##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545730_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	894066
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584689497195956	31.0	31.0	33.0	30.0	34.0
2	31.76518176510459	31.0	31.0	34.0	30.0	34.0
3	31.80462180644382	31.0	31.0	34.0	30.0	34.0
4	35.6750519536589	37.0	35.0	37.0	35.0	37.0
5	27.209780933398655	35.0	26.0	37.0	0.0	37.0
6	31.39151024644713	35.0	28.0	37.0	17.0	37.0
7	14.87688716492966	0.0	0.0	32.0	0.0	37.0
8	25.065904530538013	17.0	17.0	35.0	17.0	37.0
9	33.32155679782029	32.0	32.0	37.0	28.0	39.0
10	36.21840893177909	37.0	35.0	39.0	33.0	39.0
11	37.14873957850986	39.0	37.0	39.0	34.0	39.0
12	36.77370686280431	39.0	35.0	39.0	33.0	39.0
13	36.02109352105997	38.0	35.0	39.0	32.0	39.0
14	37.2770567273557	39.0	35.0	41.0	33.0	41.0
15	37.688809327275614	40.0	35.0	41.0	33.0	41.0
16	37.83723461131505	40.0	35.0	41.0	33.0	41.0
17	37.900462605668935	40.0	35.0	41.0	34.0	41.0
18	37.891950929797126	40.0	36.0	41.0	34.0	41.0
19	37.809139370024134	40.0	36.0	41.0	34.0	41.0
20	37.79887950106592	39.0	35.0	41.0	34.0	41.0
21	37.61511230714511	39.0	35.0	41.0	33.0	41.0
22	37.53673889847058	39.0	35.0	41.0	33.0	41.0
23	37.44379385861894	39.0	35.0	41.0	33.0	41.0
24	37.30900403325929	39.0	35.0	41.0	33.0	41.0
25	37.134735019562314	38.0	35.0	41.0	33.0	41.0
26	36.90648117700483	38.0	35.0	40.0	33.0	41.0
27	36.661301290956146	38.0	35.0	40.0	32.0	41.0
28	36.75988573550499	38.0	35.0	40.0	32.0	41.0
29	36.806375591958535	38.0	35.0	40.0	33.0	41.0
30	36.576605082846235	38.0	35.0	40.0	32.0	41.0
31	36.329498046005554	38.0	35.0	40.0	31.0	41.0
32	36.010612191941085	38.0	35.0	40.0	31.0	41.0
33	35.81488055691638	38.0	35.0	40.0	30.0	41.0
34	35.646715119465455	38.0	35.0	40.0	30.0	41.0
35	35.548796173884256	38.0	35.0	40.0	30.0	41.0
36	35.321139602669156	38.0	34.0	40.0	29.0	41.0
37	35.071019365460714	37.0	34.0	40.0	27.0	41.0
38	34.943501933861704	37.0	34.0	40.0	27.0	41.0
39	34.959651748304935	37.0	34.0	40.0	27.0	41.0
40	34.71940102855941	37.0	34.0	40.0	27.0	40.0
41	34.59844015989871	37.0	34.0	40.0	26.0	40.0
42	34.615199549026585	37.0	34.0	40.0	26.0	40.0
43	34.50445716535468	36.0	33.0	40.0	26.0	41.0
44	34.296721942227975	36.0	34.0	40.0	24.0	40.0
45	34.00467191460138	36.0	33.0	39.0	24.0	40.0
46	34.029001214675425	36.0	33.0	39.0	24.0	40.0
47	33.932172792612626	36.0	33.0	39.0	24.0	40.0
48	33.960382119440844	36.0	33.0	39.0	24.0	40.0
49	33.849531242659936	36.0	33.0	39.0	24.0	40.0
50	33.85649381589278	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	2.0
14	9.0
15	14.0
16	50.0
17	116.0
18	342.0
19	668.0
20	1324.0
21	2123.0
22	3531.0
23	5338.0
24	8072.0
25	11732.0
26	14803.0
27	15772.0
28	16323.0
29	18268.0
30	22258.0
31	30607.0
32	44765.0
33	94090.0
34	83168.0
35	93621.0
36	102665.0
37	205968.0
38	98674.0
39	19757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.095093650804305	24.08211474320688	30.210297673773525	15.612493932215296
2	31.86263653913693	24.39316560522378	31.61791187675183	12.126285978887465
3	29.20466721696161	23.279153887968	34.27297313621142	13.243205758858966
4	25.71700523227592	27.598745506483862	32.23240789829834	14.451841362941886
5	17.439540257654357	48.30728380231437	24.179646692749753	10.07352924728152
6	19.635463153726906	40.11594222350475	29.518849838826217	10.729744783942124
7	38.97195509056378	56.255690295794714	3.8436759702303855	0.928678643411113
8	88.98504137278455	2.5265472571376164	7.160880740348027	1.3275306297297962
9	85.77152022333921	3.6858576436191512	8.727208058465482	1.8154140745761498
10	62.37335946115835	17.563244771638782	12.233884299369398	7.829511467833471
11	59.11230267116745	15.080430303803075	17.66435587529332	8.14291114973615
12	52.42957455042468	20.160368473915796	18.69358637952903	8.716470596130486
13	19.710625390071872	51.19577301899413	19.175653698943925	9.917947891990076
14	11.527560605145482	51.33905103202672	25.75145459060069	11.381933772227107
15	9.3127353014207	20.810096793749008	58.802705840508416	11.074462064321873
16	10.908366943827414	13.782092149796549	56.55824066679641	18.75130023957963
17	11.801477743253853	14.60943599242114	29.752389644612364	43.83669661971264
18	20.091246060134264	21.1799800014764	37.727192399666244	21.00158153872309
19	29.94197296396463	22.94550961562122	25.76140911297376	21.351108307440388
20	34.16022978169397	20.692543950894006	24.829263164016975	20.31796310339505
21	20.123905841403207	29.374565188699712	28.40506181870242	22.096467151194656
22	22.30238036118139	24.325944617064067	24.252012714944982	29.119662306809563
23	17.247608118416313	32.549834128576634	23.933691696138766	26.268866056868283
24	17.839398881066945	21.48420810096794	46.18383877700304	14.492554240962077
25	14.004558947549734	24.26196723731805	41.262166327765506	20.471307487366705
26	13.822133936420803	38.04707929839631	28.224426384629325	19.90636038055356
27	15.26956622889138	37.5427541143495	29.2774806334208	17.910199023338322
28	11.61010484684576	28.811295810376414	44.16922240639953	15.4093769363783
29	11.90806942664188	23.115183890227343	42.43534593642975	22.541400746701026
30	16.52886923336756	32.10959817284183	33.70131511543891	17.66021747835171
31	31.861853599175006	26.353647269888352	23.780571009299088	18.003928121637554
32	33.511060704690706	25.38884153966262	25.76465272138746	15.335445034259216
33	29.068659360718335	28.082378705822613	24.328293436949842	18.520668496509206
34	17.730682074925117	26.64512463285708	29.4773540208441	26.1468392713737
35	18.9921102021551	24.828703921187024	32.95562072598667	23.223565150671202
36	34.09826567613577	23.250073260810723	25.39398657369814	17.25767448935537
37	19.56969619692506	34.15083450215084	29.129057586352687	17.150411714571405
38	19.532674321582526	34.48872902000523	24.089944142826145	21.8886525155861
39	19.81363791934824	33.40178465571893	26.716372169392415	20.06820525554042
40	24.42358841517293	25.35976091250534	24.01165014663347	26.20500052568826
41	14.957620578346564	23.11160473611568	28.28281133607586	33.6479633494619
42	21.710589598530756	24.84212574910577	22.841266752118973	30.606017900244503
43	22.703133773121895	26.639308507425625	24.152355642648303	26.505202076804174
44	19.5403918726358	32.378929519744624	27.010869443642864	21.069809163976707
45	15.598624710032594	41.6385367523203	19.980515979804622	22.782322557842484
46	21.311513915080095	35.2437068404346	25.596656175271175	17.848123069214132
47	21.366655258112935	27.304807475063363	27.229757087284383	24.098780179539318
48	23.07245773801934	21.99379016761626	32.43496565130538	22.498786443059014
49	20.84834900331743	22.437605277462737	33.65243729210148	23.061608427118355
50	17.890178130026193	35.52489413533229	25.629875199370066	20.955052535271445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	769.0
1	954.0
2	1139.0
3	4606.0
4	8073.0
5	6207.0
6	4341.0
7	4733.5
8	5126.0
9	5624.5
10	6123.0
11	6251.0
12	6379.0
13	6093.0
14	5807.0
15	5374.0
16	4941.0
17	4652.0
18	4363.0
19	3842.5
20	3322.0
21	3076.0
22	2830.0
23	3116.5
24	3403.0
25	3879.0
26	4355.0
27	5112.0
28	5869.0
29	8208.5
30	10548.0
31	12398.0
32	14248.0
33	15763.0
34	17278.0
35	19346.5
36	21415.0
37	27898.0
38	34381.0
39	51919.0
40	69457.0
41	87365.0
42	105273.0
43	106778.0
44	108283.0
45	101294.0
46	94305.0
47	87265.5
48	80226.0
49	74367.0
50	68508.0
51	61512.5
52	54517.0
53	46866.0
54	39215.0
55	33670.5
56	28126.0
57	25361.0
58	22596.0
59	20711.0
60	18826.0
61	16681.5
62	14537.0
63	12099.5
64	9662.0
65	7453.5
66	5245.0
67	4579.0
68	3913.0
69	3321.5
70	2730.0
71	2166.5
72	1603.0
73	1356.0
74	1109.0
75	876.5
76	644.0
77	432.5
78	221.0
79	201.5
80	182.0
81	139.5
82	97.0
83	65.5
84	34.0
85	24.0
86	14.0
87	11.0
88	8.0
89	13.5
90	19.0
91	11.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	894066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.247121824559116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74454251445263	14.578644373211668
2	5.995413967650986	2.307889260480837
3	2.3928856236853955	1.381684833339268
4	1.4076988350199324	1.0837660387967434
5	0.9957397596068032	0.9582562229354673
6	0.823755323738833	0.9512951441778269
7	0.7205582464085796	0.9708070645221633
8	0.5958400455722835	0.9174564756064478
9	0.5778255487521208	1.0009330857157315
>10	8.318291346010781	38.246063027747965
>50	2.0872359025051446	28.102688141359778
>100	0.3372285630298561	8.536679185475272
>500	5.96864713327179E-4	0.0670894986451024
>1k	0.002387458853308716	0.896747647985735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2793	0.3123930448087725	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2720	0.3042280994915364	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.1290732451519239	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1139	0.12739551666208088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011408553730932615	0.0
2	0.0	0.0	0.0	0.03758111817248391	0.0
3	0.0	0.0	0.0	0.05044370326128049	0.0
4	0.0	0.0	0.0	0.07001720230944919	0.0
5	0.0	0.0	0.0	0.10994714036771334	0.0
6	0.0	0.0	0.0	0.19137289640809516	0.0
7	0.0	0.0	0.0	0.2464023908749466	0.0
8	0.0	0.0	0.0	0.596376553856203	0.0
9	0.0	0.0	0.0	0.75117496918572	0.0
10	0.0	0.0	0.0	0.9989195428525411	0.0
11	0.0	0.0	0.0	1.2040498128773491	0.0
12	0.0	0.0	0.0	1.3918435551737791	0.0
13	0.0	0.0	0.0	1.465551760160883	0.0
14	0.0	0.0	0.0	1.4948560844501413	0.0
15	0.0	0.0	0.0	1.5414969364677775	0.0
16	0.0	0.0	0.0	1.6442857686121606	0.0
17	0.0	0.0	0.0	1.765082219880859	0.0
18	0.0	0.0	0.0	1.9715546726975413	0.0
19	0.0	0.0	0.0	2.037880872329336	0.0
20	0.0	0.0	0.0	2.114944534296126	0.0
21	0.0	0.0	0.0	2.211917241009053	0.0
22	0.0	0.0	0.0	2.2999420624428173	0.0
23	0.0	0.0	0.0	2.415593479675997	0.0
24	0.0	0.0	0.0	2.4967955385844	0.0
25	0.0	0.0	0.0	2.553614610107084	0.0
26	0.0	0.0	0.0	2.6170327470231505	0.0
27	0.0	0.0	0.0	2.6783257611854157	0.0
28	0.0	0.0	0.0	2.7379410468578382	0.0
29	0.0	0.0	0.0	2.808070097733277	0.0
30	0.0	0.0	0.0	2.913766992593388	0.0
31	0.0	0.0	0.0	3.008390879420535	0.0
32	0.0	0.0	0.0	3.075723716146235	0.0
33	0.0	0.0	0.0	3.1430565528719354	0.0
34	0.0	0.0	0.0	3.2266074316661184	0.0
35	0.0	0.0	0.0	3.354785888290126	0.0
36	0.0	0.0	0.0	3.4449358324776918	0.0
37	0.0	0.0	0.0	3.5307236825916655	0.0
38	0.0	0.0	0.0	3.6119257415000683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAT	20	7.8555424E-4	44.0	7
TCGCCTG	20	7.8555424E-4	44.0	17
TCTGCGA	20	7.8555424E-4	44.0	1
AACCGTA	25	4.442045E-5	44.0	16
CGGGTAT	75	0.0	44.0	6
AGCGTAA	40	8.309144E-9	44.0	1
CTCACGT	20	7.8555424E-4	44.0	44
CTCACGA	150	0.0	44.0	24
CTCGTTA	25	4.442045E-5	44.0	1
ACGCCCG	25	4.442045E-5	44.0	10
GGTGTCG	25	4.442045E-5	44.0	8
CGAGTGA	20	7.8555424E-4	44.0	41
GGTCGCT	25	4.442045E-5	44.0	8
CCTAGTC	25	4.442045E-5	44.0	26
CGAAACA	20	7.8555424E-4	44.0	38
ACCGCTC	30	2.5270838E-6	44.0	18
GTTTACG	25	4.442045E-5	44.0	1
CGTTGTA	30	2.5270838E-6	44.0	1
TAGCGTG	20	7.8555424E-4	44.0	10
ATTACTA	40	8.309144E-9	44.0	1
>>END_MODULE