FastQCFastQC Report
Thu 26 May 2016
SRR1545730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545730_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences894066
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27930.3123930448087725No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.3042280994915364No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.1290732451519239No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.12739551666208088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAT207.8555424E-444.07
TCGCCTG207.8555424E-444.017
TCTGCGA207.8555424E-444.01
AACCGTA254.442045E-544.016
CGGGTAT750.044.06
AGCGTAA408.309144E-944.01
CTCACGT207.8555424E-444.044
CTCACGA1500.044.024
CTCGTTA254.442045E-544.01
ACGCCCG254.442045E-544.010
GGTGTCG254.442045E-544.08
CGAGTGA207.8555424E-444.041
GGTCGCT254.442045E-544.08
CCTAGTC254.442045E-544.026
CGAAACA207.8555424E-444.038
ACCGCTC302.5270838E-644.018
GTTTACG254.442045E-544.01
CGTTGTA302.5270838E-644.01
TAGCGTG207.8555424E-444.010
ATTACTA408.309144E-944.01
Produced by FastQC (version 0.11.5)