2.9214643888194214	7.865835104111529	1.4355449521953814
9	83.35570778707702	4.6523521304315585	9.601837006579368	2.3901030759120467
10	54.000424475351025	21.383329369858668	14.500180274170162	10.116065880620143
11	49.111030989257706	18.173170600734906	20.5333558356411	12.182442574366291
12	44.19120824607297	19.819930396270756	23.96981622262965	12.019045135026632
13	20.409081726847557	43.10675043662148	24.010985217518048	12.473182619012915
14	14.251120640497506	45.129145347008595	26.972595769054724	13.647138243439173
15	12.867740129030278	21.868407527021947	51.06285047983614	14.201001864111632
16	13.787010542842603	16.08787151182266	50.15610976012028	19.96900818521445
17	14.039650089114254	17.17386356953085	30.471960334568404	38.314526006786494
18	20.772187147602352	21.993704467986632	36.280112818388474	20.953995566022538
19	29.101109517197642	22.834472512663943	26.704613740215972	21.359804229922442
20	31.536728624725434	21.895001163471594	25.17675818457518	21.391512027227794
21	21.05193174640922	27.753783839763113	29.30618736751127	21.8880970463164
22	21.642617325242732	24.10789856573359	25.739571586745118	28.50991252227856
23	18.2023213176124	29.3381508728594	25.703516752712424	26.75601105681577
24	18.42862293547719	22.70227145454406	42.437306780610164	16.431798829368578
25	16.117277936742944	24.267204676388687	38.620608534000226	20.99490885286815
26	15.579523922765942	34.03192770622214	28.65771177100833	21.73083660000358
27	16.56399988748846	33.816877242239904	29.92960357582127	19.68951929445037
28	13.714900874777214	28.027135737500352	41.04753356807332	17.210429819649118
29	13.992344101199015	22.96565073861268	39.7135047088636	23.328500451324697
30	17.848165678355084	30.27966788639403	33.515397459796304	18.356768975454585
31	29.208762603210157	25.91933434082302	25.781251997719085	19.090651058247733
32	30.70414323741725	24.622383147817146	26.983846922937264	17.689626691828337
33	27.9184598193167	27.208102876459773	25.003132423524114	19.870304880699415
34	18.43066859981947	27.275865507797814	28.695812269383307	25.597653622999406
35	19.883601698924235	24.74537871639677	31.731578153327654	23.63944143135134
36	32.11488450946248	23.99206282235195	25.935443947518483	17.95760872066709
37	20.21730069475875	32.643433033899214	28.99754775986969	18.14171851147234
38	20.21551073845926	32.50407215058135	25.169854067419983	22.110563043539408
39	20.445392268923037	32.092637909740176	27.665564565002263	19.79640525633453
40	24.328574606657103	25.856941578383463	25.898366281314644	23.916117533644783
41	17.519069427290695	23.55659202548898	27.95681602573446	30.96752252148587
42	22.26552211746716	25.188265046500508	24.139606363038936	28.406606472993396
43	21.993960176029418	25.902713318041993	25.98326135151929	26.1200651544093
44	19.193445691447334	29.895594406130854	29.291356301029737	21.619603601392072
45	17.123233377059407	38.25903736150213	22.564956235568477	22.052773025869982
46	22.27063627832286	32.599451250540184	25.639078325930587	19.49083414520637
47	21.35801427362295	27.742532685880565	27.11911647757057	23.780336562925914
48	22.299019871072005	23.900007926949325	31.163650590302016	22.637321611676654
49	20.78599538191275	24.03553318962541	32.625789178947045	22.552682249514795
50	20.026031078755523	32.64292161781365	26.968760148412947	20.362287155017885
51	18.393079517530065	33.68160768760659	24.44441035003874	23.480902444824597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	330.0
1	431.0
2	532.0
3	2316.5
4	4101.0
5	3160.0
6	2219.0
7	2285.0
8	2351.0
9	2466.5
10	2582.0
11	2582.5
12	2583.0
13	2528.0
14	2473.0
15	2351.5
16	2230.0
17	2037.0
18	1844.0
19	1737.5
20	1631.0
21	1650.0
22	1669.0
23	1764.0
24	1859.0
25	2147.0
26	2826.0
27	3217.0
28	3956.5
29	4696.0
30	5681.0
31	6666.0
32	7646.5
33	8627.0
34	9159.0
35	9691.0
36	11131.5
37	12572.0
38	16872.0
39	21172.0
40	27387.0
41	33602.0
42	37405.0
43	41208.0
44	39796.5
45	38385.0
46	36536.0
47	34687.0
48	32043.0
49	29399.0
50	27235.0
51	25071.0
52	22723.0
53	20375.0
54	18574.0
55	16773.0
56	15282.5
57	13792.0
58	12399.5
59	11007.0
60	9942.5
61	8878.0
62	7859.5
63	6841.0
64	5829.0
65	4817.0
66	4161.0
67	3505.0
68	3073.5
69	2642.0
70	2231.0
71	1820.0
72	1536.0
73	1252.0
74	962.0
75	550.5
76	429.0
77	322.5
78	216.0
79	187.0
80	158.0
81	100.5
82	43.0
83	29.0
84	15.0
85	8.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.975176071078565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92703888398633	22.279526937027903
2	7.320836558270474	4.535284027600334
3	3.672359940889073	3.412559872962441
4	2.393777512654339	2.9659071971782653
5	1.7251778943489928	2.671884451569631
6	1.3942040651056926	2.5911429837397386
7	1.1853255900653936	2.570096819768146
8	0.9840279033772301	2.438435005277119
9	0.8297774934077349	2.3132253562310536
>10	8.4264288134513	50.37934542775345
>50	0.12702329265667306	2.49385237631822
>100	0.01154757205969755	0.5378095740344078
>500	8.248265756926822E-4	0.2342856909214023
>1k	0.0016496531513853644	0.5766442796178899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.30531540308537325	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2718176494805291	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	917	0.2344842752339089	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006648409112411813	0.0
2	0.0	0.0	0.0	0.033753461647629206	0.0
3	0.0	0.0	0.0	0.04500461553017227	0.0
4	0.0	0.0	0.0	0.07313250023652994	0.0
5	0.0	0.0	0.0	0.12964397769203034	0.0
6	0.0	0.0	0.0	0.20533355835641098	0.0
7	0.0	0.0	0.0	0.2541737945283593	0.0
8	0.0	0.0	0.0	0.630576033507982	0.0
9	0.0	0.0	0.0	0.8128958680137366	0.0
10	0.0	0.0	0.0	1.1026130804892207	0.0
11	0.0	0.0	0.0	1.328147574225652	0.0
12	0.0	0.0	0.0	1.5498464473203075	0.0
13	0.0	0.0	0.0	1.6401113864234371	0.0
14	0.0	0.0	0.0	1.6595451976751026	0.0
15	0.0	0.0	0.0	1.7048055212480597	0.0
16	0.0	0.0	0.0	1.8037645338058819	0.0
17	0.0	0.0	0.0	1.9282943506422108	0.0
18	0.0	0.0	0.0	2.149226099608511	0.0
19	0.0	0.0	0.0	2.226705636572387	0.0
20	0.0	0.0	0.0	2.319016240017797	0.0
21	0.0	0.0	0.0	2.4225779973457504	0.0
22	0.0	0.0	0.0	2.5215370099035725	0.0
23	0.0	0.0	0.0	2.6358385050284987	0.0
24	0.0	0.0	0.0	2.721756407404282	0.0
25	0.0	0.0	0.0	2.7895190387423257	0.0
26	0.0	0.0	0.0	2.86086158267936	0.0
27	0.0	0.0	0.0	2.9357840392153856	0.0
28	0.0	0.0	0.0	3.015309240521542	0.0
29	0.0	0.0	0.0	3.099181478555045	0.0
30	0.0	0.0	0.0	3.2106701852093353	0.0
31	2.557080427850697E-4	0.0	0.0	3.311674862109438	0.0
32	2.557080427850697E-4	0.0	0.0	3.3970813483996514	0.0
33	2.557080427850697E-4	0.0	0.0	3.490926200101772	0.0
34	2.557080427850697E-4	0.0	0.0	3.583492511589967	0.0
35	2.557080427850697E-4	0.0	0.0	3.7123693651536422	0.0
36	2.557080427850697E-4	0.0	0.0	3.8200224511661567	0.0
37	2.557080427850697E-4	0.0	0.0	3.9138673028682773	0.0
38	2.557080427850697E-4	0.0	0.0	4.0102692349982485	0.0
39	2.557080427850697E-4	0.0	0.0	4.105904042999865	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCAC	60	0.0	45.000004	22
AGGCGAT	260	0.0	45.000004	7
CTACGGG	30	2.1618143E-6	45.000004	3
TCGTAAG	30	2.1618143E-6	45.000004	2
CGGTCTA	60	0.0	45.000004	31
TCACGAA	20	7.027572E-4	45.0	34
CGTATGA	45	3.8380676E-10	45.0	15
CGCGGAT	20	7.027572E-4	45.0	39
ATTCGGT	25	3.88607E-5	45.0	38
GTTAGCG	25	3.88607E-5	45.0	1
TAACGTG	20	7.027572E-4	45.0	32
CACGAGG	35	1.2091732E-7	45.0	3
GAATAAC	20	7.027572E-4	45.0	9
CGCGAAG	20	7.027572E-4	45.0	2
GCGTTAG	20	7.027572E-4	45.0	2
TCGATAG	20	7.027572E-4	45.0	2
CTCGGTA	25	3.88607E-5	45.0	21
AATGACC	20	7.027572E-4	45.0	9
CGCTATG	25	3.88607E-5	45.0	24
GTATGAC	45	3.8380676E-10	45.0	16
>>END_MODULE