##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545728_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1157468
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522643390573215	31.0	31.0	33.0	30.0	34.0
2	31.7133691816966	31.0	31.0	34.0	30.0	34.0
3	31.76613867510808	31.0	31.0	34.0	30.0	34.0
4	35.67728870258184	37.0	35.0	37.0	35.0	37.0
5	27.018523190273942	35.0	22.0	37.0	0.0	37.0
6	31.294496262531663	35.0	25.0	37.0	17.0	37.0
7	15.105904439690773	0.0	0.0	32.0	0.0	37.0
8	25.14972508959211	17.0	17.0	35.0	17.0	37.0
9	33.185317434261684	32.0	32.0	37.0	28.0	39.0
10	36.018039375602605	37.0	35.0	39.0	32.0	39.0
11	36.99893560772306	39.0	37.0	39.0	34.0	39.0
12	37.068348325828445	39.0	35.0	39.0	34.0	39.0
13	36.64738290820999	39.0	35.0	39.0	33.0	39.0
14	37.94673891632425	40.0	37.0	41.0	33.0	41.0
15	38.19268610449706	40.0	37.0	41.0	33.0	41.0
16	38.25539885335923	40.0	37.0	41.0	34.0	41.0
17	38.273145348294726	40.0	37.0	41.0	34.0	41.0
18	38.242754011342	40.0	37.0	41.0	34.0	41.0
19	38.19640197396386	40.0	37.0	41.0	34.0	41.0
20	38.231139003410895	40.0	37.0	41.0	34.0	41.0
21	38.09525706110234	40.0	36.0	41.0	34.0	41.0
22	38.011410250650556	40.0	36.0	41.0	34.0	41.0
23	37.92384325095813	40.0	36.0	41.0	34.0	41.0
24	37.78089675049332	40.0	35.0	41.0	33.0	41.0
25	37.60564006953108	39.0	35.0	41.0	33.0	41.0
26	37.35843496321281	39.0	35.0	40.0	33.0	41.0
27	37.163410997107476	39.0	35.0	40.0	33.0	41.0
28	37.200586106916134	39.0	35.0	40.0	33.0	41.0
29	37.13148700439235	39.0	35.0	40.0	33.0	41.0
30	36.925571160498606	39.0	35.0	40.0	32.0	41.0
31	36.74096735287714	38.0	35.0	40.0	32.0	41.0
32	36.54109228073692	38.0	35.0	40.0	31.0	41.0
33	36.3294829749073	38.0	35.0	40.0	31.0	41.0
34	36.07576278566665	38.0	35.0	40.0	30.0	41.0
35	35.88378598803595	38.0	35.0	40.0	30.0	41.0
36	35.701559783942194	38.0	35.0	40.0	29.0	41.0
37	35.591600804514684	38.0	35.0	40.0	29.0	41.0
38	35.40233768881731	38.0	34.0	40.0	28.0	41.0
39	35.33438419031887	38.0	34.0	40.0	27.0	41.0
40	35.09048111913245	38.0	34.0	40.0	27.0	41.0
41	34.88397346622109	38.0	34.0	40.0	26.0	41.0
42	34.88792519534017	38.0	34.0	40.0	26.0	41.0
43	34.78349811830651	37.0	34.0	40.0	26.0	41.0
44	34.57087366562186	37.0	34.0	40.0	25.0	40.0
45	34.38626726613608	37.0	33.0	40.0	25.0	40.0
46	34.31913798048844	37.0	33.0	39.0	24.0	40.0
47	34.13576530841458	36.0	33.0	39.0	24.0	40.0
48	34.03342727401535	36.0	33.0	39.0	24.0	40.0
49	33.909723638148094	36.0	33.0	39.0	24.0	40.0
50	33.990578573230536	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	10.0
14	9.0
15	24.0
16	48.0
17	163.0
18	349.0
19	753.0
20	1424.0
21	2507.0
22	4153.0
23	6060.0
24	9435.0
25	13709.0
26	17365.0
27	19041.0
28	20138.0
29	22641.0
30	27541.0
31	36915.0
32	51107.0
33	88015.0
34	94421.0
35	120759.0
36	164337.0
37	294950.0
38	127813.0
39	33777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.15604751060072	22.928409251918843	26.98078910172895	14.934754135751485
2	34.907833305110806	23.350969530043166	28.937387469891174	12.803809694954849
3	31.179004516755537	23.29723154333424	32.342233219406495	13.18153072050372
4	26.893270483503645	26.446001098950468	31.135893173720568	15.524835243825317
5	17.37490798881697	47.84451924372855	23.25757601938023	11.522996748074245
6	20.89975705591861	40.34910684355852	28.901360555972172	9.849775544550692
7	39.48541125974973	55.34641130467538	4.137133812770634	1.031043622804259
8	86.7450331240259	3.185228446920347	8.231329073460346	1.838409355593416
9	81.96563533505893	4.808340273769987	10.167797295475987	3.058227095695086
10	46.60413938009517	24.119198111740452	16.094354228367436	13.182308279796937
11	39.553490895644636	22.76926878324066	22.159230319974288	15.51801000114042
12	34.760097039399795	21.62634301769034	27.602663745347606	16.010896197562268
13	23.144916317340954	31.807618007582068	28.582647641230686	16.464818033846292
14	18.518092940798365	34.21761983916618	31.289072354484098	15.97521486555136
15	17.12418831449336	23.16089948059039	43.02304685745092	16.691865347465328
16	20.086343639737773	19.122947675443296	40.99033407403056	19.800374610788378
17	20.599532773260254	19.032405215522157	30.001520560395623	30.366541450821966
18	23.523069320274946	22.36580190553864	33.07659477411038	21.03453400007603
19	28.426099036863224	23.81819626978888	27.27652081958205	20.47918387376584
20	30.012319995023624	23.754090825837086	25.955101998500176	20.278487180639118
21	25.479149315575032	25.277243085769975	29.631920709687005	19.611686888967988
22	24.02243517747359	22.261695355724736	28.376335242097404	25.33953422470427
23	22.08769486499843	27.411988927555665	27.409569854198995	23.090746353246917
24	21.832396230392547	24.637570973884376	33.89216807721682	19.637864718506258
25	22.11179920308812	23.680136297504554	32.57256356115244	21.635500938254882
26	20.238399679299988	28.786540967007294	28.30013443136225	22.674924922330465
27	20.16729620170925	27.976237787999324	30.671431089239615	21.185034921051813
28	18.95076148973449	25.891774113841592	35.468626346473506	19.68883804995041
29	19.93869376950378	25.017883863743968	34.52389180521621	20.519530561536044
30	22.678639927842497	26.047631554392865	31.344279064302427	19.92944945346221
31	28.998901049532254	23.902863837272392	26.710803236028983	20.387431877166367
32	28.7519827761977	25.29694125453144	27.34788348360387	18.60319248566699
33	27.775886676780697	24.561024581241124	27.023122885470702	20.63996585650748
34	21.87153338148441	26.85344216859559	28.963824485860517	22.31119996405948
35	22.574792564459663	25.43750669564947	30.16515359387905	21.82254714601181
36	28.96373809038349	25.090628855398162	26.023527216303172	19.922105837915176
37	22.659373736466147	29.768339167907882	26.79979057736369	20.772496518262276
38	23.154419819813594	30.400667664246438	24.46322490124997	21.981687614689996
39	22.66481665151866	28.123455680848195	25.752677395832972	23.459050271800173
40	24.35877276952797	25.20916344987507	27.060532127022086	23.371531653574873
41	20.50363379376363	24.570441688236738	27.58693976852924	27.338984749470395
42	22.911994111284287	26.13653250025055	25.773325914841706	25.178147473623465
43	23.201505354791667	25.733324808979603	26.51753655392633	24.547633282302407
44	21.665134586874107	27.268140458310725	29.049442403591286	22.01728255122388
45	20.836601962214075	30.74780469092882	25.857475109463067	22.558118237394037
46	23.58043591701887	28.465668165340208	27.509961398500867	20.443934519140054
47	22.582308970960753	27.106408125321824	27.58287918110911	22.728403722608313
48	22.975926764282036	26.162278352403696	29.132036479626215	21.729758403688052
49	22.043373985285122	25.454613000100217	30.237380212671106	22.264632801943552
50	20.83478765719657	29.504746567507702	27.6966620243497	21.96380375094603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	646.0
1	1291.5
2	1937.0
3	8936.0
4	15935.0
5	11237.0
6	6539.0
7	6576.5
8	6614.0
9	6671.5
10	6729.0
11	6623.0
12	6517.0
13	6341.5
14	6166.0
15	5777.0
16	5388.0
17	5286.0
18	5184.0
19	5295.5
20	5407.0
21	5559.0
22	5711.0
23	6156.0
24	6601.0
25	7081.0
26	7561.0
27	9064.0
28	10567.0
29	12827.0
30	15087.0
31	17919.0
32	20751.0
33	23633.0
34	26515.0
35	29178.5
36	31842.0
37	35373.5
38	38905.0
39	49179.5
40	59454.0
41	71216.0
42	82978.0
43	87279.0
44	91580.0
45	92693.0
46	93806.0
47	92609.0
48	91412.0
49	90057.0
50	88702.0
51	82188.5
52	75675.0
53	70578.0
54	65481.0
55	62504.5
56	59528.0
57	56931.0
58	54334.0
59	51261.5
60	48189.0
61	42558.0
62	36927.0
63	31702.0
64	26477.0
65	22512.5
66	18548.0
67	15647.5
68	12747.0
69	10896.5
70	9046.0
71	7299.5
72	5553.0
73	4230.5
74	2908.0
75	2327.0
76	1746.0
77	1342.0
78	938.0
79	702.0
80	466.0
81	343.0
82	220.0
83	145.0
84	70.0
85	50.0
86	30.0
87	30.5
88	31.0
89	21.0
90	11.0
91	8.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1157468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.762958849575888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.95745388935957	17.765480469230976
2	7.119676300235558	3.6684785509053888
3	3.7353099130807985	2.8869790674334053
4	2.5623946207905215	2.640594686872033
5	1.9733343873976252	2.5419466309489036
6	1.6068521849253887	2.4838360030550346
7	1.3897240912726883	2.5062383203005765
8	1.1933850942285398	2.45961048594457
9	1.0043719095840897	2.328803295865638
>10	10.062405526171448	52.01373391085314
>50	0.3521392900195875	5.611853609519752
>100	0.04110137944573084	1.9599062803335183
>500	3.702826977088403E-4	0.04817486841410315
>1k	0.001110848093126521	0.5946972865218199
>5k	3.702826977088403E-4	0.4896665338011712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5133	0.4434679835641244	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2308	0.19940076097136164	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2134	0.1843679479691879	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1792	0.15482069482698443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008985129610494632	0.0
2	0.0	0.0	0.0	0.03032481243541938	0.0
3	0.0	0.0	0.0	0.03982831490805793	0.0
4	0.0	0.0	0.0	0.052182868122488055	0.0
5	0.0	0.0	0.0	0.0885553639495865	0.0
6	0.0	0.0	0.0	0.16259628775914323	0.0
7	0.0	0.0	0.0	0.21106415036959986	0.0
8	0.0	0.0	0.0	0.4561681186866505	0.0
9	0.0	0.0	0.0	0.5685686342948574	0.0
10	0.0	0.0	0.0	0.7596754294719162	0.0
11	0.0	0.0	0.0	0.9210621805527237	0.0
12	0.0	0.0	0.0	1.0650834407517098	0.0
13	0.0	0.0	0.0	1.1263378339617165	0.0
14	0.0	0.0	0.0	1.1525156634999845	0.0
15	0.0	0.0	0.0	1.1851731538150514	0.0
16	0.0	0.0	0.0	1.2640522243379515	0.0
17	0.0	0.0	0.0	1.3545082887820656	0.0
18	0.0	0.0	0.0	1.4965424530095	0.0
19	0.0	0.0	0.0	1.5588335919437946	0.0
20	0.0	0.0	0.0	1.6276043916548881	0.0
21	0.0	0.0	0.0	1.7181468515760263	0.0
22	0.0	0.0	0.0	1.8057518652783489	0.0
23	0.0	0.0	0.0	1.9052794548099818	0.0
24	0.0	0.0	0.0	1.9808754972059703	0.0
25	0.0	0.0	0.0	2.048436760238728	0.0
26	0.0	0.0	0.0	2.115134068501246	0.0
27	0.0	0.0	0.0	2.1873606872932987	0.0
28	0.0	0.0	0.0	2.2627839387352395	0.0
29	0.0	0.0	0.0	2.3480562745579143	0.0
30	0.0	0.0	0.0	2.4589016715796896	0.0
31	0.0	0.0	0.0	2.555146232984411	0.0
32	0.0	0.0	0.0	2.638690659266606	0.0
33	8.639547702398684E-5	0.0	0.0	2.729492305618816	0.0
34	8.639547702398684E-5	0.0	0.0	2.8327349006624805	0.0
35	8.639547702398684E-5	0.0	0.0	2.958872297117501	0.0
36	8.639547702398684E-5	0.0	0.0	3.064274779086765	0.0
37	8.639547702398684E-5	0.0	0.0	3.1614696907387505	0.0
38	8.639547702398684E-5	0.0	0.0	3.294777911786762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	30	2.5277313E-6	44.000004	1
ACTTACG	20	7.8565034E-4	44.000004	31
CGTACAT	20	7.8565034E-4	44.000004	35
TTACGTT	20	7.8565034E-4	44.000004	33
CGGTATC	20	7.8565034E-4	44.000004	33
AATCGCG	30	2.5277313E-6	44.000004	19
CCCGTAT	35	1.4460966E-7	44.0	34
TATTACG	25	4.44286E-5	44.0	1
GTCCGAT	25	4.44286E-5	44.0	42
CAATCGA	35	1.4460966E-7	44.0	29
CGTTTTA	2705	0.0	43.02403	1
CGTTATT	1525	0.0	41.980328	1
AGGCGAT	870	0.0	40.459766	7
CTACGGG	195	0.0	39.48718	3
GTTTTAT	3255	0.0	39.40399	2
ATATACG	45	2.3510438E-8	39.11111	1
ATAGGCG	130	0.0	38.92308	5
ATAACGC	85	0.0	38.82353	11
CCGTATG	40	4.1245767E-7	38.500004	35
TCAATCG	40	4.1245767E-7	38.500004	28
>>END_MODULE