FastQCFastQC Report
Thu 26 May 2016
SRR1545729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545729_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences347182
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10640.30646750119533844No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.20997632365733243No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.1780046200551872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC551.8189894E-1245.00000425
TTAGGCA351.2086457E-745.0000045
TTAGGAC453.8380676E-1045.0000045
TTTACGG351.2086457E-745.0000042
TAGACGG351.2086457E-745.0000042
TAACGGG351.2086457E-745.0000043
GACGGTC551.8189894E-1245.00000429
ACTAACC551.8189894E-1245.00000414
CGGTCTA551.8189894E-1245.00000431
TGAATTC453.8380676E-1045.00000415
GGTCTAA551.8189894E-1245.00000432
AGTACGG351.2086457E-745.0000042
ACGACGG551.8189894E-1245.00000427
ATTTACG253.885162E-545.01
CTTCGCA207.026477E-445.028
TAGAGAT253.885162E-545.04
TTGGCCG253.885162E-545.036
ACTGCAA253.885162E-545.01
GGTGTTA253.885162E-545.01
GCGACCG207.026477E-445.032