FastQCFastQC Report
Thu 26 May 2016
SRR1545728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545728_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1157468
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.4434679835641244No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.19940076097136164No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.1843679479691879No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17920.15482069482698443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA302.5277313E-644.0000041
ACTTACG207.8565034E-444.00000431
CGTACAT207.8565034E-444.00000435
TTACGTT207.8565034E-444.00000433
CGGTATC207.8565034E-444.00000433
AATCGCG302.5277313E-644.00000419
CCCGTAT351.4460966E-744.034
TATTACG254.44286E-544.01
GTCCGAT254.44286E-544.042
CAATCGA351.4460966E-744.029
CGTTTTA27050.043.024031
CGTTATT15250.041.9803281
AGGCGAT8700.040.4597667
CTACGGG1950.039.487183
GTTTTAT32550.039.403992
ATATACG452.3510438E-839.111111
ATAGGCG1300.038.923085
ATAACGC850.038.8235311
CCGTATG404.1245767E-738.50000435
TCAATCG404.1245767E-738.50000428
Produced by FastQC (version 0.11.5)