##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545729_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	347182
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.056762735395267	31.0	31.0	33.0	30.0	33.0
2	31.209838067641755	31.0	31.0	33.0	30.0	34.0
3	31.15557834219516	31.0	31.0	34.0	28.0	34.0
4	35.07604944956824	35.0	35.0	37.0	32.0	37.0
5	32.78537769815255	35.0	35.0	37.0	28.0	37.0
6	33.98604766376137	35.0	35.0	37.0	28.0	37.0
7	15.09755690099141	0.0	0.0	35.0	0.0	37.0
8	24.834858950060774	17.0	17.0	35.0	17.0	37.0
9	32.80484299301231	32.0	32.0	37.0	27.0	39.0
10	35.663366764405986	37.0	35.0	37.0	32.0	39.0
11	36.5857763363308	37.0	35.0	39.0	33.0	39.0
12	36.115510020680794	37.0	35.0	39.0	32.0	39.0
13	35.42056039771647	37.0	35.0	39.0	30.0	39.0
14	36.413924108968786	38.0	35.0	40.0	30.0	41.0
15	36.97549124090535	39.0	35.0	41.0	32.0	41.0
16	37.17759273234211	39.0	35.0	41.0	32.0	41.0
17	37.18621645131372	39.0	35.0	41.0	32.0	41.0
18	37.09418114994441	38.0	35.0	40.0	32.0	41.0
19	36.98776722295511	38.0	35.0	40.0	32.0	41.0
20	37.06954853650247	39.0	35.0	40.0	32.0	41.0
21	36.83780553139275	38.0	35.0	40.0	32.0	41.0
22	36.9046494345905	38.0	35.0	40.0	32.0	41.0
23	36.918656497168634	38.0	35.0	40.0	32.0	41.0
24	36.75676446359546	38.0	35.0	40.0	32.0	41.0
25	36.50669965608816	38.0	35.0	40.0	31.0	41.0
26	36.37386730878905	38.0	35.0	40.0	31.0	41.0
27	36.24494069392998	38.0	34.0	40.0	31.0	41.0
28	36.25220201508143	38.0	35.0	40.0	31.0	41.0
29	36.356614110178526	38.0	35.0	40.0	31.0	41.0
30	36.095926632141065	38.0	35.0	40.0	30.0	41.0
31	35.91418621933165	38.0	34.0	40.0	30.0	41.0
32	35.739234752953784	38.0	34.0	40.0	30.0	41.0
33	35.52231682518103	38.0	34.0	40.0	29.0	41.0
34	35.4040416841887	38.0	34.0	40.0	29.0	41.0
35	35.24877153769493	38.0	34.0	40.0	27.0	41.0
36	35.00213144690681	37.0	34.0	40.0	26.0	41.0
37	34.793215086035566	37.0	34.0	40.0	25.0	41.0
38	34.808417487081705	37.0	34.0	40.0	25.0	41.0
39	34.74965291979423	37.0	34.0	40.0	25.0	41.0
40	34.56216336100374	37.0	33.0	40.0	24.0	41.0
41	34.48977193518097	37.0	33.0	40.0	23.0	41.0
42	34.46215817640316	37.0	33.0	40.0	23.0	41.0
43	34.37244442396207	37.0	33.0	40.0	23.0	41.0
44	34.13876007396697	36.0	33.0	40.0	23.0	41.0
45	34.00913929869636	36.0	33.0	40.0	23.0	41.0
46	34.02761087844416	36.0	33.0	40.0	23.0	41.0
47	34.04139327499698	36.0	33.0	40.0	23.0	41.0
48	34.0764210126101	36.0	33.0	40.0	23.0	41.0
49	34.010251107488294	36.0	33.0	40.0	23.0	41.0
50	33.840619617376476	36.0	33.0	40.0	23.0	41.0
51	33.6446964416358	35.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	20.0
16	45.0
17	131.0
18	208.0
19	461.0
20	808.0
21	1250.0
22	1870.0
23	2798.0
24	3950.0
25	5280.0
26	6236.0
27	6828.0
28	7207.0
29	8214.0
30	10251.0
31	13628.0
32	18695.0
33	29837.0
34	37625.0
35	35175.0
36	35721.0
37	56154.0
38	55917.0
39	8865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.443634750649515	24.807449694972668	30.59663231388724	15.152283240490577
2	31.367409600728145	24.07354067895225	32.43658945452241	12.12246026579719
3	29.74491765126072	22.962884020484932	33.852849514087715	13.439348814166632
4	26.34209146787564	28.048401126786526	31.00045509271794	14.609052312619895
5	21.10823717819472	37.29023970136701	29.19102948885599	12.410493631582282
6	19.68103185072959	40.5478970683964	29.199670489829543	10.571400591044466
7	37.804091225927614	57.46092827393126	4.056085856985673	0.6788946431554631
8	89.62964669827352	2.66171633322004	6.486223364114499	1.222413604391933
9	86.40223283465157	3.7095817179462065	8.003583135070366	1.8846023123318605
10	62.86184191576753	17.841938810191774	11.522486764866843	7.773732509173863
11	59.717669694857456	15.190015611408425	17.053879521403758	8.038435172330363
12	52.90193616028481	20.532458479990325	17.978754658939692	8.58685070078518
13	19.817271632745936	51.9399047185626	18.33389979895271	9.908923849738754
14	11.39056748333727	51.982821690064576	25.168355502301388	11.458255324296767
15	9.280435045595683	20.974877729836226	58.6695162767655	11.075170947802594
16	10.999130139235328	13.913451734248897	56.238226636173536	18.84919149034224
17	11.831546566354247	14.741835694246822	29.28723263302821	44.139385106370725
18	20.05835555990806	21.133296081018024	37.89395763605256	20.914390723021356
19	30.957826154581745	22.547251873657046	25.257933879060552	21.23698809270066
20	35.03609058073287	20.598994187486678	24.400746582484114	19.964168649296337
21	20.286766018975637	29.954893974918058	27.770448928803916	21.987891077302393
22	22.21946990339361	24.37280734600296	23.49142524670058	29.91629750390285
23	17.11839899533962	32.811896930140385	23.64293079710354	26.426773277416455
24	17.383965758593476	21.43573111509237	46.75847250145457	14.421830624859583
25	13.903946633177988	24.45720112217799	41.45577823735101	20.183074007293005
26	13.635211502900496	38.847636110167	27.76123186109879	19.755920525833712
27	14.942306916833244	39.00461429451988	28.604305522751755	17.44877326589512
28	11.482738160388498	29.431825382652328	43.88908411150348	15.196352345455697
29	11.806199630165159	23.31946932732688	42.197464154247626	22.676866888260335
30	16.479829023394068	33.27879901607802	32.807288396287824	17.434083564240083
31	32.66010334637164	26.590664262548174	23.27223185533812	17.47700053574206
32	34.45627941540748	24.89501183817133	25.648507123065134	15.00020162335605
33	30.212971870661498	27.77592156275383	23.552200286881234	18.45890627970344
34	17.832433709120863	26.501373919154798	28.781158009343805	26.885034362380537
35	19.19339136245543	24.60352207199682	33.08927306139143	23.113813504156322
36	34.807104054933724	23.66395723280585	24.827612030577622	16.701326681682808
37	19.458094025611928	35.26910957365301	28.623603758259357	16.649192642475704
38	19.30226797472219	35.538132737296294	23.601453992430482	21.558145295551036
39	19.91030640989452	34.26502526052618	26.2461763570692	19.578491972510097
40	25.259374045889476	25.04162082135595	23.586764290775445	26.112240841979133
41	14.875771209336888	23.033164161736497	28.03227125830256	34.05879337062405
42	21.39886284427188	24.32470577391685	22.728136827370083	31.548294554441185
43	22.294934645229304	25.93077982153453	24.33248267479305	27.441802858443122
44	18.978230438213963	32.192337160336656	27.764400228122426	21.06503217332696
45	15.517509548306077	42.59955873288362	19.768593993928256	22.11433772488205
46	21.003681066414735	36.19081634416531	24.973932980396448	17.83156960902351
47	20.97747003012829	27.497969364771212	26.65547176984982	24.869088835250675
48	22.602554279887784	22.2505775068984	33.09445766197556	22.052410551238253
49	20.961340161644326	22.82693227183437	33.940411657286376	22.271315909234925
50	17.849427677702185	36.79223001192458	25.09001042680784	20.26833188356539
51	16.356838776203837	37.7637665547177	22.654112252363316	23.22528241671515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	319.0
1	346.0
2	373.0
3	1454.0
4	2535.0
5	2042.0
6	1549.0
7	1681.0
8	1813.0
9	1921.5
10	2030.0
11	2025.0
12	2020.0
13	1983.0
14	1946.0
15	1834.0
16	1722.0
17	1609.0
18	1496.0
19	1404.5
20	1313.0
21	1175.0
22	1037.0
23	1120.5
24	1204.0
25	1400.0
26	1787.5
27	1979.0
28	2675.5
29	3372.0
30	4234.5
31	5097.0
32	5903.5
33	6710.0
34	7551.5
35	8393.0
36	9999.5
37	11606.0
38	17191.5
39	22777.0
40	30111.5
41	37446.0
42	39582.5
43	41719.0
44	39023.0
45	36327.0
46	34540.0
47	32753.0
48	30167.0
49	27581.0
50	25091.0
51	22601.0
52	20142.5
53	17684.0
54	15371.5
55	13059.0
56	11668.5
57	10278.0
58	9226.0
59	8174.0
60	7215.5
61	6257.0
62	5371.5
63	4486.0
64	3609.0
65	2732.0
66	2184.0
67	1636.0
68	1447.5
69	1259.0
70	1090.5
71	922.0
72	774.0
73	626.0
74	484.5
75	260.5
76	178.0
77	140.5
78	103.0
79	80.5
80	58.0
81	44.0
82	30.0
83	28.0
84	26.0
85	16.0
86	6.0
87	4.5
88	3.0
89	4.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.840337344678005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62172706296747	18.507295885155337
2	6.088303813271209	3.1464764878363507
3	3.2303010711936953	2.5041620821355948
4	2.2505099595376366	2.3261574620804075
5	1.6831451406150726	2.174651911677449
6	1.3476307781480945	2.0893940354050615
7	1.1481056257175661	2.076720567310517
8	0.9942817651845329	2.0554060982424205
9	0.8371139076833903	1.9468175193414408
>10	10.483430495023018	57.00727572281973
>50	0.29092773622551915	4.759175302867084
>100	0.021178647464692966	0.7120184802207488
>500	0.0022293313120729436	0.3879809437125196
>1k	0.0011146656560364718	0.30646750119533844
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1064	0.30646750119533844	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.20997632365733243	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.1780046200551872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008641000973552776	0.0
2	0.0	0.0	0.0	0.03341187043107074	0.0
3	0.0	0.0	0.0	0.04147680467305333	0.0
4	0.0	0.0	0.0	0.06307930710693527	0.0
5	0.0	0.0	0.0	0.09937151119585692	0.0
6	0.0	0.0	0.0	0.1509294836713885	0.0
7	0.0	0.0	0.0	0.18693365439452506	0.0
8	0.0	0.0	0.0	0.4798635873979642	0.0
9	0.0	0.0	0.0	0.600837601027703	0.0
10	0.0	0.0	0.0	0.8024609570772678	0.0
11	0.0	0.0	0.0	0.9712485094273321	0.0
12	0.0	0.0	0.0	1.108064358175251	0.0
13	0.0	0.0	0.0	1.1688393983559056	0.0
14	0.0	0.0	0.0	1.1910179675213577	0.0
15	0.0	0.0	0.0	1.2255819714155687	0.0
16	0.0	0.0	0.0	1.3076714806643202	0.0
17	0.0	0.0	0.0	1.4064669251286068	0.0
18	0.0	0.0	0.0	1.590808279231066	0.0
19	0.0	0.0	0.0	1.6512952860459356	0.0
20	0.0	0.0	0.0	1.7207113272001429	0.0
21	0.0	0.0	0.0	1.8076974036672409	0.0
22	0.0	0.0	0.0	1.870200644042606	0.0
23	0.0	0.0	0.0	1.973028555627884	0.0
24	0.0	0.0	0.0	2.0418685300505213	0.0
25	0.0	0.0	0.0	2.0844974681867146	0.0
26	0.0	0.0	0.0	2.1366315073938167	0.0
27	0.0	0.0	0.0	2.1884775132351333	0.0
28	0.0	0.0	0.0	2.244067952831656	0.0
29	0.0	0.0	0.0	2.3031147928176	0.0
30	0.0	0.0	0.0	2.387508568992632	0.0
31	0.0	0.0	0.0	2.4592288770731203	0.0
32	0.0	0.0	0.0	2.5200039172537747	0.0
33	0.0	0.0	0.0	2.5790507572397185	0.0
34	0.0	0.0	0.0	2.6588359995621893	0.0
35	0.0	0.0	0.0	2.7720331123157305	0.0
36	0.0	0.0	0.0	2.844905553859359	0.0
37	0.0	0.0	0.0	2.9125933948188556	0.0
38	0.0	0.0	0.0	2.991514537043971	0.0
39	0.0	0.0	0.0	3.0666912455138804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	55	1.8189894E-12	45.000004	25
TTAGGCA	35	1.2086457E-7	45.000004	5
TTAGGAC	45	3.8380676E-10	45.000004	5
TTTACGG	35	1.2086457E-7	45.000004	2
TAGACGG	35	1.2086457E-7	45.000004	2
TAACGGG	35	1.2086457E-7	45.000004	3
GACGGTC	55	1.8189894E-12	45.000004	29
ACTAACC	55	1.8189894E-12	45.000004	14
CGGTCTA	55	1.8189894E-12	45.000004	31
TGAATTC	45	3.8380676E-10	45.000004	15
GGTCTAA	55	1.8189894E-12	45.000004	32
AGTACGG	35	1.2086457E-7	45.000004	2
ACGACGG	55	1.8189894E-12	45.000004	27
ATTTACG	25	3.885162E-5	45.0	1
CTTCGCA	20	7.026477E-4	45.0	28
TAGAGAT	25	3.885162E-5	45.0	4
TTGGCCG	25	3.885162E-5	45.0	36
ACTGCAA	25	3.885162E-5	45.0	1
GGTGTTA	25	3.885162E-5	45.0	1
GCGACCG	20	7.026477E-4	45.0	32
>>END_MODULE